PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; Saxifragales; Crassulaceae; Kalanchoe

Family

bHLH

Protein Properties

Length: 572aa MW: 61398.6 Da PI: 5.159

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Kalax.0347s0049.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	35.7	1.5e-11	387	429	7	54
HHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHH CS HLH 7 erErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksL 54 ++ErrRR+++N+++ Lr+++Pk + K++ a+iL A+eY+k+L Kalax.0347s0049.1.p 387 MAERRRRKKLNDRLYMLRSVVPKiS------KMDRASILGDAIEYLKEL 429 79*******************66......**************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	15.58	380	429	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.57E-16	383	449	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	4.4E-14	386	435	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	3.5E-9	386	429	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	5.1E-16	387	442	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	5.75E-13	387	433	No hit	No description
CDD	cd04873	3.19E-6	500	541	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 572 aa Download sequence Send to blast
MISGVNVWMG DREEDQNPGG WGAGTNGCLN LGAPTEQRVG GEEDDINSSS LSAFKSMLEV 60 EEEDDWYNNG GAAAVAGGLH PHIAYASNPD ALGAAATAAG DGHNITNSLM LYQQQQHHSV 120 DSSASGSPSF NNLDASQVQF YLQPKPMPSS SSLLSFMPAQ LDHPSFDLGC EQNFLDAQSS 180 SFFMNANGRG SGVFFNGFSD LMNSNSNHQI SNLMGLGSRF SIDNNGVGTG SGVGFDLGFK 240 GFDDVGSNGA FMNRSKLLRP LDSFPSTGAQ PTLFQKRAAL RKNLAESGGG NVGVLALQSG 300 EVEIDKGKRE MGLNEMLLKR KSRSGMDEFD DVSIDGSGLN YDSDEFMESY KLEENGKNGG 360 SSSNANSTVT GGDHKGKKKG LPAKNLMAER RRRKKLNDRL YMLRSVVPKI SKMDRASILG 420 DAIEYLKELL QKINDLHNEL ESTPAGSSLT PTTPFPPLTP TTSTLPCRIK EELCPSSLPS 480 PDGQPARVEV RLREGRSVNI HMFCSRRPGL LLSTMRALDT LGLDIQQAVI SCFNGFAMDI 540 FKAEQCKEGQ DVHPDQIKAM LLESAGFHGV A*

Sequence ? help Back to Top

Protein Sequence Length: 572 aa Download sequence Send to blast

MISGVNVWMG DREEDQNPGG WGAGTNGCLN LGAPTEQRVG GEEDDINSSS LSAFKSMLEV  60
EEEDDWYNNG GAAAVAGGLH PHIAYASNPD ALGAAATAAG DGHNITNSLM LYQQQQHHSV  120
DSSASGSPSF NNLDASQVQF YLQPKPMPSS SSLLSFMPAQ LDHPSFDLGC EQNFLDAQSS  180
SFFMNANGRG SGVFFNGFSD LMNSNSNHQI SNLMGLGSRF SIDNNGVGTG SGVGFDLGFK  240
GFDDVGSNGA FMNRSKLLRP LDSFPSTGAQ PTLFQKRAAL RKNLAESGGG NVGVLALQSG  300
EVEIDKGKRE MGLNEMLLKR KSRSGMDEFD DVSIDGSGLN YDSDEFMESY KLEENGKNGG  360
SSSNANSTVT GGDHKGKKKG LPAKNLMAER RRRKKLNDRL YMLRSVVPKI SKMDRASILG  420
DAIEYLKELL QKINDLHNEL ESTPAGSSLT PTTPFPPLTP TTSTLPCRIK EELCPSSLPS  480
PDGQPARVEV RLREGRSVNI HMFCSRRPGL LLSTMRALDT LGLDIQQAVI SCFNGFAMDI  540
FKAEQCKEGQ DVHPDQIKAM LLESAGFHGV A*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5gnj_A	5e-14	382	439	6	63	Transcription factor MYC2
5gnj_B	5e-14	382	439	6	63	Transcription factor MYC2
5gnj_E	5e-14	382	439	6	63	Transcription factor MYC2
5gnj_F	5e-14	382	439	6	63	Transcription factor MYC2
5gnj_G	5e-14	382	439	6	63	Transcription factor MYC2
5gnj_I	5e-14	382	439	6	63	Transcription factor MYC2
5gnj_M	5e-14	382	439	6	63	Transcription factor MYC2
5gnj_N	5e-14	382	439	6	63	Transcription factor MYC2
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	376	394	KKKGLPAKNLMAERRRRKK
2	388	395	ERRRRKKL

