PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; Saxifragales; Crassulaceae; Kalanchoe

Family

HSF

Protein Properties

Length: 393aa MW: 44192.4 Da PI: 4.586

Description

HSF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Kalax.0036s0098.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HSF_DNA-bind	114.1	9.2e-36	50	141	2	102
HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXX CS HSF_DNA-bind 2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkk 91 Fl+k+ye++ed++++e++sws+ nsfvv+d+++f++++Lp+yFkhsnf+SF+RQLn+YgF+kv+ ++ weF+++ F g+k Kalax.0036s0098.1.p 50 FLTKTYEMVEDPATDEVVSWSRARNSFVVWDSNKFSTTLLPRYFKHSNFSSFIRQLNTYGFRKVDPDQ---------WEFANEGFLGGQK 130 9**************************************************************999.........********* PP XXXXXXXXXXX CS HSF_DNA-bind 92 ellekikrkks 102 +ll++i+r+++ Kalax.0036s0098.1.p 131 HLLKTIRRRRH 141 ******986 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF46785	2.18E-34	46	139	IPR011991	Winged helix-turn-helix DNA-binding domain
Gene3D	G3DSA:1.10.10.10	3.3E-38	46	139	IPR011991	Winged helix-turn-helix DNA-binding domain
SMART	SM00415	1.8E-60	46	139	IPR000232	Heat shock factor (HSF)-type, DNA-binding
Pfam	PF00447	1.7E-31	50	139	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	1.6E-19	50	73	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	1.6E-19	88	100	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PROSITE pattern	PS00434	0	89	113	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	1.6E-19	101	113	IPR000232	Heat shock factor (HSF)-type, DNA-binding

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 393 aa Download sequence Send to blast
MEDAKTMIQE EEVVVIKSTT GMASAASSTT TSSVSPRPVE GLHDLGPPPF LTKTYEMVED 60 PATDEVVSWS RARNSFVVWD SNKFSTTLLP RYFKHSNFSS FIRQLNTYGF RKVDPDQWEF 120 ANEGFLGGQK HLLKTIRRRR HTAQNVQQQG GSSVCVEVGQ YGLEGELERL KRDRSVLMTE 180 VLRLKQQQHE SREYIVAMQN RLRGTERKQQ QMMGFLAKAF NNPTFIQQFK LETAERKALD 240 GANIKRKRRL TASTSQEILN KQIDILDVLE NSAGGGQGQE DQIDLINMES EIETLFAAGL 300 ESESSNDVKL PRKTESMAKA GGSNLDSVNE MLWDELLSDD VAVGGEDDEQ LFLEDIPDID 360 VPVEELGDAS PSDWSEDLKD LVEQMNYLKS KP*

Sequence ? help Back to Top

Protein Sequence Length: 393 aa Download sequence Send to blast

MEDAKTMIQE EEVVVIKSTT GMASAASSTT TSSVSPRPVE GLHDLGPPPF LTKTYEMVED  60
PATDEVVSWS RARNSFVVWD SNKFSTTLLP RYFKHSNFSS FIRQLNTYGF RKVDPDQWEF  120
ANEGFLGGQK HLLKTIRRRR HTAQNVQQQG GSSVCVEVGQ YGLEGELERL KRDRSVLMTE  180
VLRLKQQQHE SREYIVAMQN RLRGTERKQQ QMMGFLAKAF NNPTFIQQFK LETAERKALD  240
GANIKRKRRL TASTSQEILN KQIDILDVLE NSAGGGQGQE DQIDLINMES EIETLFAAGL  300
ESESSNDVKL PRKTESMAKA GGSNLDSVNE MLWDELLSDD VAVGGEDDEQ LFLEDIPDID  360
VPVEELGDAS PSDWSEDLKD LVEQMNYLKS KP*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5d5u_B	1e-24	48	139	27	129	Heat shock factor protein 1
5d5v_B	1e-24	48	139	27	129	Heat shock factor protein 1
5d5v_D	1e-24	48	139	27	129	Heat shock factor protein 1
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Functional Description ? help Back to Top
Source	Description
UniProt	DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. {ECO:0000250}.
UniProt	Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Involved in heat stress responses. Seems to be involved in other environmental stress responses. Activates ascorbate peroxidase 2 (APX2) in addition to several heat shock protein (HSPs). {ECO:0000269\|PubMed:16649111, ECO:0000269\|PubMed:17059409, ECO:0000269\|PubMed:17085506}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By heat stress, high light and hydrogen peroxide (H(2)O(2)). {ECO:0000269\|PubMed:16649111, ECO:0000269\|PubMed:17059409, ECO:0000269\|PubMed:17085506}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020536555.1	1e-160	heat shock factor protein HSF30
Swissprot	O80982	1e-127	HSFA2_ARATH; Heat stress transcription factor A-2
Swissprot	P41152	1e-127	HSF30_SOLPE; Heat shock factor protein HSF30
TrEMBL	A0A067KQI1	1e-158	A0A067KQI1_JATCU; Uncharacterized protein
STRING	VIT_04s0008g01110.t01	1e-151	(Vitis vinifera)
STRING	EOY29928	1e-151	(Theobroma cacao)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G26150.1	1e-129	heat shock transcription factor A2

