PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Cucsa.321840.1

Common Name

Csa_3G011620, LOC101205372

Organism

Cucumis sativus

Taxonomic ID

3659

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis

Family

bHLH

Protein Properties

Length: 471aa MW: 51181.3 Da PI: 7.7635

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Cucsa.321840.1	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	39.9	7.4e-13	286	331	4	54
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 +h e+Er+RR+++N++f Lr ++P+ K++Ka+ L Av YI++L Cucsa.321840.1 286 NHVEAERQRREKLNQKFYALRAVVPNV-----SKMDKASLLGDAVSYINEL 331 799*********************6.....5*************998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	2.2E-22	58	211	IPR025610	Transcription factor MYC/MYB N-terminal
PROSITE profile	PS50888	17.161	282	331	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.17E-14	285	336	No hit	No description
SuperFamily	SSF47459	4.71E-18	285	351	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	2.1E-10	286	331	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	1.6E-17	286	352	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	9.5E-17	288	337	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 471 aa Download sequence Send to blast
NASVMDAFMN SDLSSYWAPS AASSHSLHHP PPPQSSASTS TPPPDAPKSL PVFNQETLQQ 60 RLQALIDGAR ESWTYAIFWQ SSYDYSGGSV LGWGDGYYKG EEDKGKGKAK MVSSAAEQAH 120 RKKVLRELNS LISGSAAGPD DAVDEESQSF LNFSDYGFES NPTKNTTATA TATTSTTPSF 180 KPESGGMLNF GNGSLFSGHS QYVTNEQNEK KRSPASRSSN DEGILSFTSG VILPSSGKVK 240 SGDSDHSDLE ASAIREVDSC TKSLEPEKRP RKRGRKPANG REEPLNHVEA ERQRREKLNQ 300 KFYALRAVVP NVSKMDKASL LGDAVSYINE LKSKLQMAES EKTDMGKHLE LLKKEMGGKD 360 LGCYSNPNDE DLKTGKRKVM DMEIEVKIMG WDAMIRIQSN KKNHPAARLM TAFKDLDLEM 420 LHASVSVVND LMIQQATVKM GSRFYTQEQL KMALVARVGG GGSGGGGGIM *

Sequence ? help Back to Top

Protein Sequence Length: 471 aa Download sequence Send to blast

NASVMDAFMN SDLSSYWAPS AASSHSLHHP PPPQSSASTS TPPPDAPKSL PVFNQETLQQ  60
RLQALIDGAR ESWTYAIFWQ SSYDYSGGSV LGWGDGYYKG EEDKGKGKAK MVSSAAEQAH  120
RKKVLRELNS LISGSAAGPD DAVDEESQSF LNFSDYGFES NPTKNTTATA TATTSTTPSF  180
KPESGGMLNF GNGSLFSGHS QYVTNEQNEK KRSPASRSSN DEGILSFTSG VILPSSGKVK  240
SGDSDHSDLE ASAIREVDSC TKSLEPEKRP RKRGRKPANG REEPLNHVEA ERQRREKLNQ  300
KFYALRAVVP NVSKMDKASL LGDAVSYINE LKSKLQMAES EKTDMGKHLE LLKKEMGGKD  360
LGCYSNPNDE DLKTGKRKVM DMEIEVKIMG WDAMIRIQSN KKNHPAARLM TAFKDLDLEM  420
LHASVSVVND LMIQQATVKM GSRFYTQEQL KMALVARVGG GGSGGGGGIM *

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5gnj_A	1e-34	280	359	2	81	Transcription factor MYC2
5gnj_B	1e-34	280	359	2	81	Transcription factor MYC2
5gnj_E	1e-34	280	359	2	81	Transcription factor MYC2
5gnj_F	1e-34	280	359	2	81	Transcription factor MYC2
5gnj_G	1e-34	280	359	2	81	Transcription factor MYC2
5gnj_I	1e-34	280	359	2	81	Transcription factor MYC2
5gnj_M	1e-34	280	359	2	81	Transcription factor MYC2
5gnj_N	1e-34	280	359	2	81	Transcription factor MYC2
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	267	275	KRPRKRGRK

