PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

TALE

Protein Properties

Length: 584aa MW: 65775.4 Da PI: 8.2483

Description

BEL1-like homeodomain 8

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT2G27990.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	Homeobox	30.2	7.4e-10	449	481	22	54
SSS--HHHHHHHHHHCTS-HHHHHHHHHHHHHH CS Homeobox 22 nrypsaeereeLAkklgLterqVkvWFqNrRak 54 ++yp+ ++++ LA+++gL+ +qV++WF N R + AT2G27990.1 449 HPYPTDSDKQMLATQTGLSRNQVSNWFINARVR 481 89*****************************88 PP
2	BELL	102.2	3.7e-33	319	387	4	72
BELL 4 elqkkkakLlslleeVdkrYkqyveqlqtvissFeavaglgsakpYtslAlkaiSrhFrcLkdaiaeqi 72 +++ kkakLl l+eeV+k+Yk y++qlqtv+ssF++vagl++a+pY+slAlk++Sr+F++L++aiae++ AT2G27990.1 319 KNRLKKAKLLFLQEEVCKWYKLYNHQLQTVMSSFNTVAGLNTATPYISLALKRTSRSFKALRTAIAEHV 387 5789**************************************************************986 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00574	1.4E-63	257	385	IPR006563	POX domain
Pfam	PF07526	6.0E-28	262	384	IPR006563	POX domain
PROSITE profile	PS50071	12.017	422	485	IPR001356	Homeobox domain
SMART	SM00389	1.4E-10	425	489	IPR001356	Homeobox domain
CDD	cd00086	2.74E-11	425	486	No hit	No description
SuperFamily	SSF46689	6.42E-17	427	491	IPR009057	Homeodomain-like
Gene3D	G3DSA:1.10.10.60	1.0E-27	428	489	IPR009057	Homeodomain-like
Pfam	PF05920	1.5E-18	442	481	IPR008422	Homeobox KN domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010076	Biological Process	maintenance of floral meristem identity
GO:0010077	Biological Process	maintenance of inflorescence meristem identity
GO:0010223	Biological Process	secondary shoot formation
GO:0010228	Biological Process	vegetative to reproductive phase transition of meristem
GO:0010229	Biological Process	inflorescence development
GO:0048645	Biological Process	organ formation
GO:0080006	Biological Process	internode patterning
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000037	anatomy	shoot apex
PO:0000229	anatomy	flower meristem
PO:0000230	anatomy	inflorescence meristem
PO:0008016	anatomy	vegetative shoot apical meristem
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0020100	anatomy	hypocotyl
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 584 aa Download sequence Send to blast
MDMIKPDFQQ IRRDKFRVEQ MNDFPNTWTQ QQHQNIRIPN NLDLIGILQN QISVPVQTDL 60 YQDSAATFMN MPQSIHRDPQ GPSNWRISDL SQPSTVNHGY DQAGIRPNNV ADLLSDHFSS 120 RNQILDRPLY VGRDSIPQSS MIRRSEVSCL DDNQKGCVTV ACSGTGNEIL RSSYDQGSSS 180 GSYRGEFSFL PSLENQSVAH NASNWNHGPV NVTATSHTNS KKGFPLSLLS DIPPSRDVGN 240 AAVLSTMNIH GPLGPFTGYA SILKSSRFLE PAQKMLEEFC ISYASKIISR SESTSMEDDD 300 DDDDNLSGFS SSSEPLEPKN RLKKAKLLFL QEEVCKWYKL YNHQLQTVMS SFNTVAGLNT 360 ATPYISLALK RTSRSFKALR TAIAEHVKQI SSHSSNGNNN NRFQKRQRSL IGNNVGFESQ 420 QQHIWRPQRG LPERAVAVLR AWLFDHFLHP YPTDSDKQML ATQTGLSRNQ VSNWFINARV 480 RLWKPMVEEI HTLETKAIKN ADTSHNIEPS NRPNTVSSPS HEQTLTGLSG TKRSRLEYMD 540 MVGFNRGNVS LTLELRRGVD NVIQTQTQDH QFGTGSQMFH DFVG

