PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Capsella

Family

WRKY

Protein Properties

Length: 324aa MW: 36047.4 Da PI: 7.0088

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Carubv10005249m	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	108	4.6e-34	175	232	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 +Dgy+WrKYGqK vk+s++prsYYrCt+++C+vkk+vers +dp+vv++tYeg+Hnh+ Carubv10005249m 175 EDGYRWRKYGQKAVKNSPYPRSYYRCTTQRCNVKKRVERSFQDPTVVITTYEGQHNHP 232 8********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PIRSF	PIRSF038130	6.6E-113	1	323	IPR017396	PWRKY transcription factor, group IIc
Gene3D	G3DSA:2.20.25.80	1.3E-35	159	234	IPR003657	WRKY domain
SuperFamily	SSF118290	1.28E-29	166	234	IPR003657	WRKY domain
PROSITE profile	PS50811	32.856	169	234	IPR003657	WRKY domain
SMART	SM00774	1.8E-39	174	233	IPR003657	WRKY domain
Pfam	PF03106	2.4E-26	175	232	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 324 aa Download sequence Send to blast
MSNETRDLYN YQYPPSFSLH EMMNLPTSNL PSYGNIISSQ NNGFDPSSNY SFTDCLQSSP 60 AAYESILQKT FGLSPSSSEV FNSSIDQESN RDVTNDVING SGACNETTTR VSASSSSSEA 120 DHPGEDSGKS NRKRELVGEE DQSSKKVGKT KKNELKKQRE PRVSFMTKSE VDHLEDGYRW 180 RKYGQKAVKN SPYPRSYYRC TTQRCNVKKR VERSFQDPTV VITTYEGQHN HPIPTNLRGS 240 SAAAAMFSAD LMTPRSFAHD MFRTAAYASG GPAGAALDYG YGQNGYGNVN ANPSSHQDQY 300 HHGGDQYELL REIFPSMFFK QEP*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	2e-29	165	234	8	77	Probable WRKY transcription factor 4
2lex_A	2e-29	165	234	8	77	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00440	DAP	Transfer from AT4G18170	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Carubv10005249m

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF442393	0.0	AF442393.1 Arabidopsis thaliana WRKY transcription factor 28 (WRKY28) mRNA, complete cds.
GenBank	AY070761	0.0	AY070761.1 Arabidopsis thaliana AT4g18170/T9A21_10 mRNA, complete cds.
GenBank	AY116943	0.0	AY116943.1 Arabidopsis thaliana AT4g18170/T9A21_10 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006284116.1	0.0	probable WRKY transcription factor 28
Swissprot	Q8VWJ2	0.0	WRK28_ARATH; WRKY transcription factor 28
TrEMBL	R0GJI0	0.0	R0GJI0_9BRAS; WRKY transcription factor
STRING	XP_006284116.1	0.0	(Capsella rubella)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1524	26	93

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G18170.1	1e-179	WRKY DNA-binding protein 28

Link Out ? help Back to Top
Phytozome	Carubv10005249m
Entrez Gene	17879048

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Kim SH, et al. Characterization of a Novel DWD protein that participates in heat stress response in Arabidopsis. Mol. Cells, 2014. 37(11): p. 833-40 [PMID:25358503] Zhao L, et al. KLU suppresses megasporocyte cell fate through SWR1-mediated activation of WRKY28 expression in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2018. 115(3): p. E526-E535 [PMID:29288215]