PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Sisymbrieae; Sisymbrium

Family

WRKY

Protein Properties

Length: 291aa MW: 32638.4 Da PI: 8.845

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
676740574	genome	VEGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	95.6	3.4e-30	157	215	1	58
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnh 58 ++Dgy+WrKYGqK+ ++++ pr+Y+rC++a +Cpvkkkv+rsaedp+v++ tYeg+Hnh 676740574 157 VKDGYQWRKYGQKVTRDNPSPRAYFRCSFApSCPVKKKVQRSAEDPSVLVATYEGTHNH 215 58*************************99************************** PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.2E-28	150	216	IPR003657	WRKY domain
SuperFamily	SSF118290	4.05E-25	154	216	IPR003657	WRKY domain
SMART	SM00774	4.7E-34	157	217	IPR003657	WRKY domain
Pfam	PF03106	1.1E-24	158	215	IPR003657	WRKY domain
PROSITE profile	PS50811	25.993	159	218	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0002237	Biological Process	response to molecule of bacterial origin
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009751	Biological Process	response to salicylic acid
GO:0010200	Biological Process	response to chitin
GO:0042742	Biological Process	defense response to bacterium
GO:0050691	Biological Process	regulation of defense response to virus by host
GO:0050832	Biological Process	defense response to fungus
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0042802	Molecular Function	identical protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 291 aa Download sequence Send to blast
MASHFQKKEV KASASPDSER KWLVKDESAS ELREELKRVN SENKKLTEML ATVCENYYAL 60 HQHLEKLQRR QSPEIDQIEQ PTKKRKQDSD DFLGFPNGFS SGKTENSSSN EEHHHLQQHD 120 QKNQLLSCKR PVTDTFNKAK VSTVYVPADS SDTSLTVKDG YQWRKYGQKV TRDNPSPRAY 180 FRCSFAPSCP VKKKVQRSAE DPSVLVATYE GTHNHLGPNA SEGDAISQAG SSTVTLDLVH 240 GGHRLALEKN ERGMMQEVLV QQMASSLTKD SKFTAALAAA ISGRLMEQSR T

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	6e-20	159	215	19	74	Probable WRKY transcription factor 4
2lex_A	6e-20	159	215	19	74	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Positively modulates defense-related gene expression and disease resistance. {ECO:0000269\|PubMed:12068113}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00465	DAP	Transfer from AT4G31800	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	676740574

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid and pathogens. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:12068113}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KF430067	0.0	KF430067.1 Brassica rapa WRKY transcription factor 60 (WRKY60) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018447976.1	1e-180	PREDICTED: WRKY transcription factor 18-like
Swissprot	Q9C5T4	1e-172	WRK18_ARATH; WRKY transcription factor 18
TrEMBL	V4P3M5	1e-178	V4P3M5_EUTSA; Uncharacterized protein
STRING	Bostr.7867s1032.1.p	0.0	(Boechera stricta)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM6584	19	45

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G31800.1	1e-174	WRKY DNA-binding protein 18

Publications ? help Back to Top
Keates SE,Kostman TA,Anderson JD,Bailey BA Altered gene expression in three plant species in response to treatment with Nep1, a fungal protein that causes necrosis. Plant Physiol., 2003. 132(3): p. 1610-22 [PMID:12857840] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Bülow L,Steffens NO,Galuschka C,Schindler M,Hehl R AthaMap: from in silico data to real transcription factor binding sites. In Silico Biol. (Gedrukt), 2006. 6(3): p. 243-52 [PMID:16922688] Deeken R, et al. Identification of Arabidopsis thaliana phloem RNAs provides a search criterion for phloem-based transcripts hidden in complex datasets of microarray experiments. Plant J., 2008. 55(5): p. 746-59 [PMID:18485061] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Zhang Y, et al. Regulation of oncogene expression in T-DNA-transformed host plant cells. PLoS Pathog., 2015. 11(1): p. e1004620 [PMID:25615824] Geilen K,Böhmer M Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana. Sci Rep, 2015. 5: p. 13369 [PMID:26293691] Geilen K,Böhmer M Dynamic subnuclear relocalization of WRKY40, a potential new mechanism of ABA-dependent transcription factor regulation. Plant Signal Behav, 2015. 10(11): p. e1106659 [PMID:26479147] Lu K, et al. Overexpression of an Arabidopsis cysteine-rich receptor-like protein kinase, CRK5, enhances abscisic acid sensitivity and confers drought tolerance. J. Exp. Bot., 2016. 67(17): p. 5009-27 [PMID:27406784] Birkenbihl RP,Kracher B,Roccaro M,Somssich IE Induced Genome-Wide Binding of Three Arabidopsis WRKY Transcription Factors during Early MAMP-Triggered Immunity. Plant Cell, 2017. 29(1): p. 20-38 [PMID:28011690]