PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Brachypodieae; Brachypodium

Family

GRAS

Protein Properties

Length: 494aa MW: 51888.7 Da PI: 5.0397

Description

GRAS family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Brast01G200600.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	GRAS	422.5	3.6e-129	84	453	1	373
GRAS 1 lvelLlecAeavssgdlelaqalLarlsel...aspdgdpmqRlaayfteALaarlarsvselykalppsetseknsseelaalklfsevs 88 lv+lL++cA av++gd+++a alLa + a ++++ + R+a +ft AL++rl+ ++ +p++ + ++++ ++ f+e++ Brast01G200600.1.p 84 LVHLLMSCAGAVEAGDHAAAAALLADANASlaaAVSTASGIGRVAVHFTDALSRRLFLPSPAAG--TAPTPPPAAADPDHAFLYHHFYEAC 172 68**********************99987756666799*************9555555..44444444568999999**** PP GRAS 89 PilkfshltaNqaIleavegeervHiiDfdisqGlQWpaLlqaLasRpegppslRiTgvgspesgskeeleetgerLakfAeelgvpfefn 179 P+lkf+h+taNqaIlea++g++ vH++Df+++qGlQWpaL+qaLa Rp+gpp lRiTg+g+p++ ++el+ +g rLa++A++++v+f+f+ Brast01G200600.1.p 173 PYLKFAHFTANQAILEAFHGCDSVHVVDFSLMQGLQWPALIQALALRPGGPPFLRITGIGPPSPPGRDELRDVGLRLAELARSVRVRFSFR 263 *************************************************************************************** PP GRAS 180 vlvakrledleleeLrvkpgEalaVnlvlqlhrlldesvsleserdevLklvkslsPkvvvvveqeadhnsesFlerflealeyysalfds 270 ++a++l+++++++L++ pgEa+aVn+vlqlhrll+ +++ + ++d+vL +v sl+Pk+++vveqeadhn++ Fl+rf+eal yysa+fds Brast01G200600.1.p 264 GVAANTLDEVRPWMLQTAPGEAVAVNSVLQLHRLLAGPADRQAPIDAVLGCVASLQPKIFTVVEQEADHNKPGFLDRFTEALFYYSAVFDS 354 ***************************************************************************************** PP GRAS 271 leaklpreseerikvErellgreivnvvacegaerrerhetlekWrerleeaGFkpvplsekaakqaklllrkvksdgyrveeesgslvlg 361 l+ +++ + + +++l+rei+++v++ega+r erhe l++Wr+rl++aG+++vpl+ a qa++l+ ++++g++vee +g+l+lg Brast01G200600.1.p 355 LDDA----TSASNAMAEAYLQREICDIVCHEGAARMERHEPLSRWRDRLGRAGLRAVPLGPGALRQARMLVGLFSGEGHSVEEAEGCLTLG 441 777....36667777778********************************************************************* PP GRAS 362 WkdrpLvsvSaW 373 W++r+L+s+SaW Brast01G200600.1.p 442 WHGRTLFSASAW 453 ********** PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50985	63.321	58	434	IPR005202	Transcription factor GRAS
Pfam	PF03514	1.2E-126	84	453	IPR005202	Transcription factor GRAS

Sequence ? help Back to Top
Protein Sequence Length: 494 aa Download sequence Send to blast
MAMGTFPFQW PVDPAPLSSG VDGSLLPSFL PPPQPAAAPD DGAAYYAASD MHGAPPSLPE 60 LAAPLAAAEL AMRRAEEEVA GIRLVHLLMS CAGAVEAGDH AAAAALLADA NASLAAAVST 120 ASGIGRVAVH FTDALSRRLF LPSPAAGTAP TPPPAAADPD HAFLYHHFYE ACPYLKFAHF 180 TANQAILEAF HGCDSVHVVD FSLMQGLQWP ALIQALALRP GGPPFLRITG IGPPSPPGRD 240 ELRDVGLRLA ELARSVRVRF SFRGVAANTL DEVRPWMLQT APGEAVAVNS VLQLHRLLAG 300 PADRQAPIDA VLGCVASLQP KIFTVVEQEA DHNKPGFLDR FTEALFYYSA VFDSLDDATS 360 ASNAMAEAYL QREICDIVCH EGAARMERHE PLSRWRDRLG RAGLRAVPLG PGALRQARML 420 VGLFSGEGHS VEEAEGCLTL GWHGRTLFSA SAWGAAGDGE ENIDSNNSNI GASSGSDSNN 480 SSCGVVGATN MFL*

