PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; stem eudicotyledons; Ranunculales; Ranunculaceae; Thalictroideae; Aquilegia

Family

C2H2

Protein Properties

Length: 83aa MW: 9594.03 Da PI: 10.2802

Description

C2H2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Aqcoe3G254800.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	zf-C2H2	12.7	0.00039	22	45	2	23
EET..TTTEEESSHHHHHHHHHHT CS zf-C2H2 2 kCp..dCgksFsrksnLkrHirtH 23 kCp C++sF+ + H+r+H Aqcoe3G254800.1.p 22 KCPwkGCTMSFKWAWAKTEHLRKH 45 89*********************9 PP
2	zf-C2H2	15.8	3.9e-05	51	77	1	23
EEET..TTTEEESSHHHHHHHHHH..T CS zf-C2H2 1 ykCp..dCgksFsrksnLkrHirt..H 23 y+C+ Cg +F+ s++ rH r+ H Aqcoe3G254800.1.p 51 YQCKikVCGLTFRYVSDFSRHKRRngH 77 9**********************9776 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00355	0.0024	21	45	IPR015880	Zinc finger, C2H2-like
PROSITE profile	PS50157	9.764	21	50	IPR007087	Zinc finger, C2H2
Gene3D	G3DSA:3.30.160.60	1.9E-7	22	45	IPR013087	Zinc finger C2H2-type/integrase DNA-binding domain
PROSITE pattern	PS00028	0	23	45	IPR007087	Zinc finger, C2H2
SuperFamily	SSF57667	1.6E-10	31	73	No hit	No description
Gene3D	G3DSA:3.30.160.60	2.0E-8	46	73	IPR013087	Zinc finger C2H2-type/integrase DNA-binding domain
SMART	SM00355	3.4	51	77	IPR015880	Zinc finger, C2H2-like
PROSITE profile	PS50157	10.949	51	82	IPR007087	Zinc finger, C2H2
PROSITE pattern	PS00028	0	53	77	IPR007087	Zinc finger, C2H2

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0003676	Molecular Function	nucleic acid binding
GO:0046872	Molecular Function	metal ion binding

Sequence ? help Back to Top
Protein Sequence Length: 83 aa Download sequence Send to blast
MHPQGMREAV QVTQVCDSSS LKCPWKGCTM SFKWAWAKTE HLRKHTGERS YQCKIKVCGL 60 TFRYVSDFSR HKRRNGHHVD SP*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6a57_A	6e-26	17	79	72	134	Lysine-specific demethylase REF6
6a58_A	6e-26	17	79	72	134	Lysine-specific demethylase REF6
6a59_A	6e-26	17	79	72	134	Lysine-specific demethylase REF6
6jnl_A	3e-26	17	79	33	95	Lysine-specific demethylase REF6
6jnm_A	3e-26	17	79	33	95	Lysine-specific demethylase REF6
6jnm_B	3e-26	17	79	33	95	Lysine-specific demethylase REF6
6jnn_A	3e-26	17	79	33	95	Lysine-specific demethylase REF6
6jnn_B	3e-26	17	79	33	95	Lysine-specific demethylase REF6
6jnn_G	3e-26	17	79	33	95	Lysine-specific demethylase REF6
6jnn_N	3e-26	17	79	33	95	Lysine-specific demethylase REF6
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Histone demethylase that demethylates 'Lys-27' (H3K27me) of histone H3. Demethylates both tri- (H3K27me3) and di-methylated (H3K27me2) H3K27me (PubMed:21642989, PubMed:27111035). Demethylates also H3K4me3/2 and H3K36me3/2 in an in vitro assay (PubMed:20711170). Involved in the transcriptional regulation of hundreds of genes regulating developmental patterning and responses to various stimuli (PubMed:18467490). Binds DNA via its four zinc fingers in a sequence-specific manner, 5'-CTCTGYTY-3', to promote the demethylation of H3K27me3 and the regulation of organ boundary formation (PubMed:27111034, PubMed:27111035). Involved in the regulation of flowering time by repressing FLOWERING LOCUS C (FLC) expression (PubMed:15377760). Interacts with the NF-Y complexe to regulate SOC1 (PubMed:25105952). Mediates the recruitment of BRM to its target loci (PubMed:27111034). {ECO:0000269\|PubMed:15377760, ECO:0000269\|PubMed:18467490, ECO:0000269\|PubMed:20711170, ECO:0000269\|PubMed:21642989, ECO:0000269\|PubMed:25105952, ECO:0000269\|PubMed:27111034, ECO:0000269\|PubMed:27111035}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_028071343.1	5e-31	probable lysine-specific demethylase ELF6
Swissprot	Q9STM3	6e-25	REF6_ARATH; Lysine-specific demethylase REF6
TrEMBL	A0A2G5D1B2	2e-34	A0A2G5D1B2_AQUCA; Uncharacterized protein
STRING	Aquca_030_00119.1	4e-35	(Aquilegia coerulea)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G48430.1	2e-27	relative of early flowering 6

Link Out ? help Back to Top
Phytozome	Aqcoe3G254800.1.p

Publications ? help Back to Top
Li C, et al. Concerted genomic targeting of H3K27 demethylase REF6 and chromatin-remodeling ATPase BRM in Arabidopsis. Nat. Genet., 2016. 48(6): p. 687-93 [PMID:27111034] Cui X, et al. REF6 recognizes a specific DNA sequence to demethylate H3K27me3 and regulate organ boundary formation in Arabidopsis. Nat. Genet., 2016. 48(6): p. 694-9 [PMID:27111035] Hyun Y, et al. Multi-layered Regulation of SPL15 and Cooperation with SOC1 Integrate Endogenous Flowering Pathways at the Arabidopsis Shoot Meristem. Dev. Cell, 2016. 37(3): p. 254-66 [PMID:27134142] Cheng JZ,Zhou YP,Lv TX,Xie CP,Tian CE Research progress on the autonomous flowering time pathway in Arabidopsis. Physiol Mol Biol Plants, 2017. 23(3): p. 477-485 [PMID:28878488] Li C, et al. Verification of DNA motifs in Arabidopsis using CRISPR/Cas9-mediated mutagenesis. Plant Biotechnol. J., 2018. 16(8): p. 1446-1451 [PMID:29331085] Wang X, et al. The H3K27me3 demethylase REF6 promotes leaf senescence through directly activating major senescence regulatory and functional genes in Arabidopsis. PLoS Genet., 2019. 15(4): p. e1008068 [PMID:30969965] Qiu Q, et al. DNA methylation repels targeting of Arabidopsis REF6. Nat Commun, 2019. 10(1): p. 2063 [PMID:31048693]