PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Arabideae; Arabis

Family

WRKY

Protein Properties

Length: 316aa MW: 35860.3 Da PI: 9.5977

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
KFK25104.1	genome	MPIPBR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	101.2	6.1e-32	128	186	2	60
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 +DgynWrKYGqK+vkgse+prsY++Ct+++C +kkkve+s +++++ei+Y+g+Hnh+k KFK25104.1 128 EDGYNWRKYGQKQVKGSENPRSYFKCTYPNCITKKKVETSLVNGHITEIIYKGSHNHPK 186 7********************************************************85 PP
2	WRKY	104.2	7.1e-33	248	305	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 +Dgy+WrKYGqK+vkg+++prsYY+Ct++gC+v+k+ver+ +d k v++tYeg+Hnh+ KFK25104.1 248 EDGYRWRKYGQKVVKGNPNPRSYYKCTFTGCCVRKHVERAFQDAKSVITTYEGKHNHQ 305 8********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	23.629	122	187	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	1.2E-26	122	187	IPR003657	WRKY domain
SuperFamily	SSF118290	4.32E-25	124	187	IPR003657	WRKY domain
SMART	SM00774	4.1E-33	127	186	IPR003657	WRKY domain
Pfam	PF03106	4.9E-23	128	185	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	4.2E-36	232	307	IPR003657	WRKY domain
SuperFamily	SSF118290	1.83E-28	239	307	IPR003657	WRKY domain
PROSITE profile	PS50811	36.013	242	307	IPR003657	WRKY domain
SMART	SM00774	5.2E-37	247	306	IPR003657	WRKY domain
Pfam	PF03106	1.7E-24	248	305	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0070370	Biological Process	cellular heat acclimation
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 316 aa Download sequence Send to blast
MDNQRAIPKF KSSIPSPLSP SPYFFMPPGL TQADLLDSPL LFTSSNILPS PTTGTFPAQS 60 LNWNNNGLLE DRNEIKPEED GKEHFDFSFT NNHTSPLFLP PLVVKPLIQP DVPKHEPRSL 120 HTSNRTSEDG YNWRKYGQKQ VKGSENPRSY FKCTYPNCIT KKKVETSLVN GHITEIIYKG 180 SHNHPKPQSI GRAASTGATY NVDFHKSNSH GNGKEFGEDE ASGKRKREEN MKEPRVVVQT 240 TSDIDILEDG YRWRKYGQKV VKGNPNPRSY YKCTFTGCCV RKHVERAFQD AKSVITTYEG 300 KHNHQIPTPR KCHTLI

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	1e-34	128	308	8	78	Probable WRKY transcription factor 4
2lex_A	1e-34	128	308	8	78	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Functions with WRKY25 and WRKY33 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway (PubMed:21336597). {ECO:0000250\|UniProtKB:Q9SI37, ECO:0000269\|PubMed:21336597}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00494	DAP	Transfer from AT5G07100	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	KFK25104.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid (PubMed:11449049). Induced by heat stress (PubMed:21336597). {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:21336597}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF224699	9e-96	AF224699.1 Arabidopsis thaliana WRKY DNA-binding protein 26 (WRKY26) mRNA, complete cds.
GenBank	AK117545	9e-96	AK117545.1 Arabidopsis thaliana At5g07100 mRNA for SPF1 like protein, complete cds, clone: RAFL17-19-L10.
GenBank	BT026349	9e-96	BT026349.1 Arabidopsis thaliana At5g07100 gene, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_002871249.1	1e-165	probable WRKY transcription factor 26 isoform X1
Swissprot	Q9C5T3	1e-155	WRK26_ARATH; Probable WRKY transcription factor 26
TrEMBL	A0A087G5F2	0.0	A0A087G5F2_ARAAL; Uncharacterized protein
STRING	A0A087G5F2	0.0	(Arabis alpina)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM15291	15	18

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G07100.1	1e-142	WRKY DNA-binding protein 26

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444]