PlantRegMap/PlantTFDB v5.0
Plant Transcription
Factor Database
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Home TFext BLAST Prediction Download Help About Links PlantRegMap |
Species | TF ID | Description |
---|---|---|
Pyrus communis (53) | PCP000360.1 | FAR1 family protein |
PCP002016.1 | FAR1 family protein | |
PCP002020.1 | FAR1 family protein | |
PCP002450.1 | FAR1 family protein | |
PCP004132.1 | FAR1 family protein | |
PCP004135.1 | FAR1 family protein | |
PCP004136.1 | FAR1 family protein | |
PCP004416.1 | FAR1 family protein | |
PCP004478.1 | FAR1 family protein | |
PCP005557.1 | FAR1 family protein | |
PCP005593.1 | FAR1 family protein | |
PCP005619.1 | FAR1 family protein | |
PCP006911.1 | FAR1 family protein | |
PCP008752.1 | FAR1 family protein | |
PCP008940.1 | FAR1 family protein | |
PCP009030.1 | FAR1 family protein | |
PCP009159.1 | FAR1 family protein | |
PCP014534.1 | FAR1 family protein | |
PCP017064.1 | FAR1 family protein | |
PCP018175.1 | FAR1 family protein | |
PCP018460.1 | FAR1 family protein | |
PCP019104.1 | FAR1 family protein | |
PCP019940.1 | FAR1 family protein | |
PCP019959.1 | FAR1 family protein | |
PCP023376.1 | FAR1 family protein | |
PCP024083.1 | FAR1 family protein | |
PCP024629.1 | FAR1 family protein | |
PCP024931.1 | FAR1 family protein | |
PCP025242.1 | FAR1 family protein | |
PCP026219.1 | FAR1 family protein | |
PCP026620.1 | FAR1 family protein | |
PCP027034.1 | FAR1 family protein | |
PCP027676.1 | FAR1 family protein | |
PCP027678.1 | FAR1 family protein | |
PCP028095.1 | FAR1 family protein | |
PCP030676.1 | FAR1 family protein | |
PCP031093.1 | FAR1 family protein | |
PCP031881.1 | FAR1 family protein | |
PCP033142.1 | FAR1 family protein | |
PCP033318.1 | FAR1 family protein | |
PCP035619.1 | FAR1 family protein | |
PCP035620.1 | FAR1 family protein | |
PCP036371.1 | FAR1 family protein | |
PCP038326.1 | FAR1 family protein | |
PCP038387.1 | FAR1 family protein | |
PCP038743.1 | FAR1 family protein | |
PCP038774.1 | FAR1 family protein | |
PCP039849.1 | FAR1 family protein | |
PCP040269.1 | FAR1 family protein | |
PCP041146.1 | FAR1 family protein | |
PCP043474.1 | FAR1 family protein | |
PCP043871.1 | FAR1 family protein | |
PCP044300.1 | FAR1 family protein |
We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885