PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Glyma.12G042800.2.p

Common Name

GLYMA_12G042800, LOC100815751

Organism

Glycine max

Taxonomic ID

3847

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Glycine; Soja

Family

bHLH

Protein Properties

Length: 403aa MW: 44211.7 Da PI: 7.6901

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Glyma.12G042800.2.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	28.7	2.3e-09	202	249	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +h+ +Er RR++i +++ L++l+P + +k Ka +L + ++Y++sLq Glyma.12G042800.2.p 202 SHSLAERVRREKISERMKYLQDLIPGC----NKVAGKAGMLDEIINYVQSLQ 249 8***********************9....677***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	1.06E-16	196	267	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	7.73E-10	196	253	No hit	No description
PROSITE profile	PS50888	15.275	198	248	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	1.8E-17	198	265	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.6E-6	202	249	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	8.3E-10	204	254	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 403 aa Download sequence Send to blast
MLHCLNTSCS DITVLERQRE ATIKCQNHQP PYLTDFNAVF PSSSSSSSSS SSSSHSQGLL 60 MMCSDSSLGQ VLTHSVKPDP GVWPEFGFLP AISRTCSRDG DLVSPKENMA SGKENAKKRK 120 PQNSKVVAEI DNNKDKDKRV KVTGEEGESK VTEHHTRNKN AKSNANKNNR ETSADTSKGS 180 EVQNQKPDYI HVRARRGQAT DSHSLAERVR REKISERMKY LQDLIPGCNK VAGKAGMLDE 240 IINYVQSLQR QVEFLSMKLA AVNPRLDFNI DELFAKEVFP SCAQSFPNIG IPSDMSISNN 300 PSYLQFNSAQ QLVSCCGGLI NSMGISPPNM GLRTNIISTS TVPLPETFLD SSCFAQILPS 360 SNWEGGDFQS LYNVAFDQGR TASFPPQPFT GLVEASNLKM EM*

Sequence ? help Back to Top

Protein Sequence Length: 403 aa Download sequence Send to blast

MLHCLNTSCS DITVLERQRE ATIKCQNHQP PYLTDFNAVF PSSSSSSSSS SSSSHSQGLL  60
MMCSDSSLGQ VLTHSVKPDP GVWPEFGFLP AISRTCSRDG DLVSPKENMA SGKENAKKRK  120
PQNSKVVAEI DNNKDKDKRV KVTGEEGESK VTEHHTRNKN AKSNANKNNR ETSADTSKGS  180
EVQNQKPDYI HVRARRGQAT DSHSLAERVR REKISERMKY LQDLIPGCNK VAGKAGMLDE  240
IINYVQSLQR QVEFLSMKLA AVNPRLDFNI DELFAKEVFP SCAQSFPNIG IPSDMSISNN  300
PSYLQFNSAQ QLVSCCGGLI NSMGISPPNM GLRTNIISTS TVPLPETFLD SSCFAQILPS  360
SNWEGGDFQS LYNVAFDQGR TASFPPQPFT GLVEASNLKM EM*

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Gma.9167	0.0	cotyledon\| flower\| hypocotyl\| leaf\| meristem

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and stems. {ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light. {ECO:0000269\|PubMed:18988809, ECO:0000269\|PubMed:24130508}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Glyma.12G042800.2.p

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level). {ECO:0000269\|PubMed:24101505}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	BT098303	0.0	BT098303.1 Soybean clone JCVI-FLGm-23G1 unknown mRNA.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_003541034.1	0.0	transcription factor bHLH63 isoform X2
Swissprot	Q8GY61	5e-55	BH063_ARATH; Transcription factor bHLH63
TrEMBL	I1LQ31	0.0	I1LQ31_SOYBN; Uncharacterized protein
STRING	GLYMA12G04670.1	0.0	(Glycine max)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G34530.1	3e-55	cryptochrome-interacting basic-helix-loop-helix 1

Link Out ? help Back to Top
Phytozome	Glyma.12G042800.2.p
Entrez Gene	100815751

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Yang D,Zhao W,Meng Y,Li H,Liu B A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Sci China Life Sci, 2015. 58(3): p. 261-9 [PMID:25651969] Katsura Y, et al. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015. 5: p. 14589 [PMID:26423353] Taslimi A, et al. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol., 2016. 12(6): p. 425-30 [PMID:27065233] Liu Q, et al. The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases. Plant Cell Physiol., 2016. 57(10): p. 2175-2186 [PMID:27516416] Pathak GP, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res., 2017. 45(20): p. e167 [PMID:28431041] Duan L, et al. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017. 8(1): p. 547 [PMID:28916751] Quejada JR, et al. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res., 2017. 45(20): p. e172 [PMID:29040770] Liu Y, et al. CIB1 and CO interact to mediate CRY2-dependent regulation of flowering. EMBO Rep., 2019. [PMID:30126927]