Sequence ? help Back to Top

Protein Sequence Length: 494 aa Download sequence Send to blast

MAMGTFPFQW PVDPAPLSSG VDGSLLPSFL PPPQPAAAPD DGAAYYAASD MHGAPPSLPE  60
LAAPLAAAEL AMRRAEEEVA GIRLVHLLMS CAGAVEAGDH AAAAALLADA NASLAAAVST  120
ASGIGRVAVH FTDALSRRLF LPSPAAGTAP TPPPAAADPD HAFLYHHFYE ACPYLKFAHF  180
TANQAILEAF HGCDSVHVVD FSLMQGLQWP ALIQALALRP GGPPFLRITG IGPPSPPGRD  240
ELRDVGLRLA ELARSVRVRF SFRGVAANTL DEVRPWMLQT APGEAVAVNS VLQLHRLLAG  300
PADRQAPIDA VLGCVASLQP KIFTVVEQEA DHNKPGFLDR FTEALFYYSA VFDSLDDATS  360
ASNAMAEAYL QREICDIVCH EGAARMERHE PLSRWRDRLG RAGLRAVPLG PGALRQARML  420
VGLFSGEGHS VEEAEGCLTL GWHGRTLFSA SAWGAAGDGE ENIDSNNSNI GASSGSDSNN  480
SSCGVVGATN MFL*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5b3g_A	1e-55	72	453	7	378	Protein SCARECROW
5b3h_A	1e-55	72	453	6	377	Protein SCARECROW
5b3h_D	1e-55	72	453	6	377	Protein SCARECROW
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Transcription coactivator of the zinc finger transcription factors GAF1/IDD2 and ENY/IDD1 in regulation of gibberellin homeostasis and signaling (PubMed:25035403). No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression. In contrast to RGA, it is less sensitive to GA. Its activity is probably regulated by other phytohormones such as auxin and ethylene. {ECO:0000269\|PubMed:11487693, ECO:0000269\|PubMed:11606551, ECO:0000269\|PubMed:11606552, ECO:0000269\|PubMed:14973286, ECO:0000269\|PubMed:15128937, ECO:0000269\|PubMed:16034591, ECO:0000269\|PubMed:17933900, ECO:0000269\|PubMed:25035403, ECO:0000269\|PubMed:9389651}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Brast01G200600.1.p

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	HG670306	0.0	HG670306.1 Triticum aestivum chromosome 3B, genomic scaffold, cultivar Chinese Spring.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_003566939.1	0.0	DELLA protein SLR1
Swissprot	Q9LQT8	1e-128	GAI_ARATH; DELLA protein GAI
TrEMBL	I1HPW5	0.0	I1HPW5_BRADI; Uncharacterized protein
STRING	BRADI2G45117.1	0.0	(Brachypodium distachyon)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP998	38	138

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G14920.1	1e-114	GRAS family protein