Sequence ? help Back to Top

Protein Sequence Length: 584 aa Download sequence Send to blast

MDMIKPDFQQ IRRDKFRVEQ MNDFPNTWTQ QQHQNIRIPN NLDLIGILQN QISVPVQTDL  60
YQDSAATFMN MPQSIHRDPQ GPSNWRISDL SQPSTVNHGY DQAGIRPNNV ADLLSDHFSS  120
RNQILDRPLY VGRDSIPQSS MIRRSEVSCL DDNQKGCVTV ACSGTGNEIL RSSYDQGSSS  180
GSYRGEFSFL PSLENQSVAH NASNWNHGPV NVTATSHTNS KKGFPLSLLS DIPPSRDVGN  240
AAVLSTMNIH GPLGPFTGYA SILKSSRFLE PAQKMLEEFC ISYASKIISR SESTSMEDDD  300
DDDDNLSGFS SSSEPLEPKN RLKKAKLLFL QEEVCKWYKL YNHQLQTVMS SFNTVAGLNT  360
ATPYISLALK RTSRSFKALR TAIAEHVKQI SSHSSNGNNN NRFQKRQRSL IGNNVGFESQ  420
QQHIWRPQRG LPERAVAVLR AWLFDHFLHP YPTDSDKQML ATQTGLSRNQ VSNWFINARV  480
RLWKPMVEEI HTLETKAIKN ADTSHNIEPS NRPNTVSSPS HEQTLTGLSG TKRSRLEYMD  540
MVGFNRGNVS LTLELRRGVD NVIQTQTQDH QFGTGSQMFH DFVG

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
3k2a_A	2e-15	431	489	5	63	Homeobox protein Meis2
3k2a_B	2e-15	431	489	5	63	Homeobox protein Meis2
Search in ModeBase

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	264068_at	0.0
Expression Atlas	AT2G27990	-
AtGenExpress	AT2G27990	-
ATTED-II	AT2G27990	-

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a BEL1-like homeobox gene that functions together with PNY in meristem maintenance by regulating the allocation process during vegetative and reproductive development. Both gene products are required for the competence of the SAM to respond properly to floral inductive signals.
UniProt	Required for specifying floral primordia and establishing early internode patterning events during inflorescence development. {ECO:0000269\|PubMed:15120075, ECO:0000269\|PubMed:16741748}.

Function -- GeneRIF ? help Back to Top
PNF functions in the inner whorls to regulate flower patterning events. [PNF] [PMID: 19082619] combined lesions in ATH1, PNY and PNF result in a full phenocopy of shoot apical meristem loss-of-function mutants. [PMID: 19175771] PNY and PNF act to restrict organogenesis to the peripheral zone by maintaining a boundary between the central zone and peripheral zone. [PMID: 21505100] SPL3, SPL4, and SPL5 function is dependent upon PNY and PNF [PMID: 21653282] Regulation of meristem central zone integrity by PNY and PNF is crucial for vegetative and reproductive development. [PMID: 21822063] The data reveal a potential mechanism by which repression of lateral organ boundary genes by PNY-PNF is essential for flowering. [PMID: 26417006]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT2G27990.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G26310(A), AT1G69120(A), AT5G61850(A)

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT4G08150, AT1G70510
IntAct	Search Q9SJJ3

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT2G27990

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK119153	0.0	AK119153.1 Arabidopsis thaliana At2g27990 mRNA for putative homeodomain transcription factor, complete cds, clone: RAFL21-50-D24.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_180366.1	0.0	BEL1-like homeodomain 8
Swissprot	Q9SJJ3	0.0	BLH8_ARATH; BEL1-like homeodomain protein 8
TrEMBL	A0A178VN67	0.0	A0A178VN67_ARATH; PNF
STRING	AT2G27990.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM14321	15	20
Representative plant	OGRP133	16	172

