PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis

Family

WRKY

Protein Properties

Length: 321aa MW: 35541.3 Da PI: 10.2505

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
KFK29738.1	genome	MPIPBR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	97.5	8.5e-31	243	300	3	59
-SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 3 DgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 D+++WrKYGqK++kgs++pr+YY+C++ gCp++k+ver+ +dp+++++tYegeH+h+ KFK29738.1 243 DEFSWRKYGQKPIKGSPHPRGYYKCSTFrGCPARKHVERALDDPTMLIVTYEGEHRHN 300 99*********************8766**************************8 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF10533	2.5E-17	191	239	IPR018872	Zn-cluster domain
Gene3D	G3DSA:2.20.25.80	3.0E-32	228	301	IPR003657	WRKY domain
PROSITE profile	PS50811	29.838	236	302	IPR003657	WRKY domain
SuperFamily	SSF118290	2.48E-26	239	301	IPR003657	WRKY domain
SMART	SM00774	3.3E-37	241	301	IPR003657	WRKY domain
Pfam	PF03106	1.6E-26	243	299	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009737	Biological Process	response to abscisic acid
GO:0009938	Biological Process	negative regulation of gibberellic acid mediated signaling pathway
GO:0010200	Biological Process	response to chitin
GO:0042742	Biological Process	defense response to bacterium
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005516	Molecular Function	calmodulin binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:1990841	Molecular Function	promoter-specific chromatin binding

Sequence ? help Back to Top
Protein Sequence Length: 321 aa Download sequence Send to blast
MAVDLMRFPK IDDQKAIQEA ASQGIQSMEH LIRLLSNRPE QHNNVDCSEI TDFTVSKFKT 60 VISLLNRTGH ARFRRGPVHS PLPPPQIVKI AQSEAPVTRK PTNLVPPPSS TFVHSNQPSV 120 TLDFTKPSIF GSKSKSSELE FAKENFSVSL NSSYISSAIT GDGSVSKGSS IFHASQPVTS 180 SGKPPLAHPY RKRCLEHEHS EDFSGKISGS GHGKCHCKKR KNRMKRTVRV PAISTKIADI 240 PPDEFSWRKY GQKPIKGSPH PRGYYKCSTF RGCPARKHVE RALDDPTMLI VTYEGEHRHN 300 QSAMQENISS SGVNDLVFAS A

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	8e-20	229	300	2	72	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000, probably by activating the jasmonic acid (JA)- signaling pathway (PubMed:26433201). {ECO:0000250, ECO:0000269\|PubMed:26433201}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00464	DAP	Transfer from AT4G31550	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	KFK29738.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Strongly during leaf senescence (PubMed:11722756). Repressed by rhizobacterium B.cereus AR156 in leaves, and to a lower extent, by P.fluorescens WCS417r (PubMed:26433201). {ECO:0000269\|PubMed:11722756, ECO:0000269\|PubMed:26433201}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK352796	0.0	AK352796.1 Thellungiella halophila mRNA, complete cds, clone: RTFL01-05-N09.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_010432849.1	0.0	PREDICTED: probable WRKY transcription factor 11 isoform X2
Swissprot	Q9SV15	0.0	WRK11_ARATH; Probable WRKY transcription factor 11
TrEMBL	A0A087GIN6	0.0	A0A087GIN6_ARAAL; Uncharacterized protein
STRING	A0A087GIN7	0.0	(Arabis alpina)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G31550.2	0.0	WRKY DNA-binding protein 11

Publications ? help Back to Top
Haas BJ, et al. Full-length messenger RNA sequences greatly improve genome annotation. Genome Biol., 2002. 3(6): p. RESEARCH0029 [PMID:12093376] Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro. Plant Methods, 2010. 6: p. 25 [PMID:21108821] Brand LH, et al. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA. PLoS ONE, 2013. 8(10): p. e75177 [PMID:24146751] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Jiang CH, et al. Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. J. Exp. Bot., 2016. 67(1): p. 157-74 [PMID:26433201] Ali MA, et al. Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis. J. Plant Physiol., 2018. 226: p. 12-21 [PMID:29689430]