PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; Saxifragales; Crassulaceae; Kalanchoe

Family

bHLH

Protein Properties

Length: 629aa MW: 68055.3 Da PI: 5.1127

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Kalax.0967s0002.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	36.8	7.1e-12	456	501	4	54
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 +h e+Er RR+++N++f Lr ++P+ K++Ka+ L A+ YI+ L Kalax.0967s0002.1.p 456 NHVEAERLRREKLNQKFYALRAVVPTV-----SKMDKASLLGDAISYINGL 501 799*********************6.....5*************988 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	1.2E-51	47	224	IPR025610	Transcription factor MYC/MYB N-terminal
PROSITE profile	PS50888	16.412	452	501	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.27E-17	455	529	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	8.4E-18	456	523	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	2.1E-9	456	501	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	5.68E-13	456	506	No hit	No description
SMART	SM00353	8.2E-15	458	507	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 629 aa Download sequence Send to blast
MVNLWIDDAF QYDLSAFWPL PSSSSTTAHL DPPNPQLQNN PFTQESLHQR LLALLDTTPT 60 QKWTYAIFWQ LSHSSTLGWG DGYYKGDDTK GQRRASDSTW LAEQDNRKKI LRELNSIISG 120 PGSVSLDDAV DEEVTDTEWF FLISMTQSFV NGDGLPGQAC LCSSPVWVVG EDLSSSSCER 180 ARQSRVFGIQ TMVCIPAVNG VVELGSTDLI FQSSDLMSRV RSLFHFEGFE SGNPWPVLSL 240 TGQDESGYDD QLAMLLTFSE PAQSVEMRDS GNGVGSSNHL AAAAAAAAAS SNVHVTNGNP 300 SAASCVTENA GYKVDIPIGT PNLLQGQNFG SAGNPDLHWV RPKSGENLSF GEILKTSSSC 360 GGLVPDDDFA QFVASGGDGD KRKKSAASRQ RKEEGILSFT PPAGGRVLKP AVAVKCGEHY 420 EGSVKEADCS RVVEPEKKQP KKRGRKPANG RDEPINHVEA ERLRREKLNQ KFYALRAVVP 480 TVSKMDKASL LGDAISYING LTAKLESLEA DVQGLQGEVD SLKSVKMELS SPTGSNVSQE 540 DGGLKDLVLD VKIMGSDAMI RVQSSSKNHP AAILMTALQQ LNLELQHASV SVVNNDLMIQ 600 QVTIKMTSPC YTQEQLKLAL SARFRFSS*

