PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 284aa MW: 31867.7 Da PI: 8.4457

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT3G19860.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	27.5	5.7e-09	7	55	2	54
HHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS HLH 2 rrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 + + rE+ RR+++N+ f eL +l + +K++Ka+iL+ +v+ +k+L AT3G19860.1 7 KSQKAGREKLRREKLNEHFVELGNVLDPE----RPKNDKATILTDTVQLLKEL 55 5566779****************9987....69*************998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	13.268	5	55	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	1.1E-12	5	78	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	3.53E-13	5	71	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	2.86E-6	6	59	No hit	No description
Pfam	PF00010	9.1E-7	7	55	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	2.4E-6	11	61	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001016	developmental stage	L mature pollen stage
PO:0001017	developmental stage	M germinated pollen stage
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 284 aa Download sequence Send to blast
MDVSARKSQK AGREKLRREK LNEHFVELGN VLDPERPKND KATILTDTVQ LLKELTSEVN 60 KLKSEYTALT DESRELTQEK NDLREEKTSL KSDIENLNLQ YQQRLRSMSP WGAAMDHTVM 120 MAPPPSFPYP MPIAMPPGSI PMHPSMPSYT YFGNQNPSMI PAPCPTYMPY MPPNTVVEQQ 180 SVHIPQNPGN RSREPRAKVS RESRSEKAED SNEVATQLEL KTPGSTSDKD TLQRPEKTKR 240 CKRNNNNNSI EESSHSSKCS SSPSVRDHSS SSSVAGGQKP DDAK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.20167	0.0	bud\| flower\| leaf\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	186510253	0.0
Genevisible	257990_at	0.0
Expression Atlas	AT3G19860	-
AtGenExpress	AT3G19860	-
ATTED-II	AT3G19860	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems, and flowers. {ECO:0000269\|PubMed:12679534}.

Function -- GeneRIF ? help Back to Top
Data indicate that the transcript levels of HAK5 and four transcriptin factor genes DDF2, JLO, bHLH121 and TFII_A were highly up-regulated in response to both -K and salt stress. [PMID: 23825216]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT3G19860.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Interaction ? help Back to Top
Source	Intact With
IntAct	Search Q9LT23

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT3G19860

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK317507	0.0	AK317507.1 Arabidopsis thaliana AT3G19860 mRNA, complete cds, clone: RAFL25-09-J11.
GenBank	AY065107	0.0	AY065107.1 Arabidopsis thaliana unknown protein (At3g19860; MPN9.10) mRNA, complete cds.
GenBank	AY081703	0.0	AY081703.1 Arabidopsis thaliana unknown protein (At3g19860) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_188620.1	0.0	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Swissprot	Q9LT23	0.0	BH121_ARATH; Transcription factor bHLH121
TrEMBL	A0A1I9LN90	0.0	A0A1I9LN90_ARATH; Basic helix-loop-helix (BHLH) DNA-binding superfamily protein
STRING	AT3G19860.2	0.0	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT3G19860.1
Entrez Gene	821523
iHOP	AT3G19860
wikigenes	AT3G19860

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Yang KY,Kim YM,Lee S,Song PS,Soh MS Overexpression of a mutant basic helix-loop-helix protein HFR1, HFR1-deltaN105, activates a branch pathway of light signaling in Arabidopsis. Plant Physiol., 2003. 133(4): p. 1630-42 [PMID:14645731] Czechowski T,Bari RP,Stitt M,Scheible WR,Udvardi MK Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. Plant J., 2004. 38(2): p. 366-79 [PMID:15078338] Ehlting J, et al. Comparative transcriptome analysis of Arabidopsis thaliana infested by diamond back moth (Plutella xylostella) larvae reveals signatures of stress response, secondary metabolism, and signalling. BMC Genomics, 2008. 9: p. 154 [PMID:18400103] Long TA, et al. The bHLH transcription factor POPEYE regulates response to iron deficiency in Arabidopsis roots. Plant Cell, 2010. 22(7): p. 2219-36 [PMID:20675571] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Hong JP, et al. Identification and characterization of transcription factors regulating Arabidopsis HAK5. Plant Cell Physiol., 2013. 54(9): p. 1478-90 [PMID:23825216] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Min JH, et al. Arabidopsis Basic Helix-Loop-Helix 34 (bHLH34) Is Involved in Glucose Signaling through Binding to a GAGA Cis-Element. Front Plant Sci, 2017. 8: p. 2100 [PMID:29321786]