PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Citrullus

Family

AP2

Protein Properties

Length: 501aa MW: 55741.6 Da PI: 5.2508

Description

AP2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Cla020243	genome	ICuGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	54.1	3.9e-17	141	190	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrdpsengkr.krfslgkfgtaeeAakaaiaarkkleg 55 s+y+GV++++++grW+++I+d + k+++lg f+ta Aa+a+++a+ k++g Cla020243 141 SQYRGVTFYRRTGRWESHIWD------CgKQVYLGGFDTAHAAARAYDRAAIKFRG 190 78*****************......55**********************998 PP
2	AP2	28.4	4e-09	233	273	1	45
AP2 1 sgykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAaka 45 s+++GV+ +k grW+A+ + +k+++lg f+++ eAa++ Cla020243 233 SKFRGVTLHK-CGRWEARMGQF--L-GKKYVYLGLFDSEIEAARL 273 79******.7**5553..2.26******99*97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF54171	6.28E-16	141	199	IPR016177	DNA-binding domain
Pfam	PF00847	2.9E-9	141	190	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	1.5E-17	142	198	IPR001471	AP2/ERF domain
SMART	SM00380	1.9E-32	142	204	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	17.754	142	198	IPR001471	AP2/ERF domain
CDD	cd00018	6.28E-12	142	197	No hit	No description
SuperFamily	SSF54171	1.37E-10	233	308	IPR016177	DNA-binding domain
CDD	cd00018	1.05E-20	233	308	No hit	No description
Pfam	PF00847	3.4E-5	233	273	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	4.8E-8	234	306	IPR001471	AP2/ERF domain
SMART	SM00380	9.8E-22	234	312	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	8.729	234	275	IPR001471	AP2/ERF domain
PRINTS	PR00367	1.5E-5	235	246	IPR001471	AP2/ERF domain
PRINTS	PR00367	1.5E-5	288	308	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 501 aa Download sequence Send to blast
MWDLNDSPDY GGTDEFEVLS SIDGDEDRAK WIGSINSNSS SSVVVMEDGS DADEASVGED 60 EPLVHRKNFS VTHPLATRQF FPLEDSDVGA SSAAVGGSAT FPRARWVGVK FCQTEPVAAV 120 RPMAVLQPIK KSRRGPRSRS SQYRGVTFYR RTGRWESHIW DCGKQVYLGG FDTAHAAARA 180 YDRAAIKFRG SEADINFSIE DYEEDLQQMG NLTKEEFVHV LRRQSTGYPR GTSKFRGVTL 240 HKCGRWEARM GQFLGKKYVY LGLFDSEIEA ARLPDYELQL VSATCFRAYD KAAIKCNGKE 300 AVTNFDPSIY EDELSTTESS SAKVWEQNLD LRLGNSSSKS HTLSFGNHCP NVTPNIDLQI 360 TNESNPQESN IFENDNGGSC TFLQTEKMQF RSEMIVRSPP SVEMMKHGRL ETPMELQTLH 420 NYSPQINQSN SQIQLLSSSN EGGLGSDELS LCLSEGHQWQ QSGGSQQFAN AAASSGFPQL 480 QISTSQNWLQ KNNGCFLQRP S

Sequence ? help Back to Top

Protein Sequence Length: 501 aa Download sequence Send to blast

MWDLNDSPDY GGTDEFEVLS SIDGDEDRAK WIGSINSNSS SSVVVMEDGS DADEASVGED  60
EPLVHRKNFS VTHPLATRQF FPLEDSDVGA SSAAVGGSAT FPRARWVGVK FCQTEPVAAV  120
RPMAVLQPIK KSRRGPRSRS SQYRGVTFYR RTGRWESHIW DCGKQVYLGG FDTAHAAARA  180
YDRAAIKFRG SEADINFSIE DYEEDLQQMG NLTKEEFVHV LRRQSTGYPR GTSKFRGVTL  240
HKCGRWEARM GQFLGKKYVY LGLFDSEIEA ARLPDYELQL VSATCFRAYD KAAIKCNGKE  300
AVTNFDPSIY EDELSTTESS SAKVWEQNLD LRLGNSSSKS HTLSFGNHCP NVTPNIDLQI  360
TNESNPQESN IFENDNGGSC TFLQTEKMQF RSEMIVRSPP SVEMMKHGRL ETPMELQTLH  420
NYSPQINQSN SQIQLLSSSN EGGLGSDELS LCLSEGHQWQ QSGGSQQFAN AAASSGFPQL  480
QISTSQNWLQ KNNGCFLQRP S