Sequence ? help Back to Top

Protein Sequence Length: 629 aa Download sequence Send to blast

MVNLWIDDAF QYDLSAFWPL PSSSSTTAHL DPPNPQLQNN PFTQESLHQR LLALLDTTPT  60
QKWTYAIFWQ LSHSSTLGWG DGYYKGDDTK GQRRASDSTW LAEQDNRKKI LRELNSIISG  120
PGSVSLDDAV DEEVTDTEWF FLISMTQSFV NGDGLPGQAC LCSSPVWVVG EDLSSSSCER  180
ARQSRVFGIQ TMVCIPAVNG VVELGSTDLI FQSSDLMSRV RSLFHFEGFE SGNPWPVLSL  240
TGQDESGYDD QLAMLLTFSE PAQSVEMRDS GNGVGSSNHL AAAAAAAAAS SNVHVTNGNP  300
SAASCVTENA GYKVDIPIGT PNLLQGQNFG SAGNPDLHWV RPKSGENLSF GEILKTSSSC  360
GGLVPDDDFA QFVASGGDGD KRKKSAASRQ RKEEGILSFT PPAGGRVLKP AVAVKCGEHY  420
EGSVKEADCS RVVEPEKKQP KKRGRKPANG RDEPINHVEA ERLRREKLNQ KFYALRAVVP  480
TVSKMDKASL LGDAISYING LTAKLESLEA DVQGLQGEVD SLKSVKMELS SPTGSNVSQE  540
DGGLKDLVLD VKIMGSDAMI RVQSSSKNHP AAILMTALQQ LNLELQHASV SVVNNDLMIQ  600
QVTIKMTSPC YTQEQLKLAL SARFRFSS*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4rqw_A	4e-60	42	226	4	194	Transcription factor MYC3
4rqw_B	4e-60	42	226	4	194	Transcription factor MYC3
4rs9_A	4e-60	42	226	4	194	Transcription factor MYC3
4yz6_A	4e-60	42	226	4	194	Transcription factor MYC3
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Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator that binds to the G-box motif (5'-AACGTG-3') found in the promoter of the jasmonate-induced gene LAPA1 (PubMed:15231736). Acts as negative regulator of blue light-mediated photomorphogenesis and positively regulates root growth (PubMed:24483714). Promotes growth in response to the phytohormones abscisic acid (ABA) and jasmonate (JA) (PubMed:24483714). Binds to the G-box motif (5'-CACGTG-3') of the RBCS-3A gene promoter (PubMed:24483714). Acts downstream of the jasmonate (JA) receptor to orchestrate JA-mediated activation of plant responses (PubMed:28733419). Positively regulates both wound-responsive and pathogen-responsive genes through MYC2-targeted transcription factors (MTFs) involved in early response to JA (PubMed:28733419). With JA2L forms a transcription module that regulates wounding-responsive genes (PubMed:28733419). With ERF.C3 forms a transcription module that regulates pathogen-responsive genes (PubMed:28733419). Plays a critical role in orchestrating JA-mediated defense gene expression during Botrytis cinerea infection (PubMed:28733419). Regulates negatively defense responses to root-knot nematodes, potentially by mediating crosstalk among the hormones strigolactones, abscisic acid (ABA) and jasmonate (JA) (PubMed:30576511). Regulates the termination of JA-mediated defense responses by specifically binding the G-box (5'-CACATG-3') motifs in the promoters of MTB1, MTB2 and MTB3, which are transcription factors that negatively regulates JA signaling (PubMed:30610166). May be involved in JA-induced chilling tolerance, possibly by ameliorating the antioxidant enzyme system of fruit and increasing proline and lycopene levels (PubMed:29528226). {ECO:0000269\|PubMed:15231736, ECO:0000269\|PubMed:24483714, ECO:0000269\|PubMed:28733419, ECO:0000269\|PubMed:29528226, ECO:0000269\|PubMed:30576511, ECO:0000269\|PubMed:30610166}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by methyl jasmonate (PubMed:15231736, PubMed:28733419). Induced by wounding (PubMed:15231736). Induced by infection with the fungal pathogen Botrytis cinerea (PubMed:28733419). Induced in fruit by storage in cold (PubMed:29528226). Induced by hydrogen peroxide and infection with root-knot nematodes (PubMed:30576511). {ECO:0000269\|PubMed:15231736, ECO:0000269\|PubMed:28733419, ECO:0000269\|PubMed:29528226, ECO:0000269\|PubMed:30576511}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_002280253.2	0.0	PREDICTED: transcription factor MYC2
Swissprot	A0A3Q7HRZ6	1e-173	MYC2_SOLLC; Transcription factor MYC2
TrEMBL	F6HT90	0.0	F6HT90_VITVI; Uncharacterized protein
STRING	VIT_02s0012g01320.t01	0.0	(Vitis vinifera)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G32640.1	1e-117	bHLH family protein

Link Out ? help Back to Top
Phytozome	Kalax.0967s0002.1.p

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Gupta N,Prasad VB,Chattopadhyay S LeMYC2 acts as a negative regulator of blue light mediated photomorphogenic growth, and promotes the growth of adult tomato plants. BMC Plant Biol., 2014. 14: p. 38 [PMID:24483714] Sun H,Fan HJ,Ling HQ Genome-wide identification and characterization of the bHLH gene family in tomato. BMC Genomics, 2015. 16: p. 9 [PMID:25612924] Du M, et al. MYC2 Orchestrates a Hierarchical Transcriptional Cascade That Regulates Jasmonate-Mediated Plant Immunity in Tomato. Plant Cell, 2017. 29(8): p. 1883-1906 [PMID:28733419] Min D, et al. SlMYC2 Involved in Methyl Jasmonate-Induced Tomato Fruit Chilling Tolerance. J. Agric. Food Chem., 2018. 66(12): p. 3110-3117 [PMID:29528226] Xu X, et al. Strigolactones positively regulate defense against root-knot nematodes in tomato. J. Exp. Bot., 2019. 70(4): p. 1325-1337 [PMID:30576511] Liu Y, et al. MYC2 Regulates the Termination of Jasmonate Signaling via an Autoregulatory Negative Feedback Loop. Plant Cell, 2019. 31(1): p. 106-127 [PMID:30610166]