PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis

Family

AP2

Protein Properties

Length: 538aa MW: 59457.6 Da PI: 7.2847

Description

AP2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Cucsa.102550.1	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	53.3	7e-17	179	228	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrdpsengkr.krfslgkfgtaeeAakaaiaarkkleg 55 s+y+GV++++++grW+++I+d + k+++lg f+ta Aa+a+++a+ k++g Cucsa.102550.1 179 SQYRGVTFYRRTGRWESHIWD------CgKQVYLGGFDTAHAAARAYDRAAIKFRG 228 78*****************......55**********************997 PP
2	AP2	43.7	6.9e-14	271	321	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55 s+y+GV+ +k grW+A+ + +k+++lg f+t+ eAa+a+++a+ k +g Cucsa.102550.1 271 SKYRGVTLHK-CGRWEARMGQF--L-GKKYVYLGLFDTEIEAARAYDKAAIKCNG 321 89******.7**5553..2.26******99********99776 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF00847	6.4E-9	179	228	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	1.11E-15	179	237	IPR016177	DNA-binding domain
Gene3D	G3DSA:3.30.730.10	6.2E-17	180	236	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	17.701	180	236	IPR001471	AP2/ERF domain
CDD	cd00018	3.64E-11	180	235	No hit	No description
SMART	SM00380	1.9E-31	180	242	IPR001471	AP2/ERF domain
CDD	cd00018	2.71E-25	271	331	No hit	No description
Pfam	PF00847	1.5E-9	271	321	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	6.41E-17	271	331	IPR016177	DNA-binding domain
Gene3D	G3DSA:3.30.730.10	2.5E-16	272	329	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	15.461	272	329	IPR001471	AP2/ERF domain
SMART	SM00380	4.2E-31	272	335	IPR001471	AP2/ERF domain
PRINTS	PR00367	5.3E-6	273	284	IPR001471	AP2/ERF domain
PRINTS	PR00367	5.3E-6	311	331	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 538 aa Download sequence Send to blast
MGCDAFSRLT KTSPPNKTKT KILLTQTSFP KGTRKKKMWD LNDWPDVREE DECSSAKTSI 60 EGEGDEEKGK RVGSLSNSSS SAVVMEEEEA EVEGGSDEEE PTPMVTHQFF PLEETEIPTP 120 LPHASAPPAT APAFPRAHWV GVKFAHPDPL AALPNNSLTP TDLSHPIKKS RRGPRSRSSQ 180 YRGVTFYRRT GRWESHIWDC GKQVYLGGFD TAHAAARAYD RAAIKFRGVE ADINFSIEDY 240 EDDLKQMGNL TKEEFVHVLR RQSTGFPRGS SKYRGVTLHK CGRWEARMGQ FLGKKYVYLG 300 LFDTEIEAAR AYDKAAIKCN GKEAVTNFDP SIYENELNPT TESSSNLGDH SLDLSLGNSS 360 SKQNDSSNNG SIGPQHHSSS SSSADWQRNH GFRPLQLNLE NGGGGNKNYN NNVERRNRYL 420 ESETMQLLSQ THIQSPAQFS RPHTVGGAPD HHPHSQILLH NHNNQTFNYQ IEFSGSSNGH 480 GGRIVGSDLT LSLNDHLQSG PSQVLASAAA SSGFAPQIRP SKNWLHNNGF HCLMRPS*