Functional Description ? help Back to Top
Source	Description
UniProt	Probable transcriptional activator that promotes early floral meristem identity (PubMed:7919989). Is required subsequently for the transition of an inflorescence meristem into a floral meristem (PubMed:1675158). Plays a central role in the specification of floral identity, particularly for the normal development of sepals and petals in the wild-type flower, by spatially controlling the expression domains of multiple floral organ identity genes (PubMed:1675158, PubMed:23034631). Acts as A class cadastral protein by repressing the C class floral homeotic gene AGAMOUS in association with other repressors like LEUNIG and SEUSS (PubMed:1675158). Directly represses AGAMOUS by recruiting the transcriptional corepressor TOPLESS and the histone deacetylase HDA19 (PubMed:23034631). It is also required during seed development (PubMed:1675158). {ECO:0000269\|PubMed:1675158, ECO:0000269\|PubMed:23034631, ECO:0000269\|PubMed:7919989}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Negatively regulated by the C class floral homeotic protein AGAMOUS in stamens and carpels. MicroRNA 172 (miRNA172) negatively regulates APETALA2 at the translational level and may modulate its expression pattern. Seems not to be influenced by jasmonate and Alternaria brassicicola. {ECO:0000269\|PubMed:12805630, ECO:0000269\|PubMed:12893888, ECO:0000269\|PubMed:14555699}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	LN681913	1e-169	LN681913.1 Cucumis melo genomic scaffold, anchoredscaffold00045.
GenBank	LN713265	1e-169	LN713265.1 Cucumis melo genomic chromosome, chr_11.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_008457743.1	0.0	PREDICTED: AP2-like ethylene-responsive transcription factor TOE3 isoform X1
Refseq	XP_008457744.1	0.0	PREDICTED: AP2-like ethylene-responsive transcription factor TOE3 isoform X1
Swissprot	P47927	1e-115	AP2_ARATH; Floral homeotic protein APETALA 2
TrEMBL	A0A1S3C676	0.0	A0A1S3C676_CUCME; AP2-like ethylene-responsive transcription factor TOE3 isoform X1
STRING	XP_008457743.1	0.0	(Cucumis melo)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF3784	34	63

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G36920.2	1e-109	AP2 family protein

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Thamilarasan SK,Park JI,Jung HJ,Nou IS Genome-wide analysis of the distribution of AP2/ERF transcription factors reveals duplication and CBFs genes elucidate their potential function in Brassica oleracea. BMC Genomics, 2014. 15: p. 422 [PMID:24888752] Zhang GB,Yi HY,Gong JM The Arabidopsis ethylene/jasmonic acid-NRT signaling module coordinates nitrate reallocation and the trade-off between growth and environmental adaptation. Plant Cell, 2014. 26(10): p. 3984-98 [PMID:25326291] Ranocha P,Francoz E,Burlat V,Dunand C Expression of PRX36, PMEI6 and SBT1.7 is controlled by complex transcription factor regulatory networks for proper seed coat mucilage extrusion. Plant Signal Behav, 2014. 9(11): p. e977734 [PMID:25531128] Djemal R,Khoudi H Isolation and molecular characterization of a novel WIN1/SHN1 ethylene-responsive transcription factor TdSHN1 from durum wheat (Triticum turgidum. L. subsp. durum). Protoplasma, 2015. 252(6): p. 1461-73 [PMID:25687296] Kazan K Diverse roles of jasmonates and ethylene in abiotic stress tolerance. Trends Plant Sci., 2015. 20(4): p. 219-29 [PMID:25731753] Prunet N, et al. SQUINT promotes stem cell homeostasis and floral meristem termination in Arabidopsis through APETALA2 and CLAVATA signalling. J. Exp. Bot., 2015. 66(21): p. 6905-16 [PMID:26269626] Xie W, et al. Exploring potential new floral organ morphogenesis genes of Arabidopsis thaliana using systems biology approach. Front Plant Sci, 2015. 6: p. 829 [PMID:26528302] Zumajo-Cardona C,Pabón-Mora N Evolution of the APETALA2 Gene Lineage in Seed Plants. Mol. Biol. Evol., 2016. 33(7): p. 1818-32 [PMID:27030733] Zhao Y, et al. An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design. Sci Rep, 2016. 6: p. 23890 [PMID:27033976] Gao R,Liu P,Irwanto N,Loh R,Wong SM Upregulation of LINC-AP2 is negatively correlated with AP2 gene expression with Turnip crinkle virus infection in Arabidopsis thaliana. Plant Cell Rep., 2016. 35(11): p. 2257-2267 [PMID:27473526] Huang Z, et al. APETALA2 antagonizes the transcriptional activity of AGAMOUS in regulating floral stem cells in Arabidopsis thaliana. New Phytol., 2017. 215(3): p. 1197-1209 [PMID:27604611] Dory M, et al. Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo. BMC Plant Biol., 2016. 16(1): p. 204 [PMID:27655033] Wang P, et al. Expansion and Functional Divergence of AP2 Group Genes in Spermatophytes Determined by Molecular Evolution and Arabidopsis Mutant Analysis. Front Plant Sci, 2016. 7: p. 1383 [PMID:27703459] Sharma P, et al. Promoter Trapping and Deletion Analysis Show Arabidopsis thaliana APETALA2 Gene Promoter Is Bidirectional and Functions as a Pollen- and Ovule-Specific Promoter in the Reverse Orientation. Appl. Biochem. Biotechnol., 2017. 182(4): p. 1591-1604 [PMID:28130768] Kihira M, et al. Arabidopsis thaliana FLO2 is Involved in Efficiency of Photoassimilate Translocation, Which is Associated with Leaf Growth and Aging, Yield of Seeds and Seed Quality. Plant Cell Physiol., 2017. 58(3): p. 440-450 [PMID:28158741] Balanzà V, et al. Genetic control of meristem arrest and life span in Arabidopsis by a FRUITFULL-APETALA2 pathway. Nat Commun, 2018. 9(1): p. 565 [PMID:29422669] Dotto M,Gómez MS,Soto MS,Casati P UV-B radiation delays flowering time through changes in the PRC2 complex activity and miR156 levels in Arabidopsis thaliana. Plant Cell Environ., 2018. 41(6): p. 1394-1406 [PMID:29447428] Song C,Lee J,Kim T,Hong JC,Lim CO VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis. Planta, 2018. 247(6): p. 1439-1448 [PMID:29536220]