PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Sisymbrieae; Sisymbrium

Family

WRKY

Protein Properties

Length: 311aa MW: 34896.1 Da PI: 6.6315

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
676728392	genome	VEGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	108.1	4.3e-34	165	222	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 +Dgy+WrKYGqK vk+s++prsYYrCt+++C+vkk+vers +dp+vv++tYeg+Hnh+ 676728392 165 EDGYRWRKYGQKAVKNSPYPRSYYRCTTQRCNVKKRVERSFQDPTVVITTYEGQHNHP 222 8********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PIRSF	PIRSF038130	6.8E-109	1	311	IPR017396	PWRKY transcription factor, group IIc
Gene3D	G3DSA:2.20.25.80	1.2E-35	149	224	IPR003657	WRKY domain
SuperFamily	SSF118290	1.2E-29	156	224	IPR003657	WRKY domain
PROSITE profile	PS50811	32.856	159	224	IPR003657	WRKY domain
SMART	SM00774	1.8E-39	164	223	IPR003657	WRKY domain
Pfam	PF03106	2.3E-26	165	222	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 311 aa Download sequence Send to blast
MSNETKDLHN YQYPSSFSLY EMMNFPTAPP SSYGNNGFDP SSYSFTDCLQ SSPGAYDSLL 60 QKNFGLSPSS SEVFNSSMDQ ESKRDVTNDV IGETPTRVSA SSSSSEADHP GEDSGKSQRK 120 RELVGDAGEE NQSSKKVGKT KKNEEKKQRE PRVSFMTKSE VDHLEDGYRW RKYGQKAVKN 180 SPYPRSYYRC TTQRCNVKKR VERSFQDPTV VITTYEGQHN HPIPTNLRGS SAAAAMYSAD 240 FMNPRSFTHD MFRSSAYTNG GSAAAALDYG YEQSGYASMN ANPNSCQEYH QGGEYELLKE 300 IFPSIFFKQE P

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	1e-29	155	224	8	77	Probable WRKY transcription factor 4
2lex_A	1e-29	155	224	8	77	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00440	DAP	Transfer from AT4G18170	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	676728392

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KF430045	0.0	KF430045.1 Brassica rapa WRKY transcription factor 28 (WRKY28) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013595930.1	0.0	PREDICTED: probable WRKY transcription factor 28
Swissprot	Q8VWJ2	0.0	WRK28_ARATH; WRKY transcription factor 28
TrEMBL	A0A0D3DET2	0.0	A0A0D3DET2_BRAOL; WRKY transcription factor
TrEMBL	A0A3P6ER93	0.0	A0A3P6ER93_BRAOL; WRKY transcription factor
STRING	Bo7g105800.1	0.0	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1524	26	93

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G18170.1	1e-174	WRKY DNA-binding protein 28

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Kim SH, et al. Characterization of a Novel DWD protein that participates in heat stress response in Arabidopsis. Mol. Cells, 2014. 37(11): p. 833-40 [PMID:25358503] Zhao L, et al. KLU suppresses megasporocyte cell fate through SWR1-mediated activation of WRKY28 expression in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2018. 115(3): p. E526-E535 [PMID:29288215]