Sequence ? help Back to Top

Protein Sequence Length: 538 aa Download sequence Send to blast

MGCDAFSRLT KTSPPNKTKT KILLTQTSFP KGTRKKKMWD LNDWPDVREE DECSSAKTSI  60
EGEGDEEKGK RVGSLSNSSS SAVVMEEEEA EVEGGSDEEE PTPMVTHQFF PLEETEIPTP  120
LPHASAPPAT APAFPRAHWV GVKFAHPDPL AALPNNSLTP TDLSHPIKKS RRGPRSRSSQ  180
YRGVTFYRRT GRWESHIWDC GKQVYLGGFD TAHAAARAYD RAAIKFRGVE ADINFSIEDY  240
EDDLKQMGNL TKEEFVHVLR RQSTGFPRGS SKYRGVTLHK CGRWEARMGQ FLGKKYVYLG  300
LFDTEIEAAR AYDKAAIKCN GKEAVTNFDP SIYENELNPT TESSSNLGDH SLDLSLGNSS  360
SKQNDSSNNG SIGPQHHSSS SSSADWQRNH GFRPLQLNLE NGGGGNKNYN NNVERRNRYL  420
ESETMQLLSQ THIQSPAQFS RPHTVGGAPD HHPHSQILLH NHNNQTFNYQ IEFSGSSNGH  480
GGRIVGSDLT LSLNDHLQSG PSQVLASAAA SSGFAPQIRP SKNWLHNNGF HCLMRPS*

Functional Description ? help Back to Top
Source	Description
UniProt	Probable transcriptional activator that promotes early floral meristem identity (PubMed:7919989). Is required subsequently for the transition of an inflorescence meristem into a floral meristem (PubMed:1675158). Plays a central role in the specification of floral identity, particularly for the normal development of sepals and petals in the wild-type flower, by spatially controlling the expression domains of multiple floral organ identity genes (PubMed:1675158, PubMed:23034631). Acts as A class cadastral protein by repressing the C class floral homeotic gene AGAMOUS in association with other repressors like LEUNIG and SEUSS (PubMed:1675158). Directly represses AGAMOUS by recruiting the transcriptional corepressor TOPLESS and the histone deacetylase HDA19 (PubMed:23034631). It is also required during seed development (PubMed:1675158). {ECO:0000269\|PubMed:1675158, ECO:0000269\|PubMed:23034631, ECO:0000269\|PubMed:7919989}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Negatively regulated by the C class floral homeotic protein AGAMOUS in stamens and carpels. MicroRNA 172 (miRNA172) negatively regulates APETALA2 at the translational level and may modulate its expression pattern. Seems not to be influenced by jasmonate and Alternaria brassicicola. {ECO:0000269\|PubMed:12805630, ECO:0000269\|PubMed:12893888, ECO:0000269\|PubMed:14555699}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	LN681875	0.0	LN681875.1 Cucumis melo genomic scaffold, anchoredscaffold00007.
GenBank	LN713262	0.0	LN713262.1 Cucumis melo genomic chromosome, chr_8.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_004141908.1	0.0	PREDICTED: AP2-like ethylene-responsive transcription factor TOE3
Swissprot	P47927	1e-123	AP2_ARATH; Floral homeotic protein APETALA 2
TrEMBL	A0A0A0KGD2	0.0	A0A0A0KGD2_CUCSA; Uncharacterized protein
STRING	XP_004141908.1	0.0	(Cucumis sativus)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF3784	34	63

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G36920.2	1e-105	AP2 family protein

