PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Sisymbrieae; Sisymbrium

Family

WRKY

Protein Properties

Length: 550aa MW: 60174.6 Da PI: 8.3531

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
676750298	genome	VEGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	104.1	7.4e-33	283	339	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 dDgynWrKYGqK+vkgs+ prsYY+Ct++ Cpvkkkvers d++v+ei+Y+g+H h+ 676750298 283 DDGYNWRKYGQKQVKGSDSPRSYYKCTHPACPVKKKVERSL-DGQVTEIIYKGQHSHD 339 8**************************************.*************7 PP
2	WRKY	105	3.9e-33	450	508	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+vkg+++prsYY+Ct++ C v+k+ver+a+dpk+v++tYeg+Hnh+ 676750298 450 LDDGYRWRKYGQKVVKGNPYPRSYYKCTTPACGVRKHVERAANDPKAVVTTYEGKHNHD 508 59********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.6E-28	274	341	IPR003657	WRKY domain
PROSITE profile	PS50811	23.629	277	341	IPR003657	WRKY domain
SuperFamily	SSF118290	3.4E-25	280	341	IPR003657	WRKY domain
SMART	SM00774	3.0E-35	282	340	IPR003657	WRKY domain
Pfam	PF03106	6.2E-25	283	339	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	3.1E-36	435	510	IPR003657	WRKY domain
SuperFamily	SSF118290	3.4E-29	442	510	IPR003657	WRKY domain
PROSITE profile	PS50811	37.39	445	510	IPR003657	WRKY domain
SMART	SM00774	6.9E-38	450	509	IPR003657	WRKY domain
Pfam	PF03106	1.7E-25	451	508	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 550 aa Download sequence Send to blast
MAKKEEEEEQ QQEQEQPLTL KPSTGGASRS RPTISLPPRP FGEMFFSGGV GFSGFSPGPM 60 TLVSNLFSDP DEFKSFSQLL AGAMASPAAA VAAAAVVASA HQTSVSSVGG NGDNGGDVDP 120 RFKQNRPTGL MITQPPAMFT VPPGLSPATL LDSPSFFGLF SPIQVKIREK FDFDELRMFL 180 CILKVGNFQT KGTFGMTHQQ ALAQVTAQAV QGSNVQMQSQ SEFPCTTQQQ ETSLTEVLPS 240 SSSGPRSQIP APVRDSSQAQ RGTLEVSVYE HRSQPQNADK PADDGYNWRK YGQKQVKGSD 300 SPRSYYKCTH PACPVKKKVE RSLDGQVTEI IYKGQHSHDP PQNTKRGGNN NGSSKSSDIA 360 TQFQTSNSSL NKTKRDQETS QVTTTEQMSE ASDSEEVGNA DTSVGRNHED EPDPKRRNVE 420 GRVSEPASST HRTVTEPRII VQTTSEVDLL DDGYRWRKYG QKVVKGNPYP RSYYKCTTPA 480 CGVRKHVERA ANDPKAVVTT YEGKHNHDVP AARTSSHQLR PNNNIHNTSA VNLNQQQAVA 540 RLRLKEEQIT

Sequence ? help Back to Top

Protein Sequence Length: 550 aa Download sequence Send to blast

MAKKEEEEEQ QQEQEQPLTL KPSTGGASRS RPTISLPPRP FGEMFFSGGV GFSGFSPGPM  60
TLVSNLFSDP DEFKSFSQLL AGAMASPAAA VAAAAVVASA HQTSVSSVGG NGDNGGDVDP  120
RFKQNRPTGL MITQPPAMFT VPPGLSPATL LDSPSFFGLF SPIQVKIREK FDFDELRMFL  180
CILKVGNFQT KGTFGMTHQQ ALAQVTAQAV QGSNVQMQSQ SEFPCTTQQQ ETSLTEVLPS  240
SSSGPRSQIP APVRDSSQAQ RGTLEVSVYE HRSQPQNADK PADDGYNWRK YGQKQVKGSD  300
SPRSYYKCTH PACPVKKKVE RSLDGQVTEI IYKGQHSHDP PQNTKRGGNN NGSSKSSDIA  360
TQFQTSNSSL NKTKRDQETS QVTTTEQMSE ASDSEEVGNA DTSVGRNHED EPDPKRRNVE  420
GRVSEPASST HRTVTEPRII VQTTSEVDLL DDGYRWRKYG QKVVKGNPYP RSYYKCTTPA  480
CGVRKHVERA ANDPKAVVTT YEGKHNHDVP AARTSSHQLR PNNNIHNTSA VNLNQQQAVA  540
RLRLKEEQIT

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	7e-44	283	511	8	78	Probable WRKY transcription factor 4
2lex_A	7e-44	283	511	8	78	Probable WRKY transcription factor 4
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Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00260	DAP	Transfer from AT2G03340	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	676750298

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid and during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006395712.1	0.0	probable WRKY transcription factor 3
Swissprot	Q9ZQ70	0.0	WRKY3_ARATH; Probable WRKY transcription factor 3
TrEMBL	V4KZY4	0.0	V4KZY4_EUTSA; Uncharacterized protein
STRING	XP_006395712.1	0.0	(Eutrema salsugineum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1534	27	92

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G03340.1	0.0	WRKY DNA-binding protein 3

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Aamir M, et al. Structural and Functional Insights into WRKY3 and WRKY4 Transcription Factors to Unravel the WRKY-DNA (W-Box) Complex Interaction in Tomato (Solanum lycopersicum L.). A Computational Approach. Front Plant Sci, 2017. 8: p. 819 [PMID:28611792]