PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Chloridoideae; Eragrostideae; Eragrostidinae; Eragrostis

Family

WRKY

Protein Properties

Length: 227aa MW: 25157.8 Da PI: 8.2202

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
462940369	genome	Tef	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	103.7	9.8e-33	131	187	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 dDgynWrKYGqK vkg e+prsYY+Ct+++Cpvkkkversa d+++++i+Y+g+Hnh+ 462940369 131 DDGYNWRKYGQKAVKGGEYPRSYYKCTHSSCPVKKKVERSA-DGQITQIIYRGQHNHH 187 8**************************************.*************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.2E-29	122	189	IPR003657	WRKY domain
PROSITE profile	PS50811	24.443	125	189	IPR003657	WRKY domain
SuperFamily	SSF118290	3.01E-26	128	189	IPR003657	WRKY domain
SMART	SM00774	6.9E-34	130	188	IPR003657	WRKY domain
Pfam	PF03106	3.3E-25	131	187	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 227 aa Download sequence Send to blast
MVLLTISWHG VPVTLYATVL GELGFMDRKL DRQQFGLRGV GSFEMSHQQA LAQVTAQAVH 60 SPYNMVNQTD YSLPFSSTTT TSQNVSSSAN VASTSTKVIP IQPSDTGNSN IQSNEISQPQ 120 TSALTADKPA DDGYNWRKYG QKAVKGGEYP RSYYKCTHSS CPVKKKVERS ADGQITQIIY 180 RGQHNHHRPS KRRSKDGGEL LNEGDDFREN EDAPTRSEPG SELNREF

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	2e-18	131	190	18	78	Probable WRKY transcription factor 4
2lex_A	2e-18	131	190	18	78	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds specifically to the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Has a positive role in resistance to necrotrophic pathogens (e.g. Botrytis cinerea), but a negative effect on plant resistance to biotrophic pathogens (e.g. Pseudomonas syringae). {ECO:0000269\|PubMed:18570649, ECO:0000269\|PubMed:22219184}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	462940369

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By biotic and abiotic stresses such as pathogen infection (e.g. Botrytis cinerea and Pseudomonas syringae), salicylic acid (SA), jasmonic acid (JA), ethylene (ACC), liquid infiltration or spraying, and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756, ECO:0000269\|PubMed:18570649}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_012698576.1	6e-93	probable WRKY transcription factor 4 isoform X2
Refseq	XP_020400022.1	5e-93	probable WRKY transcription factor 4 isoform X2
Swissprot	Q9XI90	2e-45	WRKY4_ARATH; Probable WRKY transcription factor 4
TrEMBL	A0A0A9G7H5	2e-93	A0A0A9G7H5_ARUDO; Uncharacterized protein
STRING	OPUNC03G21490.1	8e-93	(Oryza punctata)
STRING	Sb01g032120.1	3e-92	(Sorghum bicolor)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP1345	38	122

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G01080.1	3e-37	WRKY DNA-binding protein 58

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Aamir M, et al. Structural and Functional Insights into WRKY3 and WRKY4 Transcription Factors to Unravel the WRKY-DNA (W-Box) Complex Interaction in Tomato (Solanum lycopersicum L.). A Computational Approach. Front Plant Sci, 2017. 8: p. 819 [PMID:28611792]