Link Out ? help Back to Top
Phytozome	Kalax.0036s0098.1.p

Publications ? help Back to Top
Liu HC,Charng YY Common and distinct functions of Arabidopsis class A1 and A2 heat shock factors in diverse abiotic stress responses and development. Plant Physiol., 2013. 163(1): p. 276-90 [PMID:23832625] Jung HS, et al. Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light. Proc. Natl. Acad. Sci. U.S.A., 2013. 110(35): p. 14474-9 [PMID:23918368] Chauhan H,Khurana N,Agarwal P,Khurana JP,Khurana P A seed preferential heat shock transcription factor from wheat provides abiotic stress tolerance and yield enhancement in transgenic Arabidopsis under heat stress environment. PLoS ONE, 2013. 8(11): p. e79577 [PMID:24265778] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Weng M, et al. Histone chaperone ASF1 is involved in gene transcription activation in response to heat stress in Arabidopsis thaliana. Plant Cell Environ., 2014. 37(9): p. 2128-38 [PMID:24548003] Jang YH, et al. A homolog of splicing factor SF1 is essential for development and is involved in the alternative splicing of pre-mRNA in Arabidopsis thaliana. Plant J., 2014. 78(4): p. 591-603 [PMID:24580679] Stief A, et al. Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors. Plant Cell, 2014. 26(4): p. 1792-1807 [PMID:24769482] Dobrá J, et al. The impact of heat stress targeting on the hormonal and transcriptomic response in Arabidopsis. Plant Sci., 2015. 231: p. 52-61 [PMID:25575991] Yamauchi Y,Kunishima M,Mizutani M,Sugimoto Y Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression. Sci Rep, 2015. 5: p. 8030 [PMID:25619826] Shi H, et al. Melatonin induces class A1 heat-shock factors (HSFA1s) and their possible involvement of thermotolerance in Arabidopsis. J. Pineal Res., 2015. 58(3): p. 335-42 [PMID:25711624] Mueller SP,Krause DM,Mueller MJ,Fekete A Accumulation of extra-chloroplastic triacylglycerols in Arabidopsis seedlings during heat acclimation. J. Exp. Bot., 2015. 66(15): p. 4517-26 [PMID:25977236] Nie S,Yue H,Xing D A Potential Role for Mitochondrial Produced Reactive Oxygen Species in Salicylic Acid-Mediated Plant Acquired Thermotolerance. Plant Physiol., 2016. [PMID:26099269] Nguyen AH, et al. Loss of Arabidopsis 5'-3' Exoribonuclease AtXRN4 Function Enhances Heat Stress Tolerance of Plants Subjected to Severe Heat Stress. Plant Cell Physiol., 2015. 56(9): p. 1762-72 [PMID:26136597] Hu Z, et al. Histone acetyltransferase GCN5 is essential for heat stress-responsive gene activation and thermotolerance in Arabidopsis. Plant J., 2015. 84(6): p. 1178-91 [PMID:26576681] Lämke J,Brzezinka K,Bäurle I HSFA2 orchestrates transcriptional dynamics after heat stress in Arabidopsis thaliana. Transcription, 2016. 7(4): p. 111-4 [PMID:27383578] Wang X,Huang W,Liu J,Yang Z,Huang B Molecular regulation and physiological functions of a novel FaHsfA2c cloned from tall fescue conferring plant tolerance to heat stress. Plant Biotechnol. J., 2017. 15(2): p. 237-248 [PMID:27500592] Chen ST,He NY,Chen JH,Guo FQ Identification of core subunits of photosystem II as action sites of HSP21, which is activated by the GUN5-mediated retrograde pathway in Arabidopsis. Plant J., 2017. 89(6): p. 1106-1118 [PMID:27943531] Kataoka R,Takahashi M,Suzuki N Coordination between bZIP28 and HSFA2 in the regulation of heat response signals in Arabidopsis. Plant Signal Behav, 2017. 12(11): p. e1376159 [PMID:28873003] Wang X,Zhuang L,Shi Y,Huang B Up-Regulation of HSFA2c and HSPs by ABA Contributing to Improved Heat Tolerance in Tall Fescue and Arabidopsis. Int J Mol Sci, 2018. [PMID:28914758] Llamas E,Pulido P,Rodriguez-Concepcion M Interference with plastome gene expression and Clp protease activity in Arabidopsis triggers a chloroplast unfolded protein response to restore protein homeostasis. PLoS Genet., 2017. 13(9): p. e1007022 [PMID:28937985] Li X, et al. Plastid Translation Elongation Factor Tu Is Prone to Heat-Induced Aggregation Despite Its Critical Role in Plant Heat Tolerance. Plant Physiol., 2018. 176(4): p. 3027-3045 [PMID:29444814] Scharf KD,Rose S,Thierfelder J,Nover L Two cDNAs for tomato heat stress transcription factors. Plant Physiol., 1993. 102(4): p. 1355-6 [PMID:8278556]