PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 320aa MW: 35567.9 Da PI: 7.2314

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
493050	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	108	4.5e-34	172	229	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 +Dgy+WrKYGqK vk+s++prsYYrCt+++C+vkk+vers +dp+vv++tYeg+Hnh+ 493050 172 EDGYRWRKYGQKAVKNSPYPRSYYRCTTQRCNVKKRVERSFQDPTVVITTYEGQHNHP 229 8********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PIRSF	PIRSF038130	4.6E-114	1	319	IPR017396	PWRKY transcription factor, group IIc
Gene3D	G3DSA:2.20.25.80	1.2E-35	156	231	IPR003657	WRKY domain
SuperFamily	SSF118290	1.26E-29	163	231	IPR003657	WRKY domain
PROSITE profile	PS50811	32.856	166	231	IPR003657	WRKY domain
SMART	SM00774	1.8E-39	171	230	IPR003657	WRKY domain
Pfam	PF03106	2.4E-26	172	229	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 320 aa Download sequence Send to blast
MSNETRDLYN YQYPSSFSLH EMMNLPTSNP SSYGNISSQN GLYPSTYSFT DCLQSSPGAY 60 ESLLQKSFGL SPSSSEVFNS SIDQEPNRDV TNDVINGGAC NETETRVSAS NSSSSEADHP 120 GEDSGKSRRK RELVGEEDQS SKKVGKTKKN EVKKQREPRV SFMTKSEVDH LEDGYRWRKY 180 GQKAVKNSPY PRSYYRCTTQ RCNVKKRVER SFQDPTVVIT TYEGQHNHPI PTNLRGSSAA 240 AAMFSADLLT PRSFAHDMFR TAAYTNGGSA AAAALDYGYG QSGYGSVNAN PSSHQEYHQG 300 GEYELLREIF PSIFFKQEP*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	2e-29	162	231	8	77	Probable WRKY transcription factor 4
2lex_A	2e-29	162	231	8	77	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00440	DAP	Transfer from AT4G18170	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	493050

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF442393	0.0	AF442393.1 Arabidopsis thaliana WRKY transcription factor 28 (WRKY28) mRNA, complete cds.
GenBank	AY070761	0.0	AY070761.1 Arabidopsis thaliana AT4g18170/T9A21_10 mRNA, complete cds.
GenBank	AY116943	0.0	AY116943.1 Arabidopsis thaliana AT4g18170/T9A21_10 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020872179.1	0.0	probable WRKY transcription factor 28
Swissprot	Q8VWJ2	0.0	WRK28_ARATH; WRKY transcription factor 28
TrEMBL	D7MCV8	0.0	D7MCV8_ARALL; WRKY transcription factor
STRING	fgenesh2_kg.7__2483__AT4G18170.1	0.0	(Arabidopsis lyrata)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1524	26	93

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G18170.1	0.0	WRKY DNA-binding protein 28

Link Out ? help Back to Top
Phytozome	493050
Entrez Gene	9306137

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Kim SH, et al. Characterization of a Novel DWD protein that participates in heat stress response in Arabidopsis. Mol. Cells, 2014. 37(11): p. 833-40 [PMID:25358503] Zhao L, et al. KLU suppresses megasporocyte cell fate through SWR1-mediated activation of WRKY28 expression in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2018. 115(3): p. E526-E535 [PMID:29288215]