PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Jatropheae; Jatropha

Family

ARF

Protein Properties

Length: 1017aa MW: 112142 Da PI: 5.0774

Description

ARF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Jcr4S00712.20	genome	Kazusa	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	B3	54.4	2.3e-17	189	269	1	76
EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--T CS B3 1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLke 76 f+k+lt sd++++g +++p++ ae+ ++++ + ++l+++d++ + W++++iyr++++r++lt+GW+ Fv +++Lk+ Jcr4S00712.20 189 FCKTLTASDTSTHGGFSVPRRAAEKLfppldytMQPP-T-QELVVRDLHDNIWTFRHIYRGQPKRHLLTTGWSLFVGSKRLKA 269 99*******************999*954444.4.38*************************************96 PP
2	Auxin_resp	117.2	1.4e-38	345	428	1	83
Auxin_resp 1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 aahaa+++s+F+++YnPra++seFv+++ k++ka++ ++vsvGmRf m+fete+s +rr++Gt+vg+sdldp+rWp+SkWr+L+ Jcr4S00712.20 345 AAHAAANRSPFTIFYNPRACPSEFVIPLTKYRKAIYgTQVSVGMRFGMMFETEESGKRRYMGTIVGISDLDPLRWPGSKWRNLQ 428 79********************************9*******************************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF101936	1.24E-34	176	267	IPR015300	DNA-binding pseudobarrel domain
Gene3D	G3DSA:2.40.330.10	2.5E-29	182	269	IPR015300	DNA-binding pseudobarrel domain
CDD	cd10017	1.16E-16	188	269	No hit	No description
Pfam	PF02362	5.4E-15	189	269	IPR003340	B3 DNA binding domain
SMART	SM01019	1.6E-10	189	280	IPR003340	B3 DNA binding domain
Pfam	PF06507	1.4E-33	345	428	IPR010525	Auxin response factor
Pfam	PF02309	8.2E-10	894	990	IPR033389	AUX/IAA domain
PROSITE profile	PS51745	26.393	903	987	IPR000270	PB1 domain
SuperFamily	SSF54277	2.6E-8	916	982	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009734	Biological Process	auxin-activated signaling pathway
GO:0009908	Biological Process	flower development
GO:0009942	Biological Process	longitudinal axis specification
GO:0010305	Biological Process	leaf vascular tissue pattern formation
GO:0048364	Biological Process	root development
GO:0048507	Biological Process	meristem development
GO:0005634	Cellular Component	nucleus
GO:0016020	Cellular Component	membrane
GO:0042802	Molecular Function	identical protein binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 1017 aa Download sequence Send to blast
MMMGSVEEKI KAGGLVNGAQ TNLLEEMKLL KEIQDHSGTR KTINSELWYA CAGPLVSLPQ 60 VGSLVYYFPQ GHSEQVAVST KRSATSQIPN YPNLPSQLLC QVHNVTLHAD KDTDEIYAQM 120 SLQPVNSPLM SGQKLCRLIS FTIDVMNGKE WICNRSATRS KIEGPALEKD VFPIPDFGLK 180 PSKHPSEFFC KTLTASDTST HGGFSVPRRA AEKLFPPLDY TMQPPTQELV VRDLHDNIWT 240 FRHIYRGQPK RHLLTTGWSL FVGSKRLKAD GRDRNICNEL PPPPTIASGK SCVQSFLQND 300 KKLYCAMDEK SQLLVGVRRA NRQQTTLPSS VLSADSMHIG VLAAAAHAAA NRSPFTIFYN 360 PRACPSEFVI PLTKYRKAIY GTQVSVGMRF GMMFETEESG KRRYMGTIVG ISDLDPLRWP 420 GSKWRNLQVE WDESGCSDKQ NRVSSWEIET PESLFIFPSL TSGLKRPLHS GMLGGETEWS 480 NLIKRPLIWL PEHGNGNFPY SSIPNLYSER LYKMLMKPQV NYPGICESAL QELCAAKATP 540 VDDVKTMQGP INQLNQSVGM SVENQNYSQF CANQSDVINP SASKINTPGN LHPPCTVENQ 600 TPDGINVEKL KSEPEHSADQ ISQVTSTGEC NEEKTSSSPT NLHNCSNQLE FQNQNQAHLH 660 AQTNVWPMQQ LLEPSTLHSQ QINIPQADSN VLNGSLPFLD TDEWISNPSS CIPLPGIYGS 720 SGPLSMFGLQ EQSSILPEAI NPSLPLMNQD LWDQQLNNLR FLSPPSQLVP LAQQDLCSLN 780 SSGAKDLSDE SNDQSGIYGS LSVDVGNGGS AVIDPSVSNT LLDELCTSKD ADFQNPSDCL 840 VGNLSSSQDV QSQITSASLA DSQAFSQQDF PDSSGGTSSS NVDIDKGNYM QNNSWQQVAP 900 RVRTYTKVQK AGSVGRSIDV SSFRNYEELC SAIECMFGLE GLLNNPRESG WKLVYVDYEN 960 DVLLIGDDPW EEFVGCVRCI RILSPSEVQQ MSEEGMKLLN NANINGLNTS VTEGSHA

