PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Salvia miltiorrhiza
HSF Family
Species TF ID Description
SMil_00000198-RA_SalvHSF family protein
SMil_00000807-RA_SalvHSF family protein
SMil_00001243-RA_SalvHSF family protein
SMil_00001418-RA_SalvHSF family protein
SMil_00001849-RA_SalvHSF family protein
SMil_00003117-RA_SalvHSF family protein
SMil_00003119-RA_SalvHSF family protein
SMil_00006017-RA_SalvHSF family protein
SMil_00006204-RA_SalvHSF family protein
SMil_00006271-RA_SalvHSF family protein
SMil_00006528-RA_SalvHSF family protein
SMil_00008026-RA_SalvHSF family protein
SMil_00008356-RA_SalvHSF family protein
SMil_00011473-RA_SalvHSF family protein
SMil_00012569-RA_SalvHSF family protein
SMil_00013026-RA_SalvHSF family protein
SMil_00013136-RA_SalvHSF family protein
SMil_00013284-RA_SalvHSF family protein
SMil_00015458-RA_SalvHSF family protein
SMil_00015559-RA_SalvHSF family protein
SMil_00016071-RA_SalvHSF family protein
SMil_00016518-RA_SalvHSF family protein
SMil_00019240-RA_SalvHSF family protein
SMil_00019365-RA_SalvHSF family protein
SMil_00019567-RA_SalvHSF family protein
SMil_00019949-RA_SalvHSF family protein
SMil_00020766-RA_SalvHSF family protein
SMil_00021923-RA_SalvHSF family protein
SMil_00022520-RA_SalvHSF family protein
SMil_00024017-RA_SalvHSF family protein
SMil_00025581-RA_SalvHSF family protein
SMil_00027348-RA_SalvHSF family protein
SMil_00029062-RA_SalvHSF family protein
SMil_00030400-RA_SalvHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645