PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Chloridoideae; Eragrostideae; Eragrostidinae; Eragrostis

Family

WRKY

Protein Properties

Length: 118aa MW: 13068.1 Da PI: 10.4228

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
462847106	genome	Tef	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	67.9	1.5e-21	28	79	1	50
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---E.EEEEE-SSSTTEEEE CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvk.kkversaedpkvvei 50 ++Dgy+WrKYGqK+ k++++pr+Y+rC++a Cpvk k+ve +a + e+ 462847106 28 VKDGYQWRKYGQKVTKDNPCPRAYFRCSFApACPVKkKQVEAAAPAGPSSEV 79 58*************************99*972579988766655555 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	16.398	23	64	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	2.7E-19	26	71	IPR003657	WRKY domain
SuperFamily	SSF118290	5.62E-17	27	68	IPR003657	WRKY domain
SMART	SM00774	1.2E-16	28	89	IPR003657	WRKY domain
Pfam	PF03106	1.1E-16	29	70	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 118 aa Download sequence Send to blast
GRRIREECKP KVSRRYVHAD PSDLSLVVKD GYQWRKYGQK VTKDNPCPRA YFRCSFAPAC 60 PVKKKQVEAA APAGPSSEVA RKNLAEHMAV TLTRDPGFKA ALVSALSGRI LELSPARD

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription repressor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:18175928, PubMed:15047897, PubMed:16623886). Represses specifically gibberellic acid (GA)-induced promoters in aleurone cells, probably by interfering with GAM1 (PubMed:15047897, PubMed:16623886, PubMed:19199048). Regulates, probably indirectly, the activation of defense-related genes such as GF14E during defense response (PubMed:18175928, PubMed:26851365, PubMed:26467468). Modulates plant innate immunity against X.oryzae pv. oryzae (Xoo) (PubMed:21961049). Confers resistance to the virulent bacterial pathogen Xoo 13751, probably via the regulation of NPR1 and PR1b defense signaling pathways (PubMed:16919842). {ECO:0000269\|PubMed:15047897, ECO:0000269\|PubMed:16623886, ECO:0000269\|PubMed:16919842, ECO:0000269\|PubMed:18175928, ECO:0000269\|PubMed:19199048, ECO:0000269\|PubMed:21961049, ECO:0000269\|PubMed:26467468, ECO:0000269\|PubMed:26851365}.
UniProt	Transcription repressor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Represses specifically gibberellic acid (GA)-induced promoters in aleurone cells, probably by interfering with GAM1 (PubMed:15047897). Regulates, probably indirectly, the activation of defense-related genes such as GF14E during defense response (By similarity). Modulates plant innate immunity against X.oryzae pv. oryzae (Xoo) (By similarity). Confers resistance to the virulent bacterial pathogen X.oryzae pv. oryzae (Xoo) 13751, probably via the regulation of NPR1 and PR1b defense signaling pathways (PubMed:16919842). {ECO:0000250\|UniProtKB:Q6QHD1, ECO:0000269\|PubMed:15047897, ECO:0000269\|PubMed:16919842}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	462847106

