PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa

Family

WRKY

Protein Properties

Length: 253aa MW: 27081.1 Da PI: 10.0636

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
BGIOSGA008165-PA	genome	RIS	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	93.4	1.7e-29	168	225	2	58
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnh 58 D y+WrKYGqK++kgs++pr+YY+C++ gCp++k+ver+ +p ++++tY g+H+h BGIOSGA008165-PA 168 ADDYSWRKYGQKPIKGSPYPRGYYKCSTVrGCPARKHVERDPGEPAMLIVTYDGDHRH 225 69**********************9888*************************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF10533	8.5E-14	125	165	IPR018872	Zn-cluster domain
Gene3D	G3DSA:2.20.25.80	1.7E-31	155	228	IPR003657	WRKY domain
PROSITE profile	PS50811	30.515	162	228	IPR003657	WRKY domain
SuperFamily	SSF118290	1.7E-25	163	228	IPR003657	WRKY domain
SMART	SM00774	2.5E-33	167	227	IPR003657	WRKY domain
Pfam	PF03106	1.1E-24	169	225	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 253 aa Download sequence Send to blast
MADPFPAAAR GGEQGGGTAG QLVATPSRLR TAVASMLNRT GHARFRRAAP VVVQEEEDEA 60 AAAARDAVVR CDGLSASASS SFPSSVTGVT GDGSVSNARA VLPAAGAGDK PPPMQSASDY 120 ASDGRLKRSS DDDGERCHCS KKKRKASWRA RRRIRVPAIS SRNADIPADD YSWRKYGQKP 180 IKGSPYPRGY YKCSTVRGCP ARKHVERDPG EPAMLIVTYD GDHRHGEPGH RRPDEAATTT 240 EHRTTDQTTG RLL

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	7e-20	155	228	2	74	WRKY transcription factor 1
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Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	32995796	0.0

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Highly expressed in aleurone cells. In seeds, predominantly present in the plumule, radicle and scutellum of the embryo. {ECO:0000269\|PubMed:16623886}.
Uniprot	TISSUE SPECIFICITY: In young, mature and senescent leaves. {ECO:0000269\|PubMed:11722756}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000, probably by activating the jasmonic acid (JA)- signaling pathway (PubMed:26433201). {ECO:0000250, ECO:0000269\|PubMed:26433201}.
UniProt	Transcription factor. Interacts, when in complex with WRKY71, specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Represses specifically gibberellic acid (GA)-induced promoters in aleurone cells, probably by interfering with GAM1. {ECO:0000269\|PubMed:16623886}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by abscisic acid (ABA) in aleurone cells (PubMed:15618416, PubMed:16623886). Accumulates in response to uniconazole, a gibberellic acid (GA) biosynthesis inhibitor (PubMed:16623886). Repressed by GA (PubMed:15618416). {ECO:0000269\|PubMed:15618416, ECO:0000269\|PubMed:16623886}.
UniProt	INDUCTION: Strongly during leaf senescence (PubMed:11722756). Repressed by rhizobacterium B.cereus AR156 in leaves, and to a lower extent, by P.fluorescens WCS417r (PubMed:26433201). {ECO:0000269\|PubMed:11722756, ECO:0000269\|PubMed:26433201}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY676923	0.0	AY676923.1 Oryza sativa (indica cultivar-group) transcription factor WRKY42 (WRKY42) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_015624842.1	0.0	WRKY transcription factor WRKY51-like
Swissprot	Q0JEE2	4e-51	WRK51_ORYSJ; WRKY transcription factor WRKY51
Swissprot	Q9SV15	4e-51	WRK11_ARATH; Probable WRKY transcription factor 11
TrEMBL	A0A0D9YRZ6	0.0	A0A0D9YRZ6_9ORYZ; Uncharacterized protein
TrEMBL	A0A0E0IBA4	0.0	A0A0E0IBA4_ORYNI; Uncharacterized protein
TrEMBL	I1P078	0.0	I1P078_ORYGL; Uncharacterized protein
TrEMBL	Q6IEN9	0.0	Q6IEN9_ORYSI; Transcription factor WRKY42
STRING	OGLUM02G16050.1	0.0	(Oryza glumipatula)
STRING	ONIVA08G14000.1	0.0	(Oryza nivara)
STRING	ORGLA02G0138400.1	0.0	(Oryza glaberrima)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP617	38	171

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G31550.1	1e-36	WRKY DNA-binding protein 11

Publications ? help Back to Top
Haas BJ, et al. Full-length messenger RNA sequences greatly improve genome annotation. Genome Biol., 2002. 3(6): p. RESEARCH0029 [PMID:12093376] Kikuchi S, et al. Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice. Science, 2003. 301(5631): p. 376-9 [PMID:12869764] Zhang ZL, et al. A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells. Plant Physiol., 2004. 134(4): p. 1500-13 [PMID:15047897] Zhang Y,Wang L The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol. Biol., 2005. 5: p. 1 [PMID:15629062] Wu KL,Guo ZJ,Wang HH,Li J The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res., 2005. 12(1): p. 9-26 [PMID:16106749] Xie Z, et al. Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells. Plant J., 2006. 46(2): p. 231-42 [PMID:16623886] Liu X,Bai X,Wang X,Chu C OsWRKY71, a rice transcription factor, is involved in rice defense response. J. Plant Physiol., 2007. 164(8): p. 969-79 [PMID:16919842] Yilmaz A, et al. GRASSIUS: a platform for comparative regulatory genomics across the grasses. Plant Physiol., 2009. 149(1): p. 171-80 [PMID:18987217] Zhang ZL, et al. A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells. Plant Mol. Biol., 2009. 70(1-2): p. 139-51 [PMID:19199048] Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro. Plant Methods, 2010. 6: p. 25 [PMID:21108821] Brand LH, et al. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA. PLoS ONE, 2013. 8(10): p. e75177 [PMID:24146751] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Jiang CH, et al. Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. J. Exp. Bot., 2016. 67(1): p. 157-74 [PMID:26433201] Hwang SH, et al. OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae. Plant Cell Rep., 2016. 35(9): p. 1975-85 [PMID:27300023] Ali MA, et al. Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis. J. Plant Physiol., 2018. 226: p. 12-21 [PMID:29689430]