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator that binds to the G-box motif (5'-AACGTG-3') found in the promoter of the jasmonate-induced gene LAPA1 (PubMed:15231736). Acts as negative regulator of blue light-mediated photomorphogenesis and positively regulates root growth (PubMed:24483714). Promotes growth in response to the phytohormones abscisic acid (ABA) and jasmonate (JA) (PubMed:24483714). Binds to the G-box motif (5'-CACGTG-3') of the RBCS-3A gene promoter (PubMed:24483714). Acts downstream of the jasmonate (JA) receptor to orchestrate JA-mediated activation of plant responses (PubMed:28733419). Positively regulates both wound-responsive and pathogen-responsive genes through MYC2-targeted transcription factors (MTFs) involved in early response to JA (PubMed:28733419). With JA2L forms a transcription module that regulates wounding-responsive genes (PubMed:28733419). With ERF.C3 forms a transcription module that regulates pathogen-responsive genes (PubMed:28733419). Plays a critical role in orchestrating JA-mediated defense gene expression during Botrytis cinerea infection (PubMed:28733419). Regulates negatively defense responses to root-knot nematodes, potentially by mediating crosstalk among the hormones strigolactones, abscisic acid (ABA) and jasmonate (JA) (PubMed:30576511). Regulates the termination of JA-mediated defense responses by specifically binding the G-box (5'-CACATG-3') motifs in the promoters of MTB1, MTB2 and MTB3, which are transcription factors that negatively regulates JA signaling (PubMed:30610166). May be involved in JA-induced chilling tolerance, possibly by ameliorating the antioxidant enzyme system of fruit and increasing proline and lycopene levels (PubMed:29528226). {ECO:0000269\|PubMed:15231736, ECO:0000269\|PubMed:24483714, ECO:0000269\|PubMed:28733419, ECO:0000269\|PubMed:29528226, ECO:0000269\|PubMed:30576511, ECO:0000269\|PubMed:30610166}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by methyl jasmonate (PubMed:15231736, PubMed:28733419). Induced by wounding (PubMed:15231736). Induced by infection with the fungal pathogen Botrytis cinerea (PubMed:28733419). Induced in fruit by storage in cold (PubMed:29528226). Induced by hydrogen peroxide and infection with root-knot nematodes (PubMed:30576511). {ECO:0000269\|PubMed:15231736, ECO:0000269\|PubMed:28733419, ECO:0000269\|PubMed:29528226, ECO:0000269\|PubMed:30576511}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	LN681860	0.0	LN681860.1 Cucumis melo genomic scaffold, anchoredscaffold00021.
GenBank	LN713260	0.0	LN713260.1 Cucumis melo genomic chromosome, chr_6.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_004148739.1	0.0	PREDICTED: transcription factor MYC2-like
Swissprot	A0A3Q7HRZ6	1e-120	MYC2_SOLLC; Transcription factor MYC2
TrEMBL	A0A0A0L3Z0	0.0	A0A0A0L3Z0_CUCSA; Transcription factor AtMYC2
STRING	XP_004164415.1	0.0	(Cucumis sativus)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF2938	33	64

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G17880.1	2e-90	bHLH family protein

Link Out ? help Back to Top
Phytozome	Cucsa.321840.1
Entrez Gene	101205372

Publications ? help Back to Top
Ren Y, et al. An integrated genetic and cytogenetic map of the cucumber genome. PLoS ONE, 2009. 4(6): p. e5795 [PMID:19495411] Guo S, et al. Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types. BMC Genomics, 2010. 11: p. 384 [PMID:20565788] Li Z, et al. RNA-Seq improves annotation of protein-coding genes in the cucumber genome. BMC Genomics, 2011. 12: p. 540 [PMID:22047402] Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Gupta N,Prasad VB,Chattopadhyay S LeMYC2 acts as a negative regulator of blue light mediated photomorphogenic growth, and promotes the growth of adult tomato plants. BMC Plant Biol., 2014. 14: p. 38 [PMID:24483714] Sun H,Fan HJ,Ling HQ Genome-wide identification and characterization of the bHLH gene family in tomato. BMC Genomics, 2015. 16: p. 9 [PMID:25612924] Du M, et al. MYC2 Orchestrates a Hierarchical Transcriptional Cascade That Regulates Jasmonate-Mediated Plant Immunity in Tomato. Plant Cell, 2017. 29(8): p. 1883-1906 [PMID:28733419] Min D, et al. SlMYC2 Involved in Methyl Jasmonate-Induced Tomato Fruit Chilling Tolerance. J. Agric. Food Chem., 2018. 66(12): p. 3110-3117 [PMID:29528226] Xu X, et al. Strigolactones positively regulate defense against root-knot nematodes in tomato. J. Exp. Bot., 2019. 70(4): p. 1325-1337 [PMID:30576511] Liu Y, et al. MYC2 Regulates the Termination of Jasmonate Signaling via an Autoregulatory Negative Feedback Loop. Plant Cell, 2019. 31(1): p. 106-127 [PMID:30610166]