PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza

Family

WRKY

Protein Properties

Length: 185aa MW: 20442.4 Da PI: 9.8936

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
KN539326.1_FGP004	genome	BGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	99.7	1.9e-31	106	164	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 D ++WrKYGqK++kgs+fpr+YY+C++ gCp++k+ver+ +dp+++++tYegeH+h+ KN539326.1_FGP004 106 ADDFSWRKYGQKPIKGSPFPRGYYKCSTLrGCPARKHVERDPTDPSMLIVTYEGEHRHT 164 699*********************9988**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF10533	1.5E-9	84	103	IPR018872	Zn-cluster domain
Gene3D	G3DSA:2.20.25.80	1.5E-32	93	164	IPR003657	WRKY domain
PROSITE profile	PS50811	29.792	100	166	IPR003657	WRKY domain
SuperFamily	SSF118290	3.01E-26	103	164	IPR003657	WRKY domain
SMART	SM00774	7.4E-37	105	165	IPR003657	WRKY domain
Pfam	PF03106	1.7E-26	107	163	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009737	Biological Process	response to abscisic acid
GO:0009938	Biological Process	negative regulation of gibberellic acid mediated signaling pathway
GO:0010200	Biological Process	response to chitin
GO:0042742	Biological Process	defense response to bacterium
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005516	Molecular Function	calmodulin binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:1990841	Molecular Function	promoter-specific chromatin binding

Sequence ? help Back to Top
Protein Sequence Length: 185 aa Download sequence Send to blast
MDLMSGYGRV DEQVAIQEAA AAGLRGMEHL ILQLSQTGTS ERSPAPAPAQ EQQQQQVDCR 60 EITDMTVSKF KKVISMLNRT GHARKHRVKR TIRVPAISSK VADIPADDFS WRKYGQKPIK 120 GSPFPRGYYK CSTLRGCPAR KHVERDPTDP SMLIVTYEGE HRHTPSAAGQ DHPPAPPPPL 180 ALPLA

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	2e-21	93	163	2	71	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts, when in complex with WRKY71, specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Represses specifically gibberellic acid (GA)-induced promoters in aleurone cells, probably by interfering with GAM1. {ECO:0000269\|PubMed:16623886}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00464	DAP	Transfer from AT4G31550	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	KN539326.1_FGP004

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by abscisic acid (ABA) in aleurone cells (PubMed:15618416, PubMed:16623886). Accumulates in response to uniconazole, a gibberellic acid (GA) biosynthesis inhibitor (PubMed:16623886). Repressed by GA (PubMed:15618416). {ECO:0000269\|PubMed:15618416, ECO:0000269\|PubMed:16623886}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK100954	1e-167	AK100954.1 Oryza sativa Japonica Group cDNA clone:J023139N16, full insert sequence.
GenBank	AK121494	1e-167	AK121494.1 Oryza sativa Japonica Group cDNA clone:J033000L17, full insert sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_015633572.1	6e-58	WRKY transcription factor WRKY51
Swissprot	Q0JEE2	5e-59	WRK51_ORYSJ; WRKY transcription factor WRKY51
TrEMBL	A0A0E0DC00	4e-61	A0A0E0DC00_9ORYZ; Uncharacterized protein
STRING	OMERI04G19410.1	1e-69	(Oryza meridionalis)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP617	38	171

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G31550.1	1e-47	WRKY DNA-binding protein 11

Publications ? help Back to Top
Kikuchi S, et al. Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice. Science, 2003. 301(5631): p. 376-9 [PMID:12869764] Zhang Y,Wang L The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol. Biol., 2005. 5: p. 1 [PMID:15629062] Wu KL,Guo ZJ,Wang HH,Li J The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res., 2005. 12(1): p. 9-26 [PMID:16106749] Xie Z, et al. Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells. Plant J., 2006. 46(2): p. 231-42 [PMID:16623886] Yilmaz A, et al. GRASSIUS: a platform for comparative regulatory genomics across the grasses. Plant Physiol., 2009. 149(1): p. 171-80 [PMID:18987217] Zhang ZL, et al. A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells. Plant Mol. Biol., 2009. 70(1-2): p. 139-51 [PMID:19199048] Hwang SH, et al. OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae. Plant Cell Rep., 2016. 35(9): p. 1975-85 [PMID:27300023]