PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Glycine; Soja

Family

WRKY

Protein Properties

Length: 619aa MW: 67583.4 Da PI: 6.6579

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
KHN23479.1	genome	TCUHK	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	97	1.3e-30	358	416	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 Dg++WrKYGqK+ kg+++pr+YYrCt+a gCpv+k+v+r+aed +++++tYeg+Hnh+ KHN23479.1 358 TDGCQWRKYGQKMAKGNPCPRAYYRCTMAaGCPVRKQVQRCAEDRTILITTYEGNHNHP 416 7*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.4E-33	342	418	IPR003657	WRKY domain
SuperFamily	SSF118290	1.19E-27	350	418	IPR003657	WRKY domain
PROSITE profile	PS50811	29.792	352	418	IPR003657	WRKY domain
SMART	SM00774	1.0E-36	357	417	IPR003657	WRKY domain
Pfam	PF03106	1.2E-26	359	416	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0016021	Cellular Component	integral component of membrane
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 619 aa Download sequence Send to blast
MARGGGLSID SDPIGSFLPH EPIVLNSFPE DNKDNNSRQQ HKWKLGTNMD ATVSRKSSPS 60 STSNTNTTIP FQDHLTSDDK MPHLDEMNFF PNKSNKDDDD DNNLASASTS TPPSLDHLHH 120 THDHSSAILG LKVNTGLNLL TTNASSDQSM VVDDDISPNS GDKRAKSEMV VLQVELERMK 180 VENHRLKNML DQVNNNYNAL QTHLVSLMKD QMDKEDDKQQ PHQVFDGKLE EKQAGNGGGA 240 LVPRQFMDLG LATNADTNET SHSHSSSVIR SQDSPPTNNT EVASKKNGGA SDEGLVFDQD 300 KKEFGRGIER EDSPSDQGVA ANNNVPKFSP PRNVDQAEAT MRKARVSVRA RSEAPMITDG 360 CQWRKYGQKM AKGNPCPRAY YRCTMAAGCP VRKQVQRCAE DRTILITTYE GNHNHPLPPA 420 AMAMAQTTSS AARMLLSGSM SSADGLMNAS FLTRTLLPCS SSMATISASA PFPTVTLDLT 480 QSPNPLQFPK QPSQFQIPFP GVPQNFANSQ ASLLPQIFGQ ALYNQSKFSG LQMSQDSDPS 540 QLSNQSQRPP PHLADTVSAA IAADPNFTAA LAAAITSIIG GAQPNNNSTT STNYNYIYIY 600 IYIYIYILKI LQIIKVIKK

Sequence ? help Back to Top

Protein Sequence Length: 619 aa Download sequence Send to blast

MARGGGLSID SDPIGSFLPH EPIVLNSFPE DNKDNNSRQQ HKWKLGTNMD ATVSRKSSPS  60
STSNTNTTIP FQDHLTSDDK MPHLDEMNFF PNKSNKDDDD DNNLASASTS TPPSLDHLHH  120
THDHSSAILG LKVNTGLNLL TTNASSDQSM VVDDDISPNS GDKRAKSEMV VLQVELERMK  180
VENHRLKNML DQVNNNYNAL QTHLVSLMKD QMDKEDDKQQ PHQVFDGKLE EKQAGNGGGA  240
LVPRQFMDLG LATNADTNET SHSHSSSVIR SQDSPPTNNT EVASKKNGGA SDEGLVFDQD  300
KKEFGRGIER EDSPSDQGVA ANNNVPKFSP PRNVDQAEAT MRKARVSVRA RSEAPMITDG  360
CQWRKYGQKM AKGNPCPRAY YRCTMAAGCP VRKQVQRCAE DRTILITTYE GNHNHPLPPA  420
AMAMAQTTSS AARMLLSGSM SSADGLMNAS FLTRTLLPCS SSMATISASA PFPTVTLDLT  480
QSPNPLQFPK QPSQFQIPFP GVPQNFANSQ ASLLPQIFGQ ALYNQSKFSG LQMSQDSDPS  540
QLSNQSQRPP PHLADTVSAA IAADPNFTAA LAAAITSIIG GAQPNNNSTT STNYNYIYIY  600
IYIYIYILKI LQIIKVIKK

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	6e-22	344	420	1	76	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269\|PubMed:12000796, ECO:0000269\|PubMed:25733771}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	KHN23479.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	EU019575	0.0	EU019575.1 Glycine max WRKY36 (WRKY36) mRNA, partial cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_028191238.1	0.0	probable WRKY transcription factor 31
Swissprot	Q9C519	1e-127	WRKY6_ARATH; WRKY transcription factor 6
TrEMBL	A0A0B2QTR9	0.0	A0A0B2QTR9_GLYSO; WRKY transcription factor 6
STRING	GLYMA13G38630.1	0.0	(Glycine max)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF866	34	121

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G62300.1	1e-116	WRKY family protein

Publications ? help Back to Top
Zhou F, et al. High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J., 2004. 39(6): p. 920-32 [PMID:15341634] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Qi X, et al. Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing. Nat Commun, 2014. 5: p. 4340 [PMID:25004933] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Chai J,Liu J,Zhou J,Xing D Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid. J. Exp. Bot., 2014. 65(22): p. 6513-28 [PMID:25210078] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Xiao D, et al. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. Plant Physiol., 2015. 169(2): p. 1275-91 [PMID:26304848] Bakshi M, et al. WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis. BMC Plant Biol., 2015. 15: p. 305 [PMID:26718529] Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development. PLoS Genet., 2016. 12(2): p. e1005833 [PMID:26829043] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Li H, et al. BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis. Mol Plant, 2017. 10(4): p. 545-559 [PMID:28089951] Stetter MG,Benz M,Ludewig U Increased root hair density by loss of WRKY6 in Arabidopsis thaliana. PeerJ, 2017. 5: p. e2891 [PMID:28149680] Zhou X, et al. StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato. J. Exp. Bot., 2017. 68(5): p. 1265-1281 [PMID:28338870] Bakshi M, et al. Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation. Front Microbiol, 2017. 8: p. 1262 [PMID:28747898] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486]