PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

MDP0000136498

Organism

Malus domestica

Taxonomic ID

3750

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Malus

Family

bHLH

Protein Properties

Length: 759aa MW: 83675.7 Da PI: 6.0259

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MDP0000136498	genome	GDR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	38.6	1.9e-12	580	625	4	54
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 +h e+Er+RR+++N++f Lr ++P+ K++Ka+ L A+ YI++L MDP0000136498 580 NHVEAERQRREKLNQRFYALRAVVPNV-----SKMDKASLLGDAIAYINEL 625 799*********************6.....5*************998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	3.1E-54	153	334	IPR025610	Transcription factor MYC/MYB N-terminal
PROSITE profile	PS50888	17.111	576	625	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	5.5E-18	579	646	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	6.66E-15	579	630	No hit	No description
Pfam	PF00010	6.6E-10	580	625	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	3.4E-18	580	646	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	2.3E-16	582	631	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 759 aa Download sequence Send to blast
MPSWKFLLGP LTLSSFTSHF PPQLNLQFKC TPHKIPLRNS IPSPTQXXPS LSLSLSLYTP 60 NQSHPQNAAY HMTDYRIPPT ANMWTDDNAS LMDAFINSSD LTSFWAAPPA QPTLQPAHAP 120 VQPQSSASTS DYPKPPVAAX FQPSVTPFNQ ETLMQRLQAL IDGARESWTY AIFWQSSYDY 180 SGAAVLGWGE GFYKDERDKV KAKAKTTTSA AEQEYRKKVL RDLNSLISGA DTSADDDAVD 240 QEVTDTEWFF LVSMTQSFVN GGGLPGQAFF HSTPVWVAGP DRLAASPCER ARQGQVFGLQ 300 TMVCVPTANG VVELGSTELI YQSSDLMNKV RVLFDFNNLE VGSWPMSGGA TADQGENDPS 360 SLWLNDPSTT TMEMKDPVNT SATTNTSNQL ISKPVQFDNH PSSSSLSENP SPIQVPQLQQ 420 QVQQQQQTQS FFTGELNFSD YNVYDGSSVK NSNSNSNSHS LKPESGEILN FGESKRSSYS 480 ANGKLFLGHS QMTAAEDNNS KKKRSPPSRG SNDEGILSFS SGVILPSSCV VKSSGGADSD 540 HSDLEASVVR EADSSRVVDQ EKRPRKRGRK PANGREEPLN HVEAERQRRE KLNQRFYALR 600 AVVPNVSKMD KASLLGDAIA YINELKLKLQ TVETDKEELQ NQLESMNKDL PCKDSRSSGS 660 IMSEDELKGC SSKLLDMDID VKIIGRDAMI RIQCCKKNHP AARLMAALKE LDMDVHYASV 720 SVVNDLMIQQ ATVKMGSRIY TQDHLRLALH SKVGDNRQT

Sequence ? help Back to Top

Protein Sequence Length: 759 aa Download sequence Send to blast

MPSWKFLLGP LTLSSFTSHF PPQLNLQFKC TPHKIPLRNS IPSPTQXXPS LSLSLSLYTP  60
NQSHPQNAAY HMTDYRIPPT ANMWTDDNAS LMDAFINSSD LTSFWAAPPA QPTLQPAHAP  120
VQPQSSASTS DYPKPPVAAX FQPSVTPFNQ ETLMQRLQAL IDGARESWTY AIFWQSSYDY  180
SGAAVLGWGE GFYKDERDKV KAKAKTTTSA AEQEYRKKVL RDLNSLISGA DTSADDDAVD  240
QEVTDTEWFF LVSMTQSFVN GGGLPGQAFF HSTPVWVAGP DRLAASPCER ARQGQVFGLQ  300
TMVCVPTANG VVELGSTELI YQSSDLMNKV RVLFDFNNLE VGSWPMSGGA TADQGENDPS  360
SLWLNDPSTT TMEMKDPVNT SATTNTSNQL ISKPVQFDNH PSSSSLSENP SPIQVPQLQQ  420
QVQQQQQTQS FFTGELNFSD YNVYDGSSVK NSNSNSNSHS LKPESGEILN FGESKRSSYS  480
ANGKLFLGHS QMTAAEDNNS KKKRSPPSRG SNDEGILSFS SGVILPSSCV VKSSGGADSD  540
HSDLEASVVR EADSSRVVDQ EKRPRKRGRK PANGREEPLN HVEAERQRRE KLNQRFYALR  600
AVVPNVSKMD KASLLGDAIA YINELKLKLQ TVETDKEELQ NQLESMNKDL PCKDSRSSGS  660
IMSEDELKGC SSKLLDMDID VKIIGRDAMI RIQCCKKNHP AARLMAALKE LDMDVHYASV  720
SVVNDLMIQQ ATVKMGSRIY TQDHLRLALH SKVGDNRQT

