PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Chloridoideae; Eragrostideae; Eragrostidinae; Eragrostis

Family

WRKY

Protein Properties

Length: 170aa MW: 18764.2 Da PI: 7.3832

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
462861454	genome	Tef	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	91.8	5.5e-29	11	70	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dgy+WrKYGqK+ +++++pr+Y+rC++a +Cpvkkkv+rsaed +++ +YegeHnh 462861454 11 VKDGYQWRKYGQKVTRDNPYPRAYFRCAYApSCPVKKKVQRSAEDRLMLVAHYEGEHNHA 70 58*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	25.729	6	69	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	5.9E-28	6	71	IPR003657	WRKY domain
SuperFamily	SSF118290	3.79E-25	7	70	IPR003657	WRKY domain
SMART	SM00774	2.3E-33	11	71	IPR003657	WRKY domain
Pfam	PF03106	4.3E-24	12	69	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 170 aa Download sequence Send to blast
MNPADAEANL VKDGYQWRKY GQKVTRDNPY PRAYFRCAYA PSCPVKKKVQ RSAEDRLMLV 60 AHYEGEHNHA QCAPSELVSD ASTRQQGPSS GSLPCSISIN SLGRTITLGL TNQGSGSNEQ 120 AVVGEVAAPE FRKVMVDEMV TLLKNDSEFM ESLTSAVTAR MMEKLSDRIF

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	1e-21	9	69	12	71	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription repressor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Regulates, probably indirectly, the activation of defense-related genes during defense response (By similarity). Regulates negatively basal innate immunity and XA21-mediated defense against X.oryzae pv. oryzae (Xoo) (By similarity). {ECO:0000250\|UniProtKB:Q6EPZ0, ECO:0000250\|UniProtKB:Q6QHD1}.
UniProt	Transcription repressor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Regulates, probably indirectly, the activation of defense-related genes during defense response (By similarity). Regulates negatively basal innate immunity and XA21-mediated defense against X.oryzae pv. oryzae (Xoo) (PubMed:19825552, PubMed:21961049). {ECO:0000250\|UniProtKB:Q6QHD1, ECO:0000269\|PubMed:19825552, ECO:0000269\|PubMed:21961049}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	462861454

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Might be activated by XA21 in the nucleus upon pathogen infection (PubMed:19825552). Induced by biotic elicitors (e.g. fungal chitin oligosaccharide) (PubMed:23462973). Induced by pathogen infection (e.g. M.grisea and X.oryzae pv. oryzae (Xoo)) (PubMed:16528562, PubMed:24093634). Accumulates after treatment with benzothiadiazole (BTH) and salicylic acid (SA) (PubMed:17601827, PubMed:16528562, PubMed:24093634). Transactivated by WRKY45 (PubMed:24093634). {ECO:0000269\|PubMed:16528562, ECO:0000269\|PubMed:17601827, ECO:0000269\|PubMed:23462973, ECO:0000269\|PubMed:24093634, ECO:0000303\|PubMed:19825552}.
UniProt	INDUCTION: Might be activated by XA21 in the nucleus upon pathogen infection (PubMed:20118235). Induced by biotic elicitors (e.g. fungal chitin oligosaccharide) (By similarity). Induced by pathogen infection (e.g. M.grisea and X.oryzae pv. oryzae (Xoo)). Accumulates after treatment with benzothiadiazole (BTH) and salicylic acid (SA) (PubMed:16528562). Transactivated by WRKY45 (By similarity). {ECO:0000250\|UniProtKB:Q6EPZ0, ECO:0000269\|PubMed:16528562, ECO:0000303\|PubMed:20118235}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	BT035304	4e-44	BT035304.1 Zea mays full-length cDNA clone ZM_BFb0054B07 mRNA, complete cds.
GenBank	BT068493	4e-44	BT068493.1 Zea mays full-length cDNA clone ZM_BFb0234B18 mRNA, complete cds.
GenBank	EU518772	4e-44	EU518772.1 Zea mays WRKY DNA-binding protein (WRKY) mRNA, complete cds.
GenBank	EU955814	4e-44	EU955814.1 Zea mays clone 1547310 WRKY76 - superfamily of TFs having WRKY and zinc finger domains mRNA, complete cds.
GenBank	KJ726810	4e-44	KJ726810.1 Zea mays clone pUT3166 WRKY transcription factor (WRKY53) mRNA, partial cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_004956832.1	3e-86	WRKY transcription factor WRKY62
Swissprot	B8BF91	2e-66	WRK62_ORYSI; WRKY transcription factor WRKY62
Swissprot	Q6EPZ0	2e-66	WRK62_ORYSJ; WRKY transcription factor WRKY62
TrEMBL	K3ZW13	8e-85	K3ZW13_SETIT; Uncharacterized protein
STRING	Si030794m	1e-85	(Setaria italica)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP12921	28	34

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G31800.1	2e-42	WRKY DNA-binding protein 18

Publications ? help Back to Top
Kikuchi S, et al. Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice. Science, 2003. 301(5631): p. 376-9 [PMID:12869764] Zhang Y,Wang L The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol. Biol., 2005. 5: p. 1 [PMID:15629062] Wu KL,Guo ZJ,Wang HH,Li J The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res., 2005. 12(1): p. 9-26 [PMID:16106749] Chen X, et al. A conserved threonine residue in the juxtamembrane domain of the XA21 pattern recognition receptor is critical for kinase autophosphorylation and XA21-mediated immunity. J. Biol. Chem., 2010. 285(14): p. 10454-63 [PMID:20118235] Peng Y,Bartley LE,Canlas P,Ronald PC OsWRKY IIa Transcription Factors Modulate Rice Innate Immunity. Rice (N Y), 2010. 3(1): p. 36-42 [PMID:21961049] Park CJ,Ronald PC Cleavage and nuclear localization of the rice XA21 immune receptor. Nat Commun, 2012. 3: p. 920 [PMID:22735448] Chujo T, et al. OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus. Plant Mol. Biol., 2013. 82(1-2): p. 23-37 [PMID:23462973] Nakayama A, et al. Genome-wide identification of WRKY45-regulated genes that mediate benzothiadiazole-induced defense responses in rice. BMC Plant Biol., 2013. 13: p. 150 [PMID:24093634] Hou Y, et al. JMJ704 positively regulates rice defense response against Xanthomonas oryzae pv. oryzae infection via reducing H3K4me2/3 associated with negative disease resistance regulators. BMC Plant Biol., 2015. 15: p. 286 [PMID:26646110] Liu J, et al. Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense. Plant Physiol., 2016. 171(2): p. 1427-42 [PMID:27208272]