PlantRegMap/PlantTFDB v5.0
Plant Transcription
Factor Database
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Home TFext BLAST Prediction Download Help About Links PlantRegMap |
Species | TF ID | Description |
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Citrus unshiu (60) | GAY32510.1 | FAR1 family protein |
GAY32514.1 | FAR1 family protein | |
GAY34223.1 | FAR1 family protein | |
GAY35402.1 | FAR1 family protein | |
GAY35765.1 | FAR1 family protein | |
GAY35766.1 | FAR1 family protein | |
GAY35768.1 | FAR1 family protein | |
GAY36143.1 | FAR1 family protein | |
GAY39572.1 | FAR1 family protein | |
GAY39828.1 | FAR1 family protein | |
GAY41404.1 | FAR1 family protein | |
GAY43658.1 | FAR1 family protein | |
GAY45870.1 | FAR1 family protein | |
GAY46181.1 | FAR1 family protein | |
GAY48386.1 | FAR1 family protein | |
GAY48497.1 | FAR1 family protein | |
GAY49067.1 | FAR1 family protein | |
GAY49346.1 | FAR1 family protein | |
GAY50055.1 | FAR1 family protein | |
GAY50056.1 | FAR1 family protein | |
GAY50057.1 | FAR1 family protein | |
GAY50863.1 | FAR1 family protein | |
GAY51487.1 | FAR1 family protein | |
GAY51644.1 | FAR1 family protein | |
GAY51645.1 | FAR1 family protein | |
GAY51648.1 | FAR1 family protein | |
GAY51649.1 | FAR1 family protein | |
GAY51650.1 | FAR1 family protein | |
GAY53605.1 | FAR1 family protein | |
GAY55380.1 | FAR1 family protein | |
GAY57081.1 | FAR1 family protein | |
GAY57121.1 | FAR1 family protein | |
GAY58483.1 | FAR1 family protein | |
GAY61175.1 | FAR1 family protein | |
GAY61582.1 | FAR1 family protein | |
GAY61635.1 | FAR1 family protein | |
GAY62766.1 | FAR1 family protein | |
GAY63865.1 | FAR1 family protein | |
GAY64419.1 | FAR1 family protein | |
GAY64459.1 | FAR1 family protein | |
GAY64460.1 | FAR1 family protein | |
GAY64818.1 | FAR1 family protein | |
GAY65014.1 | FAR1 family protein | |
GAY65325.1 | FAR1 family protein | |
GAY65495.1 | FAR1 family protein | |
GAY65497.1 | FAR1 family protein | |
GAY65498.1 | FAR1 family protein | |
GAY65499.1 | FAR1 family protein | |
GAY65633.1 | FAR1 family protein | |
GAY65647.1 | FAR1 family protein | |
GAY66370.1 | FAR1 family protein | |
GAY66371.1 | FAR1 family protein | |
GAY66372.1 | FAR1 family protein | |
GAY67703.1 | FAR1 family protein | |
GAY67704.1 | FAR1 family protein | |
GAY67800.1 | FAR1 family protein | |
GAY67801.1 | FAR1 family protein | |
GAY68387.1 | FAR1 family protein | |
GAY68621.1 | FAR1 family protein | |
GAY69297.1 | FAR1 family protein |
We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885