PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Sisymbrieae; Sisymbrium

Family

WRKY

Protein Properties

Length: 487aa MW: 53645.2 Da PI: 4.7751

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
676756144	genome	VEGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	101.3	5.5e-32	300	357	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 dDg++WrKYGqK+vkg+++prsYY+Ct++gC+vkk+ver a+d ++v++tY g H+h+ 676756144 300 DDGFRWRKYGQKVVKGNPNPRSYYKCTHNGCNVKKHVERGADDVTLVVTTYDGVHEHP 357 8********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	3.4E-33	284	359	IPR003657	WRKY domain
SuperFamily	SSF118290	3.01E-27	293	359	IPR003657	WRKY domain
PROSITE profile	PS50811	32.707	294	359	IPR003657	WRKY domain
SMART	SM00774	1.2E-36	299	358	IPR003657	WRKY domain
Pfam	PF03106	1.7E-24	300	357	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009960	Biological Process	endosperm development
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 487 aa Download sequence Send to blast
MSDCDDGNFM EMASYWAPPP TPSPRALFAM LNQTDYGLNL SSDIFPQTNV STDHKQPEQI 60 SGLSGRVAAR AGFNLAPLET ENLSPFAALF RNPTTVPSPV VAISPGFSPS ALLQTPNNFS 120 HASSQFTPLS PVANDGLPET VENSGDHHAT TMTINNDVPH QPMHVNAPPQ EEGSDVIPTE 180 DSVHVPSHES HVDPIGARLV ASFESEVVDD TDIINLISLD NESEDEDKDK VKDKDKDKDK 240 DEDEGVDENK DKDDNIVDEP EVEPSPKRRK FDVSNMIGAT RTSRNQRIII QMESEEDHPD 300 DGFRWRKYGQ KVVKGNPNPR SYYKCTHNGC NVKKHVERGA DDVTLVVTTY DGVHEHPAPA 360 ARSSSSGSRT RSSASMSQEQ SKRISRLGRV QPPPSSSAPQ DMTPFPPSSA SAPLDMRPFT 420 SSLDARADMT QLFMTGLAKL PSLPVNQNHG FMYRRNDEPR VDYVVPDGTE VYKGIMHRLF 480 VNFGVNL

Sequence ? help Back to Top

Protein Sequence Length: 487 aa Download sequence Send to blast

MSDCDDGNFM EMASYWAPPP TPSPRALFAM LNQTDYGLNL SSDIFPQTNV STDHKQPEQI  60
SGLSGRVAAR AGFNLAPLET ENLSPFAALF RNPTTVPSPV VAISPGFSPS ALLQTPNNFS  120
HASSQFTPLS PVANDGLPET VENSGDHHAT TMTINNDVPH QPMHVNAPPQ EEGSDVIPTE  180
DSVHVPSHES HVDPIGARLV ASFESEVVDD TDIINLISLD NESEDEDKDK VKDKDKDKDK  240
DEDEGVDENK DKDDNIVDEP EVEPSPKRRK FDVSNMIGAT RTSRNQRIII QMESEEDHPD  300
DGFRWRKYGQ KVVKGNPNPR SYYKCTHNGC NVKKHVERGA DDVTLVVTTY DGVHEHPAPA  360
ARSSSSGSRT RSSASMSQEQ SKRISRLGRV QPPPSSSAPQ DMTPFPPSSA SAPLDMRPFT  420
SSLDARADMT QLFMTGLAKL PSLPVNQNHG FMYRRNDEPR VDYVVPDGTE VYKGIMHRLF  480
VNFGVNL

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	2e-25	290	360	8	78	Probable WRKY transcription factor 4
2lex_A	2e-25	290	360	8	78	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Modulates seed size by negatively regulating the cellularization of syncytial endosperm (PubMed:16293693). {ECO:0000250\|UniProtKB:Q9SI37, ECO:0000269\|PubMed:16293693}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	676756144

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006392618.1	0.0	probable WRKY transcription factor 10
Swissprot	Q9LG05	1e-148	WRK10_ARATH; Probable WRKY transcription factor 10
TrEMBL	V4KFE8	0.0	V4KFE8_EUTSA; Uncharacterized protein
STRING	XP_006392618.1	0.0	(Eutrema salsugineum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM10299	17	33

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G55600.1	1e-142	WRKY DNA-binding protein 10

Publications ? help Back to Top
Li J,Nie X,Tan JL,Berger F Integration of epigenetic and genetic controls of seed size by cytokinin in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2013. 110(38): p. 15479-84 [PMID:24003120] Fatihi A,Zbierzak AM,Dörmann P Alterations in seed development gene expression affect size and oil content of Arabidopsis seeds. Plant Physiol., 2013. 163(2): p. 973-85 [PMID:24014578] Liu P, et al. Arabidopsis RAN1 mediates seed development through its parental ratio by affecting the onset of endosperm cellularization. Mol Plant, 2014. 7(8): p. 1316-1328 [PMID:24719465] Xiao YG,Sun QB,Kang XJ,Chen CB,Ni M SHORT HYPOCOTYL UNDER BLUE1 or HAIKU2 mixepression alters canola and Arabidopsis seed development. New Phytol., 2016. 209(2): p. 636-49 [PMID:26389843] Meng LS,Wang YB,Loake GJ,Jiang JH Seed Embryo Development Is Regulated via an AN3-MINI3 Gene Cascade. Front Plant Sci, 2016. 7: p. 1645 [PMID:27857719] Meng LS,Wang YB,Loake GJ,Jiang JH Corrigendum: Seed Embryo Development Is Regulated via an AN3-MINI3 Gene Cascade. Front Plant Sci, 2017. 8: p. 1073 [PMID:28616014]