PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G04150.1

Common Name

BHLH101, EN10, F21E1.70

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 240aa MW: 27716.5 Da PI: 10.3527

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G04150.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	41.3	2.7e-13	66	115	1	52
CHHHHHHHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIk 52 ++ +hn+ Er RR+++N ++ Lr llP + +++Kls +++ ++v+YI AT5G04150.1 66 KKLNHNASERDRRRKLNALYSSLRALLPLsD---QKRKLSIPMTVARVVKYIP 115 6789***********************66...9999************6 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	7.72E-13	65	136	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	2.23E-10	65	122	No hit	No description
PROSITE profile	PS50888	14.056	65	117	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	5.7E-10	66	122	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.5E-10	66	115	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	2.4E-8	71	123	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006357	Biological Process	regulation of transcription from RNA polymerase II promoter
GO:0010106	Biological Process	cellular response to iron ion starvation
GO:0055072	Biological Process	iron ion homeostasis
GO:0090575	Cellular Component	RNA polymerase II transcription factor complex
GO:0000977	Molecular Function	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0009005	anatomy	root
PO:0009025	anatomy	vascular leaf
PO:0007115	developmental stage	LP.04 four leaves visible stage

Sequence ? help Back to Top
Protein Sequence Length: 240 aa Download sequence Send to blast
MEYPWLQSQV HSFSPTLHFP SFLHPLDDSK SHNINLHHMS LSHSNNTNSN NNNYQEEDRG 60 AVVLEKKLNH NASERDRRRK LNALYSSLRA LLPLSDQKRK LSIPMTVARV VKYIPEQKQE 120 LQRLSRRKEE LLKRISRKTH QEQLRNKAMM DSIDSSSSQR IAANWLTDTE IAVQIATSKW 180 TSVSDMLLRL EENGLNVISV SSSVSSTARI FYTLHLQMRG DCKVRLEELI NGMLLGLRQS

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	245692_at	0.0
Expression Atlas	AT5G04150	-
AtGenExpress	AT5G04150	-
ATTED-II	AT5G04150	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Flowers. {ECO:0000269\|PubMed:12679534}.

Function -- GeneRIF ? help Back to Top
up-regulated by iron deficiency in roots and leaves [PMID: 17516080] bHLH100 and bHLH101 are key regulators of iron-deficiency responses independent of the master regulator FIT [PMID: 22984573] acts as a regulatory component in photo-oxidative stress responses [PMID: 30080593]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G04150.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up regulated by iron deficiency in roots and leaves, as well as by nickel, high zinc or high copper treatments. Repressed by high iron, low copper and low zinc treatments. {ECO:0000269\|PubMed:17516080}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT5G11260

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G04150

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AJ519810	0.0	AJ519810.1 Arabidopsis thaliana mRNA for putative bHLH transcription factor.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_196035.2	1e-176	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Swissprot	Q9FYE6	1e-177	BH101_ARATH; Transcription factor bHLH101
TrEMBL	A0A2H1ZE47	1e-174	A0A2H1ZE47_ARATH; Basic helix-loop-helix (BHLH) DNA-binding superfamily protein
STRING	AT5G04150.1	1e-176	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM482	28	153
Representative plant	OGRP2826	11	29

Link Out ? help Back to Top
Phytozome	AT5G04150.1
Entrez Gene	830293
iHOP	AT5G04150
wikigenes	AT5G04150

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Underwood BA,Vanderhaeghen R,Whitford R,Town CD,Hilson P Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations. Plant Biotechnol. J., 2006. 4(3): p. 317-24 [PMID:17147637] Vorwieger A, et al. Iron assimilation and transcription factor controlled synthesis of riboflavin in plants. Planta, 2007. 226(1): p. 147-58 [PMID:17260143] Wang HY, et al. Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana. Planta, 2007. 226(4): p. 897-908 [PMID:17516080] Gong W, et al. The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors. Mol Plant, 2008. 1(1): p. 27-41 [PMID:19802365] Golisz A,Sugano M,Hiradate S,Fujii Y Microarray analysis of Arabidopsis plants in response to allelochemical L-DOPA. Planta, 2011. 233(2): p. 231-40 [PMID:20978802] Ivanov R,Brumbarova T,Bauer P Fitting into the harsh reality: regulation of iron-deficiency responses in dicotyledonous plants. Mol Plant, 2012. 5(1): p. 27-42 [PMID:21873619] Wang N, et al. Requirement and functional redundancy of Ib subgroup bHLH proteins for iron deficiency responses and uptake in Arabidopsis thaliana. Mol Plant, 2013. 6(2): p. 503-13 [PMID:22983953] Sivitz AB,Hermand V,Curie C,Vert G Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway. PLoS ONE, 2012. 7(9): p. e44843 [PMID:22984573] Maurer F,Naranjo Arcos MA,Bauer P Responses of a triple mutant defective in three iron deficiency-induced Basic Helix-Loop-Helix genes of the subgroup Ib(2) to iron deficiency and salicylic acid. PLoS ONE, 2014. 9(6): p. e99234 [PMID:24919188] Martínez-Trujillo M, et al. Chromate alters root system architecture and activates expression of genes involved in iron homeostasis and signaling in Arabidopsis thaliana. Plant Mol. Biol., 2014. 86(1-2): p. 35-50 [PMID:24928490] Li X,Zhang H,Ai Q,Liang G,Yu D Two bHLH Transcription Factors, bHLH34 and bHLH104, Regulate Iron Homeostasis in Arabidopsis thaliana. Plant Physiol., 2016. 170(4): p. 2478-93 [PMID:26921305] Van Dingenen J, et al. Strobilurins as growth-promoting compounds: how Stroby regulates Arabidopsis leaf growth. Plant Cell Environ., 2017. 40(9): p. 1748-1760 [PMID:28444690] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Kailasam S,Wang Y,Lo JC,Chang HF,Yeh KC S-Nitrosoglutathione works downstream of nitric oxide to mediate iron-deficiency signaling in Arabidopsis. Plant J., 2018. 94(1): p. 157-168 [PMID:29396986] Kurt F,Filiz E Genome-wide and comparative analysis of bHLH38, bHLH39, bHLH100 and bHLH101 genes in Arabidopsis, tomato, rice, soybean and maize: insights into iron (Fe) homeostasis. Biometals, 2018. 31(4): p. 489-504 [PMID:29546482] Noshi M, et al. Clade Ib basic helix-loop-helix transcription factor, bHLH101, acts as a regulatory component in photo-oxidative stress responses. Plant Sci., 2018. 274: p. 101-108 [PMID:30080593]