PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GSVIVT01024008001

Common Name

VIT_03s0038g02540

Organism

Vitis vinifera

Taxonomic ID

29760

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; rosids incertae sedis; Vitales; Vitaceae; Vitis

Family

bHLH

Protein Properties

Length: 574aa MW: 64156.6 Da PI: 5.1867

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GSVIVT01024008001	genome	Genoscope	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	39.1	1.3e-12	333	377	6	55
HHHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS HLH 6 nerErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 +ErrRR+++N+++ Lr+l+Pk + Kl+ a+iL A+e++k+Lq GSVIVT01024008001 333 IDAERRRRKKLNDRLYALRSLVPKiS------KLDRASILGDAIEFVKELQ 377 579*******************66......****************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	3.0E-22	43	194	IPR025610	Transcription factor MYC/MYB N-terminal
PROSITE profile	PS50888	16.05	327	376	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	2.75E-16	329	390	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	3.39E-13	329	379	No hit	No description
Pfam	PF00010	2.3E-10	332	377	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	2.2E-15	333	382	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	3.5E-16	334	390	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0048657	Biological Process	anther wall tapetum cell differentiation
GO:0003677	Molecular Function	DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0009029	anatomy	stamen
PO:0009032	anatomy	petal
PO:0007016	developmental stage	whole plant flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 574 aa Download sequence Send to blast
MFQNQVNRTY FDNREHSSPV TSRFFEVGMM LIAGETINMV QSLMERLRPL VGLKSRDWDY 60 CVLWKLSEDQ RFLDWMDCCC AGGGENSTQN GGEEHLFPVS SVLPCRDAMS QHPRTKSCDL 120 LAQLPSSISL DSGIHGQTLI SNQPRWLNFC NSSDSSILEE TVGTGLLIPV LGGLIELFVA 180 KQVAEDQHVI NFVTTQCHMI SMEQEAMMNS SNINSIFSVN VNGGNADENQ KDPNNHFQAP 240 ISPVTAMEDL NDLPISVDQI RLCSSPMNFL QQFSYTSESS IKNDQLGDDH KDLSAKRTAN 300 QADSVSDCSD QIDDDDDLKF QRRTGKGAQS KNIDAERRRR KKLNDRLYAL RSLVPKISKL 360 DRASILGDAI EFVKELQKQA KDLQDELEEN SEDEVNIGPK TENEETQNRF LMGAAGNGIA 420 ASACRPPSAK QNHETDQITD DKAQQMEPQV EVAQIEGNDF FVKVFCEHKA GGFVRLMEAL 480 SSLGLEVTNA NVTSCKGLVS NLFKVEKRDS EMVQADHVRD SLLELTKNPS EKWHGQMAYA 540 SENGGGLDFH NHHHHHHLHS HLLSSHHLHH LQN*

Sequence ? help Back to Top

Protein Sequence Length: 574 aa Download sequence Send to blast

MFQNQVNRTY FDNREHSSPV TSRFFEVGMM LIAGETINMV QSLMERLRPL VGLKSRDWDY  60
CVLWKLSEDQ RFLDWMDCCC AGGGENSTQN GGEEHLFPVS SVLPCRDAMS QHPRTKSCDL  120
LAQLPSSISL DSGIHGQTLI SNQPRWLNFC NSSDSSILEE TVGTGLLIPV LGGLIELFVA  180
KQVAEDQHVI NFVTTQCHMI SMEQEAMMNS SNINSIFSVN VNGGNADENQ KDPNNHFQAP  240
ISPVTAMEDL NDLPISVDQI RLCSSPMNFL QQFSYTSESS IKNDQLGDDH KDLSAKRTAN  300
QADSVSDCSD QIDDDDDLKF QRRTGKGAQS KNIDAERRRR KKLNDRLYAL RSLVPKISKL  360
DRASILGDAI EFVKELQKQA KDLQDELEEN SEDEVNIGPK TENEETQNRF LMGAAGNGIA  420
ASACRPPSAK QNHETDQITD DKAQQMEPQV EVAQIEGNDF FVKVFCEHKA GGFVRLMEAL  480
SSLGLEVTNA NVTSCKGLVS NLFKVEKRDS EMVQADHVRD SLLELTKNPS EKWHGQMAYA  540
SENGGGLDFH NHHHHHHLHS HLLSSHHLHH LQN*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	335	342	ERRRRKKL

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: First observed a bud stage of 0.6-mm, shortly after microspore release from the postmeiotic tetrads. Later restricted within the tapetum, microspores and anther locule. Still visible within the haploid nuclei, each of which had migrated to the pollen cell wall prior to pollen mitosis I. Not expressed later. {ECO:0000269\|PubMed:12535353}.
Uniprot	TISSUE SPECIFICITY: Mostly expressed in closed, post-meiotic buds, and, to a lower extent, in pre-meiotic buds. Detected in leaves, stems, and flowers. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:24804694}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KR919615	0.0	KR919615.1 Vitis vinifera aborted microspores-like protein mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_019074042.1	0.0	PREDICTED: transcription factor ABORTED MICROSPORES isoform X4
Swissprot	Q9ZVX2	1e-149	AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBL	F6I0Z6	0.0	F6I0Z6_VITVI; Uncharacterized protein
STRING	VIT_03s0038g02540.t01	0.0	(Vitis vinifera)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Representative plant	OGRP3704	13	23

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G16910.1	5e-76	bHLH family protein

Link Out ? help Back to Top
Phytozome	GSVIVT01024008001

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Jiang J,Zhang Z,Cao J Pollen wall development: the associated enzymes and metabolic pathways. Plant Biol (Stuttg), 2013. 15(2): p. 249-63 [PMID:23252839] Xu J, et al. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis. Plant Cell, 2014. 26(4): p. 1544-1556 [PMID:24781116] Qian H, et al. Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis. Ecotoxicology, 2015. 24(1): p. 163-71 [PMID:25348600] Xiong SX, et al. The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana. Plant J., 2016. 88(6): p. 936-946 [PMID:27460657] Ferguson AC, et al. Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis. New Phytol., 2017. 213(2): p. 778-790 [PMID:27787905] Lou Y, et al. Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana. New Phytol., 2018. 217(1): p. 378-391 [PMID:28940573] Bi H,Fan W,Zhang P C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis. Front Plant Sci, 2017. 8: p. 1689 [PMID:29021807]