PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Petunia axillaris
ZF-HD Family
Species TF ID Description
Peaxi162Scf00011g00209.1ZF-HD family protein
Peaxi162Scf00011g00321.1ZF-HD family protein
Peaxi162Scf00011g00739.1ZF-HD family protein
Peaxi162Scf00011g00748.1ZF-HD family protein
Peaxi162Scf00011g00820.1ZF-HD family protein
Peaxi162Scf00011g00821.1ZF-HD family protein
Peaxi162Scf00044g00198.1ZF-HD family protein
Peaxi162Scf00129g00014.1ZF-HD family protein
Peaxi162Scf00129g00133.1ZF-HD family protein
Peaxi162Scf00171g00086.1ZF-HD family protein
Peaxi162Scf00270g00002.1ZF-HD family protein
Peaxi162Scf00274g00617.1ZF-HD family protein
Peaxi162Scf00280g01015.1ZF-HD family protein
Peaxi162Scf00281g00094.1ZF-HD family protein
Peaxi162Scf00358g00081.1ZF-HD family protein
Peaxi162Scf00366g00031.1ZF-HD family protein
Peaxi162Scf00447g00082.1ZF-HD family protein
Peaxi162Scf00569g00114.1ZF-HD family protein
Peaxi162Scf00577g00036.1ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511