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4rqw_A	4e-73	148	337	4	195	Transcription factor MYC3
4rqw_B	4e-73	148	337	4	195	Transcription factor MYC3
4rs9_A	4e-73	148	337	4	195	Transcription factor MYC3
4yz6_A	4e-73	148	337	4	195	Transcription factor MYC3
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	561	569	KRPRKRGRK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Mdo.12247	0.0	bud\| fruit\| leaf\| root\| stem

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed at low levels in roots, stems, leaves, flowers and fruits. {ECO:0000269\|PubMed:15231736}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator that binds to the G-box motif (5'-AACGTG-3') found in the promoter of the jasmonate-induced gene LAPA1 (PubMed:15231736). Acts as negative regulator of blue light-mediated photomorphogenesis and positively regulates root growth (PubMed:24483714). Promotes growth in response to the phytohormones abscisic acid (ABA) and jasmonate (JA) (PubMed:24483714). Binds to the G-box motif (5'-CACGTG-3') of the RBCS-3A gene promoter (PubMed:24483714). Acts downstream of the jasmonate (JA) receptor to orchestrate JA-mediated activation of plant responses (PubMed:28733419). Positively regulates both wound-responsive and pathogen-responsive genes through MYC2-targeted transcription factors (MTFs) involved in early response to JA (PubMed:28733419). With JA2L forms a transcription module that regulates wounding-responsive genes (PubMed:28733419). With ERF.C3 forms a transcription module that regulates pathogen-responsive genes (PubMed:28733419). Plays a critical role in orchestrating JA-mediated defense gene expression during Botrytis cinerea infection (PubMed:28733419). Regulates negatively defense responses to root-knot nematodes, potentially by mediating crosstalk among the hormones strigolactones, abscisic acid (ABA) and jasmonate (JA) (PubMed:30576511). Regulates the termination of JA-mediated defense responses by specifically binding the G-box (5'-CACATG-3') motifs in the promoters of MTB1, MTB2 and MTB3, which are transcription factors that negatively regulates JA signaling (PubMed:30610166). May be involved in JA-induced chilling tolerance, possibly by ameliorating the antioxidant enzyme system of fruit and increasing proline and lycopene levels (PubMed:29528226). {ECO:0000269\|PubMed:15231736, ECO:0000269\|PubMed:24483714, ECO:0000269\|PubMed:28733419, ECO:0000269\|PubMed:29528226, ECO:0000269\|PubMed:30576511, ECO:0000269\|PubMed:30610166}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by methyl jasmonate (PubMed:15231736, PubMed:28733419). Induced by wounding (PubMed:15231736). Induced by infection with the fungal pathogen Botrytis cinerea (PubMed:28733419). Induced in fruit by storage in cold (PubMed:29528226). Induced by hydrogen peroxide and infection with root-knot nematodes (PubMed:30576511). {ECO:0000269\|PubMed:15231736, ECO:0000269\|PubMed:28733419, ECO:0000269\|PubMed:29528226, ECO:0000269\|PubMed:30576511}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_008341963.2	0.0	transcription factor MYC2-like
Swissprot	A0A3Q7HRZ6	0.0	MYC2_SOLLC; Transcription factor MYC2
TrEMBL	A0A498HHL4	0.0	A0A498HHL4_MALDO; Uncharacterized protein
STRING	XP_008341963.1	0.0	(Malus domestica)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF2938	33	64

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G32640.1	0.0	bHLH family protein

Link Out ? help Back to Top
Phytozome	MDP0000136498

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Gupta N,Prasad VB,Chattopadhyay S LeMYC2 acts as a negative regulator of blue light mediated photomorphogenic growth, and promotes the growth of adult tomato plants. BMC Plant Biol., 2014. 14: p. 38 [PMID:24483714] Sun H,Fan HJ,Ling HQ Genome-wide identification and characterization of the bHLH gene family in tomato. BMC Genomics, 2015. 16: p. 9 [PMID:25612924] Du M, et al. MYC2 Orchestrates a Hierarchical Transcriptional Cascade That Regulates Jasmonate-Mediated Plant Immunity in Tomato. Plant Cell, 2017. 29(8): p. 1883-1906 [PMID:28733419] Min D, et al. SlMYC2 Involved in Methyl Jasmonate-Induced Tomato Fruit Chilling Tolerance. J. Agric. Food Chem., 2018. 66(12): p. 3110-3117 [PMID:29528226] Xu X, et al. Strigolactones positively regulate defense against root-knot nematodes in tomato. J. Exp. Bot., 2019. 70(4): p. 1325-1337 [PMID:30576511] Liu Y, et al. MYC2 Regulates the Termination of Jasmonate Signaling via an Autoregulatory Negative Feedback Loop. Plant Cell, 2019. 31(1): p. 106-127 [PMID:30610166]