Link Out ? help Back to Top
Phytozome	Brast01G200600.1.p

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Gallego-Giraldo C, et al. Role of the gibberellin receptors GID1 during fruit-set in Arabidopsis. Plant J., 2014. 79(6): p. 1020-1032 [PMID:24961590] Fukazawa J, et al. DELLAs function as coactivators of GAI-ASSOCIATED FACTOR1 in regulation of gibberellin homeostasis and signaling in Arabidopsis. Plant Cell, 2014. 26(7): p. 2920-38 [PMID:25035403] Marín-de la Rosa N, et al. Genome Wide Binding Site Analysis Reveals Transcriptional Coactivation of Cytokinin-Responsive Genes by DELLA Proteins. PLoS Genet., 2015. 11(7): p. e1005337 [PMID:26134422] Shi H,Wei Y,Wang Q,Reiter RJ,He C Melatonin mediates the stabilization of DELLA proteins to repress the floral transition in Arabidopsis. J. Pineal Res., 2016. 60(3): p. 373-9 [PMID:26887824] Lee SA, et al. Interplay between ABA and GA Modulates the Timing of Asymmetric Cell Divisions in the Arabidopsis Root Ground Tissue. Mol Plant, 2016. 9(6): p. 870-84 [PMID:26970019] Qu J,Kang SG,Hah C,Jang JC Molecular and cellular characterization of GA-Stimulated Transcripts GASA4 and GASA6 in Arabidopsis thaliana. Plant Sci., 2016. 246: p. 1-10 [PMID:26993231] Shahnejat-Bushehri S,Nobmann B,Devi Allu A,Balazadeh S JUB1 suppresses Pseudomonas syringae-induced defense responses through accumulation of DELLA proteins. Plant Signal Behav, 2016. 11(6): p. e1181245 [PMID:27159137] Shahnejat-Bushehri S,Tarkowska D,Sakuraba Y,Balazadeh S Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. Nat Plants, 2016. 2: p. 16013 [PMID:27249348] Wang H, et al. The DELLA-CONSTANS Transcription Factor Cascade Integrates Gibberellic Acid and Photoperiod Signaling to Regulate Flowering. Plant Physiol., 2016. 172(1): p. 479-88 [PMID:27406167] Li W,Wang H,Yu D Arabidopsis WRKY Transcription Factors WRKY12 and WRKY13 Oppositely Regulate Flowering under Short-Day Conditions. Mol Plant, 2016. 9(11): p. 1492-1503 [PMID:27592586] Liu B,De Storme N,Geelen D Gibberellin Induces Diploid Pollen Formation by Interfering with Meiotic Cytokinesis. Plant Physiol., 2017. 173(1): p. 338-353 [PMID:27621423] Matsuoka K, et al. Differential Cellular Control by Cotyledon-Derived Phytohormones Involved in Graft Reunion of Arabidopsis Hypocotyls. Plant Cell Physiol., 2016. 57(12): p. 2620-2631 [PMID:27986917] Zentella R, et al. The Arabidopsis O-fucosyltransferase SPINDLY activates nuclear growth repressor DELLA. Nat. Chem. Biol., 2017. 13(5): p. 479-485 [PMID:28244988] Zhang Y, et al. GA-DELLA pathway is involved in regulation of nitrogen deficiency-induced anthocyanin accumulation. Plant Cell Rep., 2017. 36(4): p. 557-569 [PMID:28275852] Zhang L,Chen L,Yu D Transcription Factor WRKY75 Interacts with DELLA Proteins to Affect Flowering. Plant Physiol., 2018. 176(1): p. 790-803 [PMID:29133369] Nelson SK,Ariizumi T,Steber CM Biology in the Dry Seed: Transcriptome Changes Associated with Dry Seed Dormancy and Dormancy Loss in the Arabidopsis GA-Insensitive sleepy1-2 Mutant. Front Plant Sci, 2017. 8: p. 2158 [PMID:29312402] Liu B,De Storme N,Geelen D Cold-Induced Male Meiotic Restitution in Arabidopsis thaliana Is Not Mediated by GA-DELLA Signaling. Front Plant Sci, 2018. 9: p. 91 [PMID:29459879] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486] Felipo-Benavent A, et al. Regulation of xylem fiber differentiation by gibberellins through DELLA-KNAT1 interaction. Development, 2019. [PMID:30389856] Wright DA, et al. Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation. Nucleic Acids Res., 1997. 25(13): p. 2679-80 [PMID:9185581]