Sequence ? help Back to Top

Protein Sequence Length: 1017 aa Download sequence Send to blast

MMMGSVEEKI KAGGLVNGAQ TNLLEEMKLL KEIQDHSGTR KTINSELWYA CAGPLVSLPQ  60
VGSLVYYFPQ GHSEQVAVST KRSATSQIPN YPNLPSQLLC QVHNVTLHAD KDTDEIYAQM  120
SLQPVNSPLM SGQKLCRLIS FTIDVMNGKE WICNRSATRS KIEGPALEKD VFPIPDFGLK  180
PSKHPSEFFC KTLTASDTST HGGFSVPRRA AEKLFPPLDY TMQPPTQELV VRDLHDNIWT  240
FRHIYRGQPK RHLLTTGWSL FVGSKRLKAD GRDRNICNEL PPPPTIASGK SCVQSFLQND  300
KKLYCAMDEK SQLLVGVRRA NRQQTTLPSS VLSADSMHIG VLAAAAHAAA NRSPFTIFYN  360
PRACPSEFVI PLTKYRKAIY GTQVSVGMRF GMMFETEESG KRRYMGTIVG ISDLDPLRWP  420
GSKWRNLQVE WDESGCSDKQ NRVSSWEIET PESLFIFPSL TSGLKRPLHS GMLGGETEWS  480
NLIKRPLIWL PEHGNGNFPY SSIPNLYSER LYKMLMKPQV NYPGICESAL QELCAAKATP  540
VDDVKTMQGP INQLNQSVGM SVENQNYSQF CANQSDVINP SASKINTPGN LHPPCTVENQ  600
TPDGINVEKL KSEPEHSADQ ISQVTSTGEC NEEKTSSSPT NLHNCSNQLE FQNQNQAHLH  660
AQTNVWPMQQ LLEPSTLHSQ QINIPQADSN VLNGSLPFLD TDEWISNPSS CIPLPGIYGS  720
SGPLSMFGLQ EQSSILPEAI NPSLPLMNQD LWDQQLNNLR FLSPPSQLVP LAQQDLCSLN  780
SSGAKDLSDE SNDQSGIYGS LSVDVGNGGS AVIDPSVSNT LLDELCTSKD ADFQNPSDCL  840
VGNLSSSQDV QSQITSASLA DSQAFSQQDF PDSSGGTSSS NVDIDKGNYM QNNSWQQVAP  900
RVRTYTKVQK AGSVGRSIDV SSFRNYEELC SAIECMFGLE GLLNNPRESG WKLVYVDYEN  960
DVLLIGDDPW EEFVGCVRCI RILSPSEVQQ MSEEGMKLLN NANINGLNTS VTEGSHA

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4ldu_A	0.0	2	453	1	392	Auxin response factor 5
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7. May be necessary to counteract AMP1 activity. {ECO:0000269\|PubMed:12036261, ECO:0000269\|PubMed:14973283, ECO:0000269\|PubMed:17553903}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00153	DAP	Transfer from AT1G19850	Download

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_012073833.1	0.0	auxin response factor 5 isoform X1
Swissprot	P93024	0.0	ARFE_ARATH; Auxin response factor 5
TrEMBL	A0A067KLD5	0.0	A0A067KLD5_JATCU; Auxin response factor
STRING	cassava4.1_001105m	0.0	(Manihot esculenta)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF8991	32	41

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G19850.1	0.0	ARF family protein

