PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Triticum aestivum
ZF-HD Family
Species TF ID Description
TRAES3BF026100020CFD_t1ZF-HD family protein
TRAES3BF026200160CFD_t1ZF-HD family protein
TRAES3BF070300020CFD_t1ZF-HD family protein
TRAES3BF156400030CFD_t1ZF-HD family protein
Traes_1AL_0B583488F.1ZF-HD family protein
Traes_2BL_A36519520.2ZF-HD family protein
Traes_3AL_0D1B2D925.1ZF-HD family protein
Traes_4AL_5659286D11.1ZF-HD family protein
Traes_4AL_BC54910D5.2ZF-HD family protein
Traes_4BS_7322F0DC2.4ZF-HD family protein
Traes_4BS_7579785B4.1ZF-HD family protein
Traes_4DS_F3750ABFD.1ZF-HD family protein
Traes_4DS_F74D72DF1.1ZF-HD family protein
Traes_5AL_C1EC942D6.1ZF-HD family protein
Traes_5BL_6054E8EC9.1ZF-HD family protein
Traes_5BL_8A463E577.2ZF-HD family protein
Traes_5BL_93F0F3F5D.1ZF-HD family protein
Traes_5BL_B53DA57D4.2ZF-HD family protein
Traes_5DL_1F0881CE1.1ZF-HD family protein
Traes_5DL_9F8A19A00.1ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511