PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Glycine; Soja

Family

ERF

Protein Properties

Length: 276aa MW: 30621.8 Da PI: 5.4364

Description

ERF family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Glyma.19G113100.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	58	2.3e-18	36	85	2	55
AP2 2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 ++ GVr+++ +grWvAeI+d + ++ r +lg+f taeeAa+a+++a+ l+g Glyma.19G113100.1.p 36 KFVGVRQRP-SGRWVAEIKDTT---QKIRMWLGTFETAEEAARAYDEAACLLRG 85 799****.7*****32...35********************99987 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:3.30.730.10	6.9E-28	36	93	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	19.585	36	93	IPR001471	AP2/ERF domain
Pfam	PF00847	5.4E-13	36	85	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	3.27E-19	36	93	IPR016177	DNA-binding domain
SMART	SM00380	1.6E-35	36	99	IPR001471	AP2/ERF domain
CDD	cd00018	3.48E-32	36	92	No hit	No description
PRINTS	PR00367	9.3E-8	37	48	IPR001471	AP2/ERF domain
PRINTS	PR00367	9.3E-8	59	75	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0000302	Biological Process	response to reactive oxygen species
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009723	Biological Process	response to ethylene
GO:0035865	Biological Process	cellular response to potassium ion
GO:0048528	Biological Process	post-embryonic root development
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 276 aa Download sequence Send to blast
MEIQFQQPNL QHQKSGIAIT KGGKFKGRSR NNNTNKFVGV RQRPSGRWVA EIKDTTQKIR 60 MWLGTFETAE EAARAYDEAA CLLRGSNTRT NFITHVSLDS PLASRIRNLL NNRKGIKKEE 120 DANANANNAP APRVSTTSSA TSTGTSSNSS NGNDNNDQSL STVTTQSSNL FDDAYKPDLS 180 NCREEFESGH QSNTSWCFGP VFDRFPMAQI FDIPKIDDGL TNAADLDLSE FERMKVERQI 240 SASLYAINGV QEYMETVQDS NETLWDLPPL CSLFC*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5wx9_A	8e-15	27	102	5	81	Ethylene-responsive transcription factor ERF096
Search in ModeBase

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in roots, root hairs and leaves (PubMed:17827349). Expressed in root epidermis and root hairs (PubMed:23077241). {ECO:0000269\|PubMed:17827349, ECO:0000269\|PubMed:23077241}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in symbiotic nodule signaling in response to rhizobial Nod factors (NFs) (PubMed:17449807, PubMed:17827349). Binds to the GCC-box (NF-responsive box) of ENOD11 promoter. Acts as transcriptional activator of NF-responsive box-containing target gene promoters in root hairs (PubMed:17827349). Functions as a transcriptional regulator required for root infection by symbiotic rhizobia, infection thread (IT) formation and maintenance, and nodule development. Necessary for NF-induced gene expression and spontaneous nodulation activated by CCAMK. Functions downstream of CCAMK to activate nodulation gene expression (PubMed:17449807). Involved in early stages of root nodule development. Functions redundantly with ERN2. Is essential with ERN2 for the initiation of root hair infection, and nodule organogenesis and development. Required for accurate expression of the NF signaling genes ENOD11 and ENOD12 (PubMed:23077241, PubMed:27208242). {ECO:0000269\|PubMed:17449807, ECO:0000269\|PubMed:17827349, ECO:0000269\|PubMed:23077241, ECO:0000269\|PubMed:27208242}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00521	DAP	Transfer from AT5G19790	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Glyma.19G113100.1.p

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced in roots from 1 to 21 day after inoculation with Sinorhizobium meliloti (PubMed:17449807). Induced by Nod factors in root hairs (PubMed:17827349). {ECO:0000269\|PubMed:17449807, ECO:0000269\|PubMed:17827349}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AP015037	1e-155	AP015037.1 Vigna angularis var. angularis DNA, chromosome 4, almost complete sequence, cultivar: Shumari.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006604239.1	0.0	ethylene-responsive transcription factor ERN1
Refseq	XP_028217785.1	0.0	ethylene-responsive transcription factor ERN1-like
Swissprot	A2Q5W1	1e-143	ERN1_MEDTR; Ethylene-responsive transcription factor ERN1
TrEMBL	A0A445FF21	0.0	A0A445FF21_GLYSO; Ethylene-responsive transcription factor ERN1
TrEMBL	I1N899	0.0	I1N899_SOYBN; Uncharacterized protein
STRING	GLYMA19G29000.1	0.0	(Glycine max)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF3341	30	69
Representative plant	OGRP6	16	1718

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G19790.1	1e-29	related to AP2 11

Link Out ? help Back to Top
Phytozome	Glyma.19G113100.1.p
Entrez Gene	100796898

Publications ? help Back to Top
Cerri MR, et al. Medicago truncatula ERN transcription factors: regulatory interplay with NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial infection. Plant Physiol., 2012. 160(4): p. 2155-72 [PMID:23077241] Tang H, et al. An improved genome release (version Mt4.0) for the model legume Medicago truncatula. BMC Genomics, 2014. 15: p. 312 [PMID:24767513] Cerri MR, et al. The Symbiosis-Related ERN Transcription Factors Act in Concert to Coordinate Rhizobial Host Root Infection. Plant Physiol., 2016. 171(2): p. 1037-54 [PMID:27208242]