PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Boechereae; Boechera

Family

LBD

Protein Properties

Length: 202aa MW: 21947.8 Da PI: 7.5609

Description

LBD family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Bostr.26959s0351.2.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	DUF260	145.2	1.8e-45	9	108	1	100
DUF260 1 aCaaCkvlrrkCakdCvlapyfpaeqpkkfanvhklFGasnvlkllkalpeeeredamsslvyeAearardPvyGavgvilklqqqleq 89 +CaaCk+lrrkC+++Cv+apyfp +qp+kfanvhk+FGasnv+kll++l++++reda++sl+yeA++r+rdPvyG+vgvi+ lq+ql+q Bostr.26959s0351.2.p 9 PCAACKFLRRKCQPECVFAPYFPPDQPQKFANVHKVFGASNVTKLLNELHPSQREDAVNSLAYEADMRLRDPVYGCVGVISLLQHQLRQ 97 7************************************************************************************** PP DUF260 90 lkaelallkee 100 l+ +l+ +k+e Bostr.26959s0351.2.p 98 LQIDLSCAKSE 108 ***999887 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50891	27.201	8	109	IPR004883	Lateral organ boundaries, LOB
Pfam	PF03195	1.2E-44	9	106	IPR004883	Lateral organ boundaries, LOB

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009799	Biological Process	specification of symmetry
GO:0009944	Biological Process	polarity specification of adaxial/abaxial axis
GO:0009954	Biological Process	proximal/distal pattern formation
GO:0048441	Biological Process	petal development
GO:0005654	Cellular Component	nucleoplasm

Sequence ? help Back to Top
Protein Sequence Length: 202 aa Download sequence Send to blast
MASSSTNSPC AACKFLRRKC QPECVFAPYF PPDQPQKFAN VHKVFGASNV TKLLNELHPS 60 QREDAVNSLA YEADMRLRDP VYGCVGVISL LQHQLRQLQI DLSCAKSELS KYQSLGILAA 120 THQSLGINLL AGAADGTATA TVRDHHYHHH QFFPREQMFG GLDVPAGNNY DGGILAIGQI 180 TQFQQPRAAA GDDGRRTVDP S*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5ly0_A	2e-54	3	112	5	114	LOB family transfactor Ramosa2.1
5ly0_B	2e-54	3	112	5	114	LOB family transfactor Ramosa2.1
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Functional Description ? help Back to Top
Source	Description
UniProt	Negative regulator of cell proliferation in the adaxial side of leaves. Regulates the formation of a symmetric lamina and the establishment of venation. Positively regulates LATERAL ORGAN BOUNDARIES (LOB) within the shoot apex, and the class III HD-ZIP genes REV, PHB, and PHV. Interacts directly with ASYMMETRIC LEAVES 1 (AS1) to repress the knox homeobox genes KNAT1, KNAT2, and KNAT6 and the abaxial determinants ARF3, KAN2 and YAB5. May act in parallel with the RDR6-SGS3-AGO7 pathway, an endogenous RNA silencing pathway, to regulate the leaf morphogenesis (PubMed:11311158, PubMed:12787254, PubMed:12874130, PubMed:14508003, PubMed:16006579, PubMed:16699177, PubMed:17395603, PubMed:17559509). Required for the binding of AS1 to the KNOX genes (PubMed:23271976). Involved in leaf polarity establishment by functioning cooperatively with RH10 or RID2 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures (PubMed:27334696). {ECO:0000269\|PubMed:11311158, ECO:0000269\|PubMed:12787254, ECO:0000269\|PubMed:12874130, ECO:0000269\|PubMed:14508003, ECO:0000269\|PubMed:16006579, ECO:0000269\|PubMed:16699177, ECO:0000269\|PubMed:17395603, ECO:0000269\|PubMed:17559509, ECO:0000269\|PubMed:23271976, ECO:0000269\|PubMed:27334696}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00211	DAP	Transfer from AT1G65620	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Bostr.26959s0351.2.p

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Negatively regulated by SHOOT MERISTEMLESS (STM). {ECO:0000269\|PubMed:11934861}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB080802	0.0	AB080802.1 Arabidopsis thaliana AS2 mRNA for ASYMMETRIC LEAVES2, complete cds.
GenBank	AB473833	0.0	AB473833.1 Arabidopsis thaliana AS2 mRNA for ASYMMETRIC LEAVES2, complete cds.
GenBank	AF447887	0.0	AF447887.1 Arabidopsis thaliana LOB DOMAIN 6 (LBD6) mRNA, complete cds.
GenBank	AY519483	0.0	AY519483.1 Arabidopsis thaliana ecotype Blanes AS2 (At1G65620) gene, complete cds.
GenBank	BT025163	0.0	BT025163.1 Arabidopsis thaliana At1g65620 mRNA, complete cds.
GenBank	CP002684	0.0	CP002684.1 Arabidopsis thaliana chromosome 1 sequence.
GenBank	F5I14	0.0	AC001229.1 Sequence of BAC F5I14 from Arabidopsis thaliana chromosome 1, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020889693.1	1e-149	LOB domain-containing protein 6
Refseq	XP_020889694.1	1e-149	LOB domain-containing protein 6
Refseq	XP_020889696.1	1e-149	LOB domain-containing protein 6
Refseq	XP_020889697.1	1e-149	LOB domain-containing protein 6
Swissprot	O04479	1e-127	AS2_ARATH; Protein ASYMMETRIC LEAVES 2
TrEMBL	D7KSE5	1e-147	D7KSE5_ARALL; Uncharacterized protein
STRING	Bostr.26959s0351.1.p	1e-149	(Boechera stricta)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G65620.4	1e-129	LBD family protein

Link Out ? help Back to Top
Phytozome	Bostr.26959s0351.2.p

Publications ? help Back to Top
Machida C,Nakagawa A,Kojima S,Takahashi H,Machida Y The complex of ASYMMETRIC LEAVES (AS) proteins plays a central role in antagonistic interactions of genes for leaf polarity specification in Arabidopsis. Wiley Interdiscip Rev Dev Biol, 2015 Nov-Dec. 4(6): p. 655-71 [PMID:26108442] Mateo-Bonmatí E, et al. Plastid control of abaxial-adaxial patterning. Sci Rep, 2015. 5: p. 15975 [PMID:26522839] Li Z, et al. Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis. J Integr Plant Biol, 2016. 58(12): p. 959-970 [PMID:27273574] Matsumura Y, et al. A genetic link between epigenetic repressor AS1-AS2 and a putative small subunit processome in leaf polarity establishment of Arabidopsis. Biol Open, 2016. 5(7): p. 942-54 [PMID:27334696] Wang Z,Wang Y,Kohalmi SE,Amyot L,Hannoufa A SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 2 controls floral organ development and plant fertility by activating ASYMMETRIC LEAVES 2 in Arabidopsis thaliana. Plant Mol. Biol., 2016. 92(6): p. 661-674 [PMID:27605094] Vial-Pradel S, et al. Arabidopsis Zinc-Finger-Like Protein ASYMMETRIC LEAVES2 (AS2) and Two Nucleolar Proteins Maintain Gene Body DNA Methylation in the Leaf Polarity Gene ETTIN (ARF3). Plant Cell Physiol., 2018. 59(7): p. 1385-1397 [PMID:29415182] Silverblatt-Buser EW,Frick MA,Rabeler C,Kaplinsky NJ Genetic Interactions Between BOB1 and Multiple 26S Proteasome Subunits Suggest a Role for Proteostasis in Regulating Arabidopsis Development. G3 (Bethesda), 2018. 8(4): p. 1379-1390 [PMID:29487187]