Link Out ? help Back to Top
Phytozome	AT2G27990.1
Entrez Gene	817343
iHOP	AT2G27990
wikigenes	AT2G27990

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Seki M, et al. Functional annotation of a full-length Arabidopsis cDNA collection. Science, 2002. 296(5565): p. 141-5 [PMID:11910074] Byrne ME,Groover AT,Fontana JR,Martienssen RA Phyllotactic pattern and stem cell fate are determined by the Arabidopsis homeobox gene BELLRINGER. Development, 2003. 130(17): p. 3941-50 [PMID:12874117] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Bhatt AM,Etchells JP,Canales C,Lagodienko A,Dickinson H VAAMANA--a BEL1-like homeodomain protein, interacts with KNOX proteins BP and STM and regulates inflorescence stem growth in Arabidopsis. Gene, 2004. 328: p. 103-11 [PMID:15019989] Smith HM,Campbell BC,Hake S Competence to respond to floral inductive signals requires the homeobox genes PENNYWISE and POUND-FOOLISH. Curr. Biol., 2004. 14(9): p. 812-7 [PMID:15120075] Hackbusch J,Richter K,Müller J,Salamini F,Uhrig JF A central role of Arabidopsis thaliana ovate family proteins in networking and subcellular localization of 3-aa loop extension homeodomain proteins. Proc. Natl. Acad. Sci. U.S.A., 2005. 102(13): p. 4908-12 [PMID:15781858] Kanrar S,Onguka O,Smith HM Arabidopsis inflorescence architecture requires the activities of KNOX-BELL homeodomain heterodimers. Planta, 2006. 224(5): p. 1163-73 [PMID:16741748] Kanrar S,Bhattacharya M,Arthur B,Courtier J,Smith HM Regulatory networks that function to specify flower meristems require the function of homeobox genes PENNYWISE and POUND-FOOLISH in Arabidopsis. Plant J., 2008. 54(5): p. 924-37 [PMID:18298668] Li Y, et al. Genome-wide identification of osmotic stress response gene in Arabidopsis thaliana. Genomics, 2008. 92(6): p. 488-93 [PMID:18804526] Yu L,Patibanda V,Smith HM A novel role of BELL1-like homeobox genes, PENNYWISE and POUND-FOOLISH, in floral patterning. Planta, 2009. 229(3): p. 693-707 [PMID:19082619] Jackson SD Plant responses to photoperiod. New Phytol., 2009. 181(3): p. 517-31 [PMID:19154317] Rutjens B, et al. Shoot apical meristem function in Arabidopsis requires the combined activities of three BEL1-like homeodomain proteins. Plant J., 2009. 58(4): p. 641-54 [PMID:19175771] Smith HM,Ung N,Lal S,Courtier J Specification of reproductive meristems requires the combined function of SHOOT MERISTEMLESS and floral integrators FLOWERING LOCUS T and FD during Arabidopsis inflorescence development. J. Exp. Bot., 2011. 62(2): p. 583-93 [PMID:20937733] Ung N,Lal S,Smith HM The role of PENNYWISE and POUND-FOOLISH in the maintenance of the shoot apical meristem in Arabidopsis. Plant Physiol., 2011. 156(2): p. 605-14 [PMID:21505100] Lal S,Pacis LB,Smith HM Regulation of the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE genes/microRNA156 module by the homeodomain proteins PENNYWISE and POUND-FOOLISH in Arabidopsis. Mol Plant, 2011. 4(6): p. 1123-32 [PMID:21653282] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Ung N,Smith HM Regulation of shoot meristem integrity during Arabidopsis vegetative development. Plant Signal Behav, 2011. 6(8): p. 1250-2 [PMID:21822063] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Khan M, et al. Repression of Lateral Organ Boundary Genes by PENNYWISE and POUND-FOOLISH Is Essential for Meristem Maintenance and Flowering in Arabidopsis. Plant Physiol., 2015. 169(3): p. 2166-86 [PMID:26417006]