PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Capsella

Family

MIKC_MADS

Protein Properties

Length: 292aa MW: 33427.9 Da PI: 9.9372

Description

MIKC_MADS family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Cagra.9536s0004.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	SRF-TF	99.5	1.3e-31	64	114	1	51
S---SHHHHHHHHHHHHHHHHHHHHHHHHHHT-EEEEEEE-TTSEEEEEE- CS SRF-TF 1 krienksnrqvtfskRrngilKKAeELSvLCdaevaviifsstgklyeyss 51 krien++nrqvtf+kRrng+lKKA+ELSvLCdaeva+i+fss+g+lyeys+ Cagra.9536s0004.1.p 64 KRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEYSN 114 79***********************************************95 PP
2	K-box	108.5	7.6e-36	131	228	3	100
K-box 3 kssgksleeakaeslqqelakLkkeienLqreqRhllGedLesLslkeLqqLeqqLekslkkiRskKnellleqieelqkkekelqeenk 92 +s++ s++e +a+++qqe+akL+++i ++q+++R+l+Ge ++s+s keL++Le +Le+s+++iRskKnell+++i+++qk+e +l+++n+ Cagra.9536s0004.1.p 131 NSNTGSVAEINAQYYQQESAKLRQQIISIQNSNRQLMGETIGSMSPKELRNLEGRLERSITRIRSKKNELLFSEIDYMQKREVDLHNDNQ 220 45555699******************************************************************************** PP K-box 93 aLrkklee 100 Lr+k++e Cagra.9536s0004.1.p 221 ILRAKIAE 228 ***986 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00432	1.3E-40	56	115	IPR002100	Transcription factor, MADS-box
PROSITE profile	PS50066	33.221	56	116	IPR002100	Transcription factor, MADS-box
CDD	cd00265	1.07E-44	57	130	No hit	No description
SuperFamily	SSF55455	4.19E-33	57	129	IPR002100	Transcription factor, MADS-box
PRINTS	PR00404	9.5E-33	58	78	IPR002100	Transcription factor, MADS-box
PROSITE pattern	PS00350	0	58	112	IPR002100	Transcription factor, MADS-box
Pfam	PF00319	1.2E-26	65	112	IPR002100	Transcription factor, MADS-box
PRINTS	PR00404	9.5E-33	78	93	IPR002100	Transcription factor, MADS-box
PRINTS	PR00404	9.5E-33	93	114	IPR002100	Transcription factor, MADS-box
Pfam	PF01486	3.5E-27	141	226	IPR002487	Transcription factor, K-box
PROSITE profile	PS51297	15.276	142	232	IPR002487	Transcription factor, K-box

