PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Bostr.19424s0222.2.p
Organism
Boechera stricta
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Boechereae; Boechera
Family bHLH
Protein Properties Length: 392aa    MW: 43948.2 Da    PI: 5.4876
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Bostr.19424s0222.2.pgenomeJGIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH38.12.7e-12172223155
                           CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
                   HLH   1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                           +r++h ++Er+RR+++N+ +  Lr+l+P +   + ++ + a+i   A+e++++L+
  Bostr.19424s0222.2.p 172 QRMTHIAVERNRRKQMNEHLRVLRSLMPGS---YVQRGDQASIIGGAIEFVRELE 223
                           79****************************...9*****************9995 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:4.10.280.101.3E-13170226IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.66E-12170227No hitNo description
SuperFamilySSF474592.22E-16171236IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS5088815.428171222IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.2E-9172223IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003533.4E-9177228IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd048730.00264305369No hitNo description
PROSITE profilePS516718.596305380IPR002912ACT domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0010377Biological Processguard cell fate commitment
GO:0045597Biological Processpositive regulation of cell differentiation
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0051782Biological Processnegative regulation of cell division
GO:0005634Cellular Componentnucleus
GO:0016597Molecular Functionamino acid binding
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 392 aa     Download sequence    Send to blast
MIDYMFNRNL QQQQKQSMPQ QQQHQLSPTG FGATSSFDKM NFADVMQFAD FSPKLALNQT  60
RNQDDQESGI DPVYFLKFPV LNDKIEDHNQ TQHLMPSHQT SQEGGECGGN IGNVFLEEKE  120
DQDDDNDNNS VQLRFIGGEE EDRENKNVTT KEVKSKRKRA RTSKTSEEVE SQRMTHIAVE  180
RNRRKQMNEH LRVLRSLMPG SYVQRGDQAS IIGGAIEFVR ELEQLLQCLE SQKRRRILGE  240
TGRDMTTTTT SSSSPITAVA NQAQPLIITG NVTELEGGGG LREETAENKS CLADVEVKLL  300
GFDAMIKILS RRRPGQLIKT IAALEDLHLS ILHTNITTME QTVLYSFNVK ITSETRFTAE  360
DIASSIQQIF SFIHANTTIS GSSNLGNVVF A*
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1172183RMTHIAVERNRR
2232236KRRRI
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269|PubMed:17088607, ECO:0000269|PubMed:17183265, ECO:0000269|PubMed:17183267, ECO:0000269|PubMed:24571519, ECO:0000269|PubMed:24654956}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapBostr.19424s0222.2.p
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269|PubMed:22442411}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK2213240.0AK221324.1 Arabidopsis thaliana mRNA for putative bHLH transcription factor, complete cds, clone: RAFL25-14-O10.
GenBankBT0289610.0BT028961.1 Arabidopsis thaliana At3g24140 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_002883481.20.0transcription factor FAMA
SwissprotQ56YJ80.0FAMA_ARATH; Transcription factor FAMA
TrEMBLD7L4810.0D7L481_ARALL; Uncharacterized protein
STRINGBostr.19424s0222.1.p0.0(Boechera stricta)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G24140.10.0bHLH family protein
Publications ? help Back to Top
  1. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  2. Matos JL, et al.
    Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module.
    Elife, 2015.
    [PMID:25303364]
  3. Chen L, et al.
    NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis.
    Development, 2016. 143(9): p. 1600-11
    [PMID:26989174]
  4. Lee JH,Jung JH,Park CM
    Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis.
    Plant Cell, 2017. 29(11): p. 2817-2830
    [PMID:29070509]
  5. Han X, et al.
    Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons.
    Plant Physiol., 2018. 176(4): p. 2871-2885
    [PMID:29496884]
  6. Li X,Yang R,Chen H
    The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA.
    PLoS ONE, 2018. 13(3): p. e0193458
    [PMID:29513733]