PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis

Family

MYB

Protein Properties

Length: 287aa MW: 32610.5 Da PI: 6.6876

Description

MYB family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
KFK35251.1	genome	MPIPBR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	Myb_DNA-binding	42.1	1.9e-13	14	59	1	46
TSSS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHHHH CS Myb_DNA-binding 1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqk 46 +g+W +eEde l++ v+++G+++W++I ++ ++Rt+k+c++rw + KFK35251.1 14 KGPWKAEEDEVLLNHVNRYGPRDWSSIRSKGLLHRTGKSCRLRWVN 59 79***********************888779*********87 PP
2	Myb_DNA-binding	43.3	8.6e-14	70	109	3	44
SS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHH CS Myb_DNA-binding 3 rWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrw 44 ++++eE+ + +++ ++G++ W++Ia +++ gRt++++k++w KFK35251.1 70 KFSAEEERIVIELQSEFGNK-WARIATYLP-GRTDNDVKNFW 109 79****************.*****.********* PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:1.10.10.60	3.6E-21	8	62	IPR009057	Homeodomain-like
PROSITE profile	PS51294	21.283	9	65	IPR017930	Myb domain
SuperFamily	SSF46689	1.22E-28	12	109	IPR009057	Homeodomain-like
SMART	SM00717	6.7E-12	13	63	IPR001005	SANT/Myb domain
CDD	cd00167	4.02E-11	16	61	No hit	No description
Pfam	PF13921	1.6E-11	17	67	No hit	No description
Gene3D	G3DSA:1.10.10.60	1.9E-18	63	115	IPR009057	Homeodomain-like
PROSITE profile	PS51294	18.735	66	117	IPR017930	Myb domain
SMART	SM00717	5.3E-13	67	115	IPR001005	SANT/Myb domain
Pfam	PF00249	1.5E-12	70	109	IPR001005	SANT/Myb domain
CDD	cd00167	1.60E-10	70	109	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0048235	Biological Process	pollen sperm cell differentiation
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 287 aa Download sequence Send to blast
MKKTESTKKE EIKKGPWKAE EDEVLLNHVN RYGPRDWSSI RSKGLLHRTG KSCRLRWVNK 60 LRPNLKNGCK FSAEEERIVI ELQSEFGNKW ARIATYLPGR TDNDVKNFWS SRQKRLARIL 120 HNSSDASSSS NLNPKSSSSS SQNKGKNVKP IQSFNPNSQG FGLLEEEVIT ATETVDESLR 180 LPELGVKLEH QPFAFGTDLV SEVQIDANDQ QGMIPFSPES RELLARLDDP YFYDILGPAD 240 SSVEPLFGLP QPFFEPSPRR CRHGSKDDFL DDFPADMFDQ VDPIRSP

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1h8a_C	4e-28	11	115	24	126	MYB TRANSFORMING PROTEIN
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Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator that acts as a positive regulator of male germline development by promoting both gametic cell specification and cell cycle progression (PubMed:15694308, PubMed:15618418, PubMed:19300502, PubMed:21285328). Binds to canonical MYB sites 5'-AACCGTC-3', 5'-AAACCGC-3' and 5'-AACCGT-3' in promoters to trigger the expression of male germline-specific or enriched genes (e.g. MGH3, GEX2 and GCS1), including those required for fertilization (PubMed:21285328, PubMed:19300502). Required for sperm cell specification leading to pollen maturation by activating a germline-specific regulon (PubMed:21285328, PubMed:15694308, PubMed:15618418, PubMed:19300502). Involved in pollen mitosis entry at G2-M transition via the regulation of CYCB1-1, DAZ1 and DAZ2 expression (PubMed:15618418, PubMed:19300502, PubMed:24876252). {ECO:0000269\|PubMed:15618418, ECO:0000269\|PubMed:15694308, ECO:0000269\|PubMed:19300502, ECO:0000269\|PubMed:21285328, ECO:0000269\|PubMed:24876252}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	KFK35251.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Repressed by the microRNA 159 (miR159); the production of miR159 is stimulated by the anaphase promoting complex/cyclosome (APC/C) (PubMed:21441434). Activated by ARID1 in male germline cells via specific histone acetylation regulation (e.g. H3K9Ac) (PubMed:25057814). {ECO:0000269\|PubMed:21441434, ECO:0000269\|PubMed:25057814}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF469468	1e-151	AF469468.1 Arabidopsis thaliana putative transcription factor (MYB125) mRNA, complete cds.
GenBank	HM776521	1e-151	HM776521.1 Arabidopsis thaliana duo pollen 1 (DUO1) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006402579.2	1e-157	transcription factor DUO1
Swissprot	A0A178VEK7	1e-150	DUO1_ARATH; Transcription factor DUO1
TrEMBL	A0A087GZE9	0.0	A0A087GZE9_ARAAL; Uncharacterized protein
STRING	A0A087GZE9	0.0	(Arabis alpina)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM6551	28	42

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G60460.1	1e-137	MYB family protein

Publications ? help Back to Top
Zheng B,He H,Zheng Y,Wu W,McCormick S An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thaliana. PLoS Genet., 2014. 10(7): p. e1004421 [PMID:25057814] Peters B, et al. A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter. Plant Physiol., 2017. 173(1): p. 280-293 [PMID:27624837] Zheng Z, et al. Target RNA Secondary Structure Is a Major Determinant of miR159 Efficacy. Plant Physiol., 2017. 174(3): p. 1764-1778 [PMID:28515145]