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by biotic elicitors (e.g. fungal chitin oligosaccharide and fungal cerebroside elicitors) and pathogen infection (e.g. the compatible pathogenic fungus M.grisea race 007, M.grisea crabgrass BR29). Accumulates in response to M.oryzae (By similarity). Triggered by defense signaling molecules, such as salicylic acid (SA), methyl jasmonate (MeJA), 1-aminocyclo-propane-1-carboxylic acid (ACC), wounding and pathogen infection (e.g. X.oryzae) (PubMed:16919842). Repressed by gibberellic acid (GA) (at protein level) (PubMed:15047897). Induced by abscisic acid (ABA) in aleurone cells, roots and leaves (PubMed:25110688). Accumulates in response to uniconazole, a GA biosynthesis inhibitor. Triggered strongly by cold in leaves, stems and developing spikes, but moderately by drought and salt stresses (By similarity). {ECO:0000250\|UniProtKB:Q6QHD1, ECO:0000269\|PubMed:15047897, ECO:0000269\|PubMed:16919842, ECO:0000269\|PubMed:25110688}.
UniProt	INDUCTION: Induced by biotic elicitors (e.g. fungal chitin oligosaccharide and fungal cerebroside elicitors) and pathogen infection (e.g. the compatible pathogenic fungus M.grisea race 007, M. grisea crabgrass BR29) (PubMed:18175928, PubMed:19772648, PubMed:23462973). Accumulates in response to M.oryzae (PubMed:21726398). Triggered by defense signaling molecules, such as salicylic acid (SA), methyl jasmonate (MeJA), 1-aminocyclo-propane-1-carboxylic acid (ACC), wounding and pathogen infection (e.g. X.oryzae) (PubMed:16919842). Repressed by gibberellic acid (GA) (at protein level) (PubMed:15047897, PubMed:19199048). Induced by abscisic acid (ABA) in aleurone cells, roots and leaves (PubMed:15618416, PubMed:16623886). Accumulates in response to uniconazole, a GA biosynthesis inhibitor (PubMed:16623886). Triggered strongly by cold in leaves, stems and developing spikes, but moderately by drought and salt stresses (PubMed:14645724, PubMed:23495849). {ECO:0000269\|PubMed:14645724, ECO:0000269\|PubMed:15047897, ECO:0000269\|PubMed:15618416, ECO:0000269\|PubMed:16623886, ECO:0000269\|PubMed:16919842, ECO:0000269\|PubMed:18175928, ECO:0000269\|PubMed:19199048, ECO:0000269\|PubMed:19772648, ECO:0000269\|PubMed:21726398, ECO:0000269\|PubMed:23462973, ECO:0000269\|PubMed:23495849}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KJ728369	2e-63	KJ728369.1 Zea mays clone pUT6651 WRKY transcription factor (WRKY124) mRNA, partial cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_015688476.1	1e-59	PREDICTED: LOW QUALITY PROTEIN: probable WRKY transcription factor 60
Swissprot	Q6IEL0	4e-40	WRK71_ORYSI; WRKY transcription factor WRKY71
Swissprot	Q6QHD1	4e-40	WRK71_ORYSJ; WRKY transcription factor WRKY71
TrEMBL	A0A3L6DD02	2e-60	A0A3L6DD02_MAIZE; WRKY transcription factor WRKY28
STRING	OB02G15470.1	2e-60	(Oryza brachyantha)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP1822	38	103

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G25000.1	1e-33	WRKY DNA-binding protein 60

Publications ? help Back to Top
Rabbani MA, et al. Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiol., 2003. 133(4): p. 1755-67 [PMID:14645724] Zhang Y,Wang L The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol. Biol., 2005. 5: p. 1 [PMID:15629062] Wu KL,Guo ZJ,Wang HH,Li J The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res., 2005. 12(1): p. 9-26 [PMID:16106749] Xie Z, et al. Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells. Plant J., 2006. 46(2): p. 231-42 [PMID:16623886] Zou X,Neuman D,Shen QJ Interactions of two transcriptional repressors and two transcriptional activators in modulating gibberellin signaling in aleurone cells. Plant Physiol., 2008. 148(1): p. 176-86 [PMID:18621977] Zhang ZL, et al. A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells. Plant Mol. Biol., 2009. 70(1-2): p. 139-51 [PMID:19199048] Berri S, et al. Characterization of WRKY co-regulatory networks in rice and Arabidopsis. BMC Plant Biol., 2009. 9: p. 120 [PMID:19772648] Delteil A, et al. Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence. Mol. Plant Pathol., 2012. 13(1): p. 72-82 [PMID:21726398] Peng Y,Bartley LE,Canlas P,Ronald PC OsWRKY IIa Transcription Factors Modulate Rice Innate Immunity. Rice (N Y), 2010. 3(1): p. 36-42 [PMID:21961049] Chujo T, et al. OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus. Plant Mol. Biol., 2013. 82(1-2): p. 23-37 [PMID:23462973] Kovalchuk N, et al. Optimization of TaDREB3 gene expression in transgenic barley using cold-inducible promoters. Plant Biotechnol. J., 2013. 11(6): p. 659-70 [PMID:23495849] Basu S,Roychoudhury A Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance. Biomed Res Int, 2014. 2014: p. 706890 [PMID:25110688] Liu Q, et al. OsGF14b Positively Regulates Panicle Blast Resistance but Negatively Regulates Leaf Blast Resistance in Rice. Mol. Plant Microbe Interact., 2016. 29(1): p. 46-56 [PMID:26467468] Liu Q, et al. OsGF14e positively regulates panicle blast resistance in rice. Biochem. Biophys. Res. Commun., 2016. 471(1): p. 247-52 [PMID:26851365]