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. Functions as a dimer with SPCH during stomatal initiation (PubMed:18641265, PubMed:28507175). {ECO:0000269\|PubMed:17416732, ECO:0000269\|PubMed:18641265, ECO:0000269\|PubMed:28507175}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By high-salt stress, cold stress and abscisic acid (ABA) treatment.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_012489049.1	0.0	PREDICTED: transcription factor ICE1-like
Refseq	XP_023877763.1	0.0	transcription factor ICE1-like
Swissprot	Q9LSE2	1e-123	ICE1_ARATH; Transcription factor ICE1
TrEMBL	A0A220BF34	0.0	A0A220BF34_SANAL; ICE1
STRING	Gorai.007G044800.1	0.0	(Gossypium raimondii)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G26744.4	1e-116	bHLH family protein

Link Out ? help Back to Top
Phytozome	Kalax.0347s0049.1.p

Publications ? help Back to Top
Chen Y, et al. Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398. BMC Biol., 2013. 11: p. 121 [PMID:24350981] Xu F, et al. Increased drought tolerance through the suppression of ESKMO1 gene and overexpression of CBF-related genes in Arabidopsis. PLoS ONE, 2014. 9(9): p. e106509 [PMID:25184213] Jiang W,Wu J,Zhang Y,Yin L,Lu J Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses. Protoplasma, 2015. 252(5): p. 1361-74 [PMID:25643917] Lang Z,Zhu J OST1 phosphorylates ICE1 to enhance plant cold tolerance. Sci China Life Sci, 2015. 58(3): p. 317-8 [PMID:25680856] Juan JX, et al. Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene. Genet. Mol. Res., 2015. 14(1): p. 597-608 [PMID:25729995] Lee HG,Seo PJ The MYB96-HHP module integrates cold and abscisic acid signaling to activate the CBF-COR pathway in Arabidopsis. Plant J., 2015. 82(6): p. 962-77 [PMID:25912720] Horst RJ, et al. Molecular Framework of a Regulatory Circuit Initiating Two-Dimensional Spatial Patterning of Stomatal Lineage. PLoS Genet., 2015. 11(7): p. e1005374 [PMID:26203655] Lee JH,Jung JH,Park CM INDUCER OF CBF EXPRESSION 1 integrates cold signals into FLOWERING LOCUS C-mediated flowering pathways in Arabidopsis. Plant J., 2015. 84(1): p. 29-40 [PMID:26248809] Wang CL,Zhang SC,Qi SD,Zheng CC,Wu CA Delayed germination of Arabidopsis seeds under chilling stress by overexpressing an abiotic stress inducible GhTPS11. Gene, 2016. 575(2 Pt 1): p. 206-12 [PMID:26325072] Lee JH,Park CM Integration of photoperiod and cold temperature signals into flowering genetic pathways in Arabidopsis. Plant Signal Behav, 2015. 10(11): p. e1089373 [PMID:26430754] Su F, et al. Burkholderia phytofirmans PsJN reduces impact of freezing temperatures on photosynthesis in Arabidopsis thaliana. Front Plant Sci, 2015. 6: p. 810 [PMID:26483823] Klermund C, et al. LLM-Domain B-GATA Transcription Factors Promote Stomatal Development Downstream of Light Signaling Pathways in Arabidopsis thaliana Hypocotyls. Plant Cell, 2016. 28(3): p. 646-60 [PMID:26917680] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lu X, et al. A novel Zea mays ssp. mexicana L. MYC-type ICE-like transcription factor gene ZmmICE1, enhances freezing tolerance in transgenic Arabidopsis thaliana. Plant Physiol. Biochem., 2017. 113: p. 78-88 [PMID:28189052] Deng C,Ye H,Fan M,Pu T,Yan J The rice transcription factors OsICE confer enhanced cold tolerance in transgenic Arabidopsis. Plant Signal Behav, 2017. 12(5): p. e1316442 [PMID:28414264] de Marcos A, et al. A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development. Plant Physiol., 2017. 174(2): p. 823-842 [PMID:28507175] Kim SH, et al. Phosphorylation of the transcriptional repressor MYB15 by mitogen-activated protein kinase 6 is required for freezing tolerance in Arabidopsis. Nucleic Acids Res., 2017. 45(11): p. 6613-6627 [PMID:28510716] Pal S, et al. TransDetect Identifies a New Regulatory Module Controlling Phosphate Accumulation. Plant Physiol., 2017. 175(2): p. 916-926 [PMID:28827455] Zhao C, et al. MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability. Dev. Cell, 2017. 43(5): p. 618-629.e5 [PMID:29056551] Li H, et al. MPK3- and MPK6-Mediated ICE1 Phosphorylation Negatively Regulates ICE1 Stability and Freezing Tolerance in Arabidopsis. Dev. Cell, 2017. 43(5): p. 630-642.e4 [PMID:29056553] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Liu Y,Zhou J MAPping Kinase Regulation of ICE1 in Freezing Tolerance. Trends Plant Sci., 2018. 23(2): p. 91-93 [PMID:29248419] Xie H, et al. Variation in ICE1 Methylation Primarily Determines Phenotypic Variation in Freezing Tolerance in Arabidopsis thaliana. Plant Cell Physiol., 2019. 60(1): p. 152-165 [PMID:30295898]