PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Glyma.20G107500.1.p

Common Name

GLYMA_20G107500

Organism

Glycine max

Taxonomic ID

3847

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Glycine; Soja

Family

bHLH

Protein Properties

Length: 578aa MW: 65587.9 Da PI: 4.9293

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Glyma.20G107500.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	40	7.2e-13	345	388	7	55
HHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS HLH 7 erErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 +Er+RR+++N+++ +Lr+l+P + Kl+ a+iL A+eY+k+Lq Glyma.20G107500.1.p 345 VAERKRRKKLNDRLYNLRSLVPRiS------KLDRASILGDAIEYVKDLQ 388 68*******************66......****************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	1.4E-19	57	203	IPR025610	Transcription factor MYC/MYB N-terminal
CDD	cd00083	2.59E-13	337	392	No hit	No description
PROSITE profile	PS50888	15.866	338	387	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.31E-17	341	401	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	3.7E-17	344	401	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	4.8E-16	344	393	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	2.1E-10	345	388	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd04873	0.00186	472	536	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 578 aa Download sequence Send to blast
MDISFCFFCF FWSVFSSILL LLLVKGSNHS HFAFVSSKRK FHYHHPGDMN IIMQNLVERL 60 RPLVGLNGWD YCIYWKLSED QRFLEWLGCC CAGTESNQNA GEEHIFPVSS VASCRDSTYP 120 HPRTKPCDLL SQLSTSIPID NSGIHAQTLL TNQPNWVNYS NGMDPNILEE TIGTQVLISV 180 PGGLVELFVT KQVPEDHQLI DYVINQCIEA VNHSMSFHID ENSMSNMQSN PLIGDENEGN 240 NNSRDTSTLQ NMSSQWTSAV LQTNQEDQEH EHEHDTYQKS LMTTTDSQYV EPLEAKEKQE 300 EDKDLLKNVV GRSDSMSDCS DQNEEEEDGK YRRRNGKGNQ SKNLVAERKR RKKLNDRLYN 360 LRSLVPRISK LDRASILGDA IEYVKDLQKQ VKELQDELEE NADTESNCMN CVSELGPNAE 420 HDKAQTGLHV GTSGNGYVSK QKQEGTTVID KQTQQMEPQV EVALIDGNEY FVKVFCEHRP 480 DGFVKLMEAL NTIGMDVVHA TVTSHTGLVS NVFKVEKKDS ETVEAEDVRD SLLELMRNRY 540 RGWTHEMTAT SGNSVESDQH QLHNHNQMGA YPHEFHS*

Sequence ? help Back to Top

Protein Sequence Length: 578 aa Download sequence Send to blast

MDISFCFFCF FWSVFSSILL LLLVKGSNHS HFAFVSSKRK FHYHHPGDMN IIMQNLVERL  60
RPLVGLNGWD YCIYWKLSED QRFLEWLGCC CAGTESNQNA GEEHIFPVSS VASCRDSTYP  120
HPRTKPCDLL SQLSTSIPID NSGIHAQTLL TNQPNWVNYS NGMDPNILEE TIGTQVLISV  180
PGGLVELFVT KQVPEDHQLI DYVINQCIEA VNHSMSFHID ENSMSNMQSN PLIGDENEGN  240
NNSRDTSTLQ NMSSQWTSAV LQTNQEDQEH EHEHDTYQKS LMTTTDSQYV EPLEAKEKQE  300
EDKDLLKNVV GRSDSMSDCS DQNEEEEDGK YRRRNGKGNQ SKNLVAERKR RKKLNDRLYN  360
LRSLVPRISK LDRASILGDA IEYVKDLQKQ VKELQDELEE NADTESNCMN CVSELGPNAE  420
HDKAQTGLHV GTSGNGYVSK QKQEGTTVID KQTQQMEPQV EVALIDGNEY FVKVFCEHRP  480
DGFVKLMEAL NTIGMDVVHA TVTSHTGLVS NVFKVEKKDS ETVEAEDVRD SLLELMRNRY  540
RGWTHEMTAT SGNSVESDQH QLHNHNQMGA YPHEFHS*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	331	352	RRRNGKGNQSKNLVAERKRRKK
2	346	353	ERKRRKKL

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: First observed a bud stage of 0.6-mm, shortly after microspore release from the postmeiotic tetrads. Later restricted within the tapetum, microspores and anther locule. Still visible within the haploid nuclei, each of which had migrated to the pollen cell wall prior to pollen mitosis I. Not expressed later. {ECO:0000269\|PubMed:12535353}.
Uniprot	TISSUE SPECIFICITY: Mostly expressed in closed, post-meiotic buds, and, to a lower extent, in pre-meiotic buds. Detected in leaves, stems, and flowers. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:24804694}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Glyma.20G107500.1.p

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AP015041	1e-131	AP015041.1 Vigna angularis var. angularis DNA, chromosome 8, almost complete sequence, cultivar: Shumari.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_025983204.1	0.0	transcription factor ABORTED MICROSPORES
Swissprot	Q9ZVX2	1e-138	AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBL	A0A0R0ELN4	0.0	A0A0R0ELN4_SOYBN; Uncharacterized protein
TrEMBL	A0A445F3I6	0.0	A0A445F3I6_GLYSO; Transcription factor ABORTED MICROSPORES
STRING	GLYMA20G24170.2	0.0	(Glycine max)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF4225	32	47
Representative plant	OGRP3704	13	23

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G16910.1	1e-138	bHLH family protein

Link Out ? help Back to Top
Phytozome	Glyma.20G107500.1.p

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Jiang J,Zhang Z,Cao J Pollen wall development: the associated enzymes and metabolic pathways. Plant Biol (Stuttg), 2013. 15(2): p. 249-63 [PMID:23252839] Xu J, et al. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis. Plant Cell, 2014. 26(4): p. 1544-1556 [PMID:24781116] Qian H, et al. Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis. Ecotoxicology, 2015. 24(1): p. 163-71 [PMID:25348600] Xiong SX, et al. The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana. Plant J., 2016. 88(6): p. 936-946 [PMID:27460657] Ferguson AC, et al. Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis. New Phytol., 2017. 213(2): p. 778-790 [PMID:27787905] Lou Y, et al. Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana. New Phytol., 2018. 217(1): p. 378-391 [PMID:28940573] Bi H,Fan W,Zhang P C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis. Front Plant Sci, 2017. 8: p. 1689 [PMID:29021807]