Publications ? help Back to Top
Chen MK,Wilson RL,Palme K,Ditengou FA,Shpak ED ERECTA family genes regulate auxin transport in the shoot apical meristem and forming leaf primordia. Plant Physiol., 2013. 162(4): p. 1978-91 [PMID:23821653] Huang JB, et al. ROP3 GTPase contributes to polar auxin transport and auxin responses and is important for embryogenesis and seedling growth in Arabidopsis. Plant Cell, 2014. 26(9): p. 3501-18 [PMID:25217509] Qi J, et al. Auxin depletion from leaf primordia contributes to organ patterning. Proc. Natl. Acad. Sci. U.S.A., 2014. 111(52): p. 18769-74 [PMID:25512543] Crawford BC, et al. Plant development. Genetic control of distal stem cell fate within root and embryonic meristems. Science, 2015. 347(6222): p. 655-9 [PMID:25612610] Zhang Y, et al. Regulation of oncogene expression in T-DNA-transformed host plant cells. PLoS Pathog., 2015. 11(1): p. e1004620 [PMID:25615824] Robert HS, et al. Plant embryogenesis requires AUX/LAX-mediated auxin influx. Development, 2015. 142(4): p. 702-11 [PMID:25617434] Krogan NT,Berleth T The identification and characterization of specific ARF-Aux/IAA regulatory modules in plant growth and development. Plant Signal Behav, 2015. 10(4): p. e992748 [PMID:25830553] Ckurshumova W,Berleth T Overcoming recalcitrance - Auxin response factor functions in plant regeneration. Plant Signal Behav, 2015. 10(7): p. e993293 [PMID:26098229] Wendrich JR, et al. A set of domain-specific markers in the Arabidopsis embryo. Plant Reprod, 2015. 28(3-4): p. 153-60 [PMID:26216537] Wu MF, et al. Auxin-regulated chromatin switch directs acquisition of flower primordium founder fate. Elife, 2015. 4: p. e09269 [PMID:26460543] Yamaguchi N,Jeong CW,Nole-Wilson S,Krizek BA,Wagner D AINTEGUMENTA and AINTEGUMENTA-LIKE6/PLETHORA3 Induce LEAFY Expression in Response to Auxin to Promote the Onset of Flower Formation in Arabidopsis. Plant Physiol., 2016. 170(1): p. 283-93 [PMID:26537561] Müller CJ, et al. PHABULOSA Mediates an Auxin Signaling Loop to Regulate Vascular Patterning in Arabidopsis. Plant Physiol., 2016. 170(2): p. 956-70 [PMID:26637548] Herud O,Weijers D,Lau S,Jürgens G Auxin responsiveness of the MONOPTEROS-BODENLOS module in primary root initiation critically depends on the nuclear import kinetics of the Aux/IAA inhibitor BODENLOS. Plant J., 2016. 85(2): p. 269-77 [PMID:26714008] Luo S, et al. Constitutive Expression of OsIAA9 Affects Starch Granules Accumulation and Root Gravitropic Response in Arabidopsis. Front Plant Sci, 2015. 6: p. 1156 [PMID:26734051] Yamaguchi N,Wu MF,Winter CM,Wagner D LEAFY and Polar Auxin Transport Coordinately Regulate Arabidopsis Flower Development. Plants (Basel), 2014. 3(2): p. 251-65 [PMID:27135503] Krogan NT,Marcos D,Weiner AI,Berleth T The auxin response factor MONOPTEROS controls meristem function and organogenesis in both the shoot and root through the direct regulation of PIN genes. New Phytol., 2016. 212(1): p. 42-50 [PMID:27441727] Fendrych M,Leung J,Friml J TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. Elife, 2018. [PMID:27627746] Matthes M,Torres-Ruiz RA Boronic acid treatment phenocopies monopteros by affecting PIN1 membrane stability and polar auxin transport in Arabidopsis thaliana embryos. Development, 2016. 143(21): p. 4053-4062 [PMID:27697905] Bhatia N, et al. Auxin Acts through MONOPTEROS to Regulate Plant Cell Polarity and Pattern Phyllotaxis. Curr. Biol., 2016. 26(23): p. 3202-3208 [PMID:27818174] Wójcikowska B,Gaj MD Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis. Plant Cell Rep., 2017. 36(6): p. 843-858 [PMID:28255787] Möller BK, et al. Auxin response cell-autonomously controls ground tissue initiation in the early Arabidopsis embryo. Proc. Natl. Acad. Sci. U.S.A., 2017. 114(12): p. E2533-E2539 [PMID:28265057] Carey NS,Krogan NT The role of AUXIN RESPONSE FACTORs in the development and differential growth of inflorescence stems. Plant Signal Behav, 2017. 12(4): p. e1307492 [PMID:28340328] Guan C, et al. Spatial Auxin Signaling Controls Leaf Flattening in Arabidopsis. Curr. Biol., 2017. 27(19): p. 2940-2950.e4 [PMID:28943086] Gaillochet C, et al. Control of plant cell fate transitions by transcriptional and hormonal signals. Elife, 2018. [PMID:29058667] Zheng K, et al. Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis. Front Plant Sci, 2017. 8: p. 1813 [PMID:29114256] Liu Z, et al. ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis. Plant Cell Physiol., 2018. 59(1): p. 179-189 [PMID:29145642]