PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa

Family

WRKY

Protein Properties

Length: 330aa MW: 34785.4 Da PI: 10.33

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
BGIOSGA009380-PA	genome	RIS	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	97.7	7.4e-31	251	309	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 D ++WrKYGqK++kgs+fpr+YY+C++ gCp++k+ver+ dp+++++tYegeH+h+ BGIOSGA009380-PA 251 ADDFSWRKYGQKPIKGSPFPRGYYKCSTLrGCPARKHVERDPADPSMLIVTYEGEHRHT 309 699*********************9988**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF10533	1.7E-20	199	248	IPR018872	Zn-cluster domain
Gene3D	G3DSA:2.20.25.80	5.9E-32	238	309	IPR003657	WRKY domain
PROSITE profile	PS50811	29.585	245	311	IPR003657	WRKY domain
SuperFamily	SSF118290	1.18E-25	248	309	IPR003657	WRKY domain
SMART	SM00774	5.4E-37	250	310	IPR003657	WRKY domain
Pfam	PF03106	6.9E-26	252	308	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009737	Biological Process	response to abscisic acid
GO:0009938	Biological Process	negative regulation of gibberellic acid mediated signaling pathway
GO:0010200	Biological Process	response to chitin
GO:0042742	Biological Process	defense response to bacterium
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005516	Molecular Function	calmodulin binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:1990841	Molecular Function	promoter-specific chromatin binding

Sequence ? help Back to Top
Protein Sequence Length: 330 aa Download sequence Send to blast
MITMDLMSGY GRVDEQVAIQ EAAAAGLRGM EHLILQLSQT GTSERSPAPA PAQEQQQQQQ 60 VDCREITDMT VSKFKKVISM LNRTGHARFR RGPVVAQSSG PAASEPAPVR SSPSAVSRPM 120 TLDFTKAASG YGKDAGFSVS GISAASSSFL SSVTGDGSVS NGRGGGSSSL MLPPPPATSC 180 GKPPLSSAAA AMSAGVGHKR KCHDHAHSEN IAGGKYGSTG GRCHCSKRRK HRVKRTIRVP 240 AISSKVADIP ADDFSWRKYG QKPIKGSPFP RGYYKCSTLR GCPARKHVER DPADPSMLIV 300 TYEGEHRHTP SAAGQDHPPA PPPPLALPLA

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	2e-20	238	308	2	71	WRKY transcription factor 1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Os.49656	0.0	callus\| flower\| leaf\| panicle\| root

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Highly expressed in aleurone cells. In seeds, predominantly present in the plumule, radicle and scutellum of the embryo. {ECO:0000250\|UniProtKB:Q0JEE2}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts, when in complex with WRKY71, specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Represses specifically gibberellic acid (GA)-induced promoters in aleurone cells, probably by interfering with GAM1. {ECO:0000250\|UniProtKB:Q0JEE2}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00464	DAP	Transfer from AT4G31550	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by abscisic acid (ABA) in aleurone cells (By similarity) (PubMed:15618416). Accumulates in response to uniconazole, a gibberellic acid (GA) biosynthesis inhibitor (By similarity). Repressed by GA (PubMed:15618416). {ECO:0000250\|UniProtKB:Q0JEE2, ECO:0000269\|PubMed:15618416}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK100954	0.0	AK100954.1 Oryza sativa Japonica Group cDNA clone:J023139N16, full insert sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_015633572.1	0.0	WRKY transcription factor WRKY51
Swissprot	Q6IEN1	0.0	WRK51_ORYSI; WRKY transcription factor WRKY51
TrEMBL	A0A0E0GYL2	0.0	A0A0E0GYL2_ORYNI; Uncharacterized protein
STRING	ONIVA04G04750.1	0.0	(Oryza nivara)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP617	38	171

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G31550.1	1e-88	WRKY DNA-binding protein 11

Publications ? help Back to Top
Zhang ZL, et al. A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells. Plant Physiol., 2004. 134(4): p. 1500-13 [PMID:15047897] Xie Z, et al. Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells. Plant Physiol., 2005. 137(1): p. 176-89 [PMID:15618416]