PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus

Family

WRKY

Protein Properties

Length: 554aa MW: 60623.8 Da PI: 6.5843

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Ciclev10007904m	genome	ICGC	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	102.6	2.2e-32	134	191	2	60
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 +DgynWrKYGqK+vkgse+prsYY+Ct+++C+vkkkvers +++++ei+Y+g Hnh+k Ciclev10007904m 134 EDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCQVKKKVERSL-EGHITEIIYKGAHNHPK 191 7**************************************.*************85 PP
2	WRKY	104.2	7e-33	346	404	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+vkg+++prsYY+CtsagC+v+k+ver+++d k v++tYeg+Hnh+ Ciclev10007904m 346 LDDGYRWRKYGQKVVKGNPNPRSYYKCTSAGCTVRKHVERASHDLKSVITTYEGKHNHD 404 59********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	22.848	128	192	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	2.6E-28	132	192	IPR003657	WRKY domain
SMART	SM00774	6.8E-36	133	191	IPR003657	WRKY domain
SuperFamily	SSF118290	1.83E-25	133	192	IPR003657	WRKY domain
Pfam	PF03106	1.1E-24	134	190	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	3.7E-37	331	406	IPR003657	WRKY domain
SuperFamily	SSF118290	1.11E-28	338	406	IPR003657	WRKY domain
PROSITE profile	PS50811	38.664	341	406	IPR003657	WRKY domain
SMART	SM00774	1.5E-37	346	405	IPR003657	WRKY domain
Pfam	PF03106	4.6E-25	347	404	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009555	Biological Process	pollen development
GO:0009942	Biological Process	longitudinal axis specification
GO:0030010	Biological Process	establishment of cell polarity
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 554 aa Download sequence Send to blast
MAGFLMLFVK HFHFLSVEYC LTSTYRFTFT HPQQSFPSIE VSVQSENSPQ NVNAAKVHSQ 60 YRNSLSLQVQ TDFSRATTEK DNGCNTILAD QRVFDTFGGS TEHSPPLDEQ QDEEGDQRGS 120 GDSMVAGAGG ASSEDGYNWR KYGQKQVKGS EYPRSYYKCT HPNCQVKKKV ERSLEGHITE 180 IIYKGAHNHP KPPPNRRSAI GSNPLDMQLD IHEQAGLQSG ADGDPSWANT QKGTAGGTPE 240 WRHDTVEVTS AAPVGPEYCN QSSALPAQNG TPYESGDAVD ASSTFSNDED EDDQVTHCSV 300 GFDGEEDESE SKRRKIEAYA TEMSGATRAI REPRVVVQTT SEVDILDDGY RWRKYGQKVV 360 KGNPNPRSYY KCTSAGCTVR KHVERASHDL KSVITTYEGK HNHDVPAARN SSHVNSGSSS 420 AVSAHAAAGG QIHRPEPSQL HNSMARFDRP ASFGSFRVPG RQQFGPSPGF SFGMHQPGLA 480 NLSMAGLGPG QPELPVLPVH PYLAQQRQVN EMGFMMPKGE PKVEPMSEPG LNLSNRSAVY 540 QQLMSRLPLG PQM*

Sequence ? help Back to Top

Protein Sequence Length: 554 aa Download sequence Send to blast

MAGFLMLFVK HFHFLSVEYC LTSTYRFTFT HPQQSFPSIE VSVQSENSPQ NVNAAKVHSQ  60
YRNSLSLQVQ TDFSRATTEK DNGCNTILAD QRVFDTFGGS TEHSPPLDEQ QDEEGDQRGS  120
GDSMVAGAGG ASSEDGYNWR KYGQKQVKGS EYPRSYYKCT HPNCQVKKKV ERSLEGHITE  180
IIYKGAHNHP KPPPNRRSAI GSNPLDMQLD IHEQAGLQSG ADGDPSWANT QKGTAGGTPE  240
WRHDTVEVTS AAPVGPEYCN QSSALPAQNG TPYESGDAVD ASSTFSNDED EDDQVTHCSV  300
GFDGEEDESE SKRRKIEAYA TEMSGATRAI REPRVVVQTT SEVDILDDGY RWRKYGQKVV  360
KGNPNPRSYY KCTSAGCTVR KHVERASHDL KSVITTYEGK HNHDVPAARN SSHVNSGSSS  420
AVSAHAAAGG QIHRPEPSQL HNSMARFDRP ASFGSFRVPG RQQFGPSPGF SFGMHQPGLA  480
NLSMAGLGPG QPELPVLPVH PYLAQQRQVN EMGFMMPKGE PKVEPMSEPG LNLSNRSAVY  540
QQLMSRLPLG PQM*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	1e-36	134	407	8	78	Probable WRKY transcription factor 4
2lex_A	1e-36	134	407	8	78	Probable WRKY transcription factor 4
Search in ModeBase

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Low expression in senescent leaves (PubMed:11722756). Expressed in both the unfertilized egg cell and the pollen tube (PubMed:21316593). {ECO:0000269\|PubMed:11722756, ECO:0000269\|PubMed:21316593}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Regulates WOX8 and WOX9 expression and basal cell division patterns during early embryogenesis. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Required to repolarize the zygote from a transient symmetric state. {ECO:0000269\|PubMed:21316593}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00071	PBM	Transfer from AT5G56270	Download

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_024033318.1	0.0	probable WRKY transcription factor 2 isoform X1
Refseq	XP_024033319.1	0.0	probable WRKY transcription factor 2 isoform X1
Swissprot	Q9FG77	1e-149	WRKY2_ARATH; Probable WRKY transcription factor 2
TrEMBL	V4UTU4	0.0	V4UTU4_9ROSI; Uncharacterized protein
STRING	XP_006452781.1	0.0	(Citrus clementina)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM7954	27	40

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G56270.1	1e-148	WRKY DNA-binding protein 2

Link Out ? help Back to Top
Phytozome	Ciclev10007904m
Entrez Gene	18054799

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ueda M, et al. Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote. Genes Dev., 2017. 31(6): p. 617-627 [PMID:28404632] Lei R, et al. Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function. Plant J., 2017. 91(6): p. 962-976 [PMID:28635025]