Link Out ? help Back to Top
Phytozome	Cucsa.102550.1
Entrez Gene	101214724

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ren Y, et al. An integrated genetic and cytogenetic map of the cucumber genome. PLoS ONE, 2009. 4(6): p. e5795 [PMID:19495411] Guo S, et al. Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types. BMC Genomics, 2010. 11: p. 384 [PMID:20565788] Li Z, et al. RNA-Seq improves annotation of protein-coding genes in the cucumber genome. BMC Genomics, 2011. 12: p. 540 [PMID:22047402] Thamilarasan SK,Park JI,Jung HJ,Nou IS Genome-wide analysis of the distribution of AP2/ERF transcription factors reveals duplication and CBFs genes elucidate their potential function in Brassica oleracea. BMC Genomics, 2014. 15: p. 422 [PMID:24888752] Zhang GB,Yi HY,Gong JM The Arabidopsis ethylene/jasmonic acid-NRT signaling module coordinates nitrate reallocation and the trade-off between growth and environmental adaptation. Plant Cell, 2014. 26(10): p. 3984-98 [PMID:25326291] Ranocha P,Francoz E,Burlat V,Dunand C Expression of PRX36, PMEI6 and SBT1.7 is controlled by complex transcription factor regulatory networks for proper seed coat mucilage extrusion. Plant Signal Behav, 2014. 9(11): p. e977734 [PMID:25531128] Djemal R,Khoudi H Isolation and molecular characterization of a novel WIN1/SHN1 ethylene-responsive transcription factor TdSHN1 from durum wheat (Triticum turgidum. L. subsp. durum). Protoplasma, 2015. 252(6): p. 1461-73 [PMID:25687296] Kazan K Diverse roles of jasmonates and ethylene in abiotic stress tolerance. Trends Plant Sci., 2015. 20(4): p. 219-29 [PMID:25731753] Prunet N, et al. SQUINT promotes stem cell homeostasis and floral meristem termination in Arabidopsis through APETALA2 and CLAVATA signalling. J. Exp. Bot., 2015. 66(21): p. 6905-16 [PMID:26269626] Xie W, et al. Exploring potential new floral organ morphogenesis genes of Arabidopsis thaliana using systems biology approach. Front Plant Sci, 2015. 6: p. 829 [PMID:26528302] Zumajo-Cardona C,Pabón-Mora N Evolution of the APETALA2 Gene Lineage in Seed Plants. Mol. Biol. Evol., 2016. 33(7): p. 1818-32 [PMID:27030733] Zhao Y, et al. An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design. Sci Rep, 2016. 6: p. 23890 [PMID:27033976] Gao R,Liu P,Irwanto N,Loh R,Wong SM Upregulation of LINC-AP2 is negatively correlated with AP2 gene expression with Turnip crinkle virus infection in Arabidopsis thaliana. Plant Cell Rep., 2016. 35(11): p. 2257-2267 [PMID:27473526] Huang Z, et al. APETALA2 antagonizes the transcriptional activity of AGAMOUS in regulating floral stem cells in Arabidopsis thaliana. New Phytol., 2017. 215(3): p. 1197-1209 [PMID:27604611] Dory M, et al. Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo. BMC Plant Biol., 2016. 16(1): p. 204 [PMID:27655033] Wang P, et al. Expansion and Functional Divergence of AP2 Group Genes in Spermatophytes Determined by Molecular Evolution and Arabidopsis Mutant Analysis. Front Plant Sci, 2016. 7: p. 1383 [PMID:27703459] Sharma P, et al. Promoter Trapping and Deletion Analysis Show Arabidopsis thaliana APETALA2 Gene Promoter Is Bidirectional and Functions as a Pollen- and Ovule-Specific Promoter in the Reverse Orientation. Appl. Biochem. Biotechnol., 2017. 182(4): p. 1591-1604 [PMID:28130768] Kihira M, et al. Arabidopsis thaliana FLO2 is Involved in Efficiency of Photoassimilate Translocation, Which is Associated with Leaf Growth and Aging, Yield of Seeds and Seed Quality. Plant Cell Physiol., 2017. 58(3): p. 440-450 [PMID:28158741] Balanzà V, et al. Genetic control of meristem arrest and life span in Arabidopsis by a FRUITFULL-APETALA2 pathway. Nat Commun, 2018. 9(1): p. 565 [PMID:29422669] Dotto M,Gómez MS,Soto MS,Casati P UV-B radiation delays flowering time through changes in the PRC2 complex activity and miR156 levels in Arabidopsis thaliana. Plant Cell Environ., 2018. 41(6): p. 1394-1406 [PMID:29447428] Song C,Lee J,Kim T,Hong JC,Lim CO VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis. Planta, 2018. 247(6): p. 1439-1448 [PMID:29536220]