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0048366	Biological Process	leaf development
GO:0048440	Biological Process	carpel development
GO:0048443	Biological Process	stamen development
GO:0048497	Biological Process	maintenance of floral organ identity
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 292 aa Download sequence Send to blast
MHNLTFHFLQ LLQISYFPEN HFPKKNKTFL FVLLPPTAIT AYQAELGEDS SPLRKSGRGK 60 IEIKRIENTT NRQVTFCKRR NGLLKKAYEL SVLCDAEVAL IVFSSRGRLY EYSNNSVKGT 120 IERYKKAISD NSNTGSVAEI NAQYYQQESA KLRQQIISIQ NSNRQLMGET IGSMSPKELR 180 NLEGRLERSI TRIRSKKNEL LFSEIDYMQK REVDLHNDNQ ILRAKIAENE RNNPSISLMP 240 GGSNYEQLMP PPQTQPQPFD SRNYFQVAAL QPNNHHYSSA GRQDQTALQL V*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1egw_A	1e-19	57	124	1	68	MADS BOX TRANSCRIPTION ENHANCER FACTOR 2, POLYPEPTIDE A
1egw_B	1e-19	57	124	1	68	MADS BOX TRANSCRIPTION ENHANCER FACTOR 2, POLYPEPTIDE A
1egw_C	1e-19	57	124	1	68	MADS BOX TRANSCRIPTION ENHANCER FACTOR 2, POLYPEPTIDE A
1egw_D	1e-19	57	124	1	68	MADS BOX TRANSCRIPTION ENHANCER FACTOR 2, POLYPEPTIDE A
1tqe_P	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
1tqe_Q	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
1tqe_R	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
1tqe_S	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
3p57_A	2e-19	57	124	1	68	Myocyte-specific enhancer factor 2A
3p57_B	2e-19	57	124	1	68	Myocyte-specific enhancer factor 2A
3p57_C	2e-19	57	124	1	68	Myocyte-specific enhancer factor 2A
3p57_D	2e-19	57	124	1	68	Myocyte-specific enhancer factor 2A
3p57_I	2e-19	57	124	1	68	Myocyte-specific enhancer factor 2A
3p57_J	2e-19	57	124	1	68	Myocyte-specific enhancer factor 2A
6c9l_A	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
6c9l_B	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
6c9l_C	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
6c9l_D	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
6c9l_E	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
6c9l_F	2e-19	57	124	2	69	Myocyte-specific enhancer factor 2B
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Probable transcription factor involved in the control of organ identity during the early development of flowers. Is required for normal development of stamens and carpels in the wild-type flower. Plays a role in maintaining the determinacy of the floral meristem. Acts as C class cadastral protein by repressing the A class floral homeotic genes like APETALA1. Forms a heterodimer via the K-box domain with either SEPALATTA1/AGL2, SEPALATTA2/AGL4, SEPALLATA3/AGL9 or AGL6 that could be involved in genes regulation during floral meristem development. Controls AHL21/GIK, a multifunctional chromatin modifier in reproductive organ patterning and differentiation (PubMed:19956801). Induces microsporogenesis through the activation of SPL/NZZ (PubMed:15254538). {ECO:0000269\|PubMed:15254538, ECO:0000269\|PubMed:19956801}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00609	ChIP-seq	Transfer from AT4G18960	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Cagra.9536s0004.1.p

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Negatively regulated by the A class floral homeotic protein APETALA2 and by other repressors like LEUNIG, SEUSS, SAP or CURLY LEAF. Positively regulated by both LEAFY and APETALA1. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2. Up-regulated by HUA2. {ECO:0000269\|PubMed:10198637, ECO:0000269\|PubMed:11058164, ECO:0000269\|PubMed:1675158, ECO:0000269\|PubMed:17794879, ECO:0000269\|PubMed:18281509, ECO:0000269\|PubMed:19783648, ECO:0000269\|PubMed:9783581}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	EU551772	0.0	EU551772.1 Capsella bursa-pastoris AGAMOUS-like protein (AGa) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_010445284.1	0.0	PREDICTED: floral homeotic protein AGAMOUS
Swissprot	P17839	0.0	AG_ARATH; Floral homeotic protein AGAMOUS
TrEMBL	D7M9D0	0.0	D7M9D0_ARALL; Uncharacterized protein
STRING	Cagra.9536s0004.1.p	0.0	(Capsella grandiflora)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM6181	22	37

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G18960.1	1e-164	MIKC_MADS family protein

Link Out ? help Back to Top
Phytozome	Cagra.9536s0004.1.p

Publications ? help Back to Top
Huang Z, et al. APETALA2 antagonizes the transcriptional activity of AGAMOUS in regulating floral stem cells in Arabidopsis thaliana. New Phytol., 2017. 215(3): p. 1197-1209 [PMID:27604611] Rong XF, et al. Type-B ARRs Control Carpel Regeneration Through Mediating AGAMOUS Expression in Arabidopsis. Plant Cell Physiol., 2018. 59(4): p. 756-764 [PMID:29186581] Uemura A, et al. Regulation of floral meristem activity through the interaction of AGAMOUS, SUPERMAN, and CLAVATA3 in Arabidopsis. Plant Reprod, 2018. 31(1): p. 89-105 [PMID:29218596]