PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Loteae; Lotus

Family

WRKY

Protein Properties

Length: 490aa MW: 53033.1 Da PI: 4.7244

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Lj0g3v0272799.1	genome	Kazusa	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	101.5	4.9e-32	293	350	2	60
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 +DgynWrKYGqK+vkgs++prsYY+Ct+++C+vkkkvers+ +++++ei+Y+g Hnh+k Lj0g3v0272799.1 293 EDGYNWRKYGQKQVKGSDYPRSYYKCTHPNCQVKKKVERSH-EGHITEIIYKGAHNHPK 350 7**************************************.*************85 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.0E-28	289	351	IPR003657	WRKY domain
SuperFamily	SSF118290	9.29E-26	291	351	IPR003657	WRKY domain
SMART	SM00774	6.1E-35	292	350	IPR003657	WRKY domain
Pfam	PF03106	9.8E-25	293	349	IPR003657	WRKY domain
PROSITE profile	PS50811	23.043	293	351	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009555	Biological Process	pollen development
GO:0009942	Biological Process	longitudinal axis specification
GO:0030010	Biological Process	establishment of cell polarity
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 490 aa Download sequence Send to blast
MAGIDDNVAL TGDWGLHSPS PRTLFSRMLE EDNVTRSISE HPGSGRTGDL FPGTHDSSET 60 GRANMRESSQ DGNSGVQLTD PSFQTEQKSN SNSRGGLVER MAARAGFNAP RLNTESIRST 120 DLSLNSDIRS PYLTIPPGLS PTTLLDSPVF LANSLAQPSP TTGKFLFTSN GNIRCSELSS 180 DAPEKRKDNG FDDFYASSFA FKPTTDSGSS FYHDAGRKTN QTTLPQQSLP GIEASVQSRP 240 SFQSQSVDAV KVQTENKSGL EHSAPLEEQA DEEGDQRVNV DTVAAGVGGA PSEDGYNWRK 300 YGQKQVKGSD YPRSYYKCTH PNCQVKKKVE RSHEGHITEI IYKGAHNHPK PPPNRRSSGL 360 GSVNPHTDMQ VDNPEHVEPQ RVGEGDLGWA DAQKGNIAGA GNWKQDNLEV TSSASVGPEY 420 GNQSTNLQTQ NGTNLDSGEA VDGSSTFSNE EEEDDQGTHG SVSLGYDGEG DESESKRRYL 480 YYSASSFFES

Sequence ? help Back to Top

Protein Sequence Length: 490 aa Download sequence Send to blast

MAGIDDNVAL TGDWGLHSPS PRTLFSRMLE EDNVTRSISE HPGSGRTGDL FPGTHDSSET  60
GRANMRESSQ DGNSGVQLTD PSFQTEQKSN SNSRGGLVER MAARAGFNAP RLNTESIRST  120
DLSLNSDIRS PYLTIPPGLS PTTLLDSPVF LANSLAQPSP TTGKFLFTSN GNIRCSELSS  180
DAPEKRKDNG FDDFYASSFA FKPTTDSGSS FYHDAGRKTN QTTLPQQSLP GIEASVQSRP  240
SFQSQSVDAV KVQTENKSGL EHSAPLEEQA DEEGDQRVNV DTVAAGVGGA PSEDGYNWRK  300
YGQKQVKGSD YPRSYYKCTH PNCQVKKKVE RSHEGHITEI IYKGAHNHPK PPPNRRSSGL  360
GSVNPHTDMQ VDNPEHVEPQ RVGEGDLGWA DAQKGNIAGA GNWKQDNLEV TSSASVGPEY  420
GNQSTNLQTQ NGTNLDSGEA VDGSSTFSNE EEEDDQGTHG SVSLGYDGEG DESESKRRYL  480
YYSASSFFES

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	2e-17	294	352	16	75	WRKY transcription factor 1
Search in ModeBase

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Low expression in senescent leaves (PubMed:11722756). Expressed in both the unfertilized egg cell and the pollen tube (PubMed:21316593). {ECO:0000269\|PubMed:11722756, ECO:0000269\|PubMed:21316593}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Regulates WOX8 and WOX9 expression and basal cell division patterns during early embryogenesis. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Required to repolarize the zygote from a transient symmetric state. {ECO:0000269\|PubMed:21316593}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00071	PBM	Transfer from AT5G56270	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Lj0g3v0272799.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_027333911.1	0.0	probable WRKY transcription factor 2
Swissprot	Q9FG77	1e-106	WRKY2_ARATH; Probable WRKY transcription factor 2
TrEMBL	A0A445KGX3	0.0	A0A445KGX3_GLYSO; Putative WRKY transcription factor 2
TrEMBL	A0A4D6M2I7	0.0	A0A4D6M2I7_VIGUN; WRKY transcription factor 33
STRING	GLYMA06G47880.3	0.0	(Glycine max)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF9524	34	43

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G56270.1	1e-108	WRKY DNA-binding protein 2

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ueda M, et al. Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote. Genes Dev., 2017. 31(6): p. 617-627 [PMID:28404632] Lei R, et al. Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function. Plant J., 2017. 91(6): p. 962-976 [PMID:28635025]