PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Medicago truncatula
M-type_MADS Family
Species TF ID Description
Medtr0061s0010.1M-type_MADS family protein
Medtr0121s0080.1M-type_MADS family protein
Medtr0121s0100.1M-type_MADS family protein
Medtr1g012570.1M-type_MADS family protein
Medtr1g041615.1M-type_MADS family protein
Medtr1g047550.1M-type_MADS family protein
Medtr1g054265.1M-type_MADS family protein
Medtr1g063160.1M-type_MADS family protein
Medtr1g075570.1M-type_MADS family protein
Medtr1g075600.1M-type_MADS family protein
Medtr1g077300.1M-type_MADS family protein
Medtr1g077320.1M-type_MADS family protein
Medtr1g077360.1M-type_MADS family protein
Medtr1g077390.1M-type_MADS family protein
Medtr1g084950.1M-type_MADS family protein
Medtr1g090697.1M-type_MADS family protein
Medtr1g090710.1M-type_MADS family protein
Medtr1g090783.1M-type_MADS family protein
Medtr1g105905.1M-type_MADS family protein
Medtr1g105910.1M-type_MADS family protein
Medtr1g105920.1M-type_MADS family protein
Medtr1g106070.1M-type_MADS family protein
Medtr1g108500.1M-type_MADS family protein
Medtr1g108510.1M-type_MADS family protein
Medtr1g108580.1M-type_MADS family protein
Medtr1g114730.1M-type_MADS family protein
Medtr2g016210.1M-type_MADS family protein
Medtr2g030740.1M-type_MADS family protein
Medtr2g035580.1M-type_MADS family protein
Medtr2g035610.1M-type_MADS family protein
Medtr2g045020.1M-type_MADS family protein
Medtr2g049610.1M-type_MADS family protein
Medtr2g085250.1M-type_MADS family protein
Medtr2g085280.1M-type_MADS family protein
Medtr2g093190.1M-type_MADS family protein
Medtr2g105290.1M-type_MADS family protein
Medtr2g461710.1M-type_MADS family protein
Medtr3g014350.1M-type_MADS family protein
Medtr3g030770.1M-type_MADS family protein
Medtr3g030780.1M-type_MADS family protein
Medtr3g031100.1M-type_MADS family protein
Medtr3g031240.1M-type_MADS family protein
Medtr3g052870.1M-type_MADS family protein
Medtr3g052920.1M-type_MADS family protein
Medtr3g065100.1M-type_MADS family protein
Medtr3g067870.1M-type_MADS family protein
Medtr3g067875.1M-type_MADS family protein
Medtr3g067910.1M-type_MADS family protein
Medtr3g080940.1M-type_MADS family protein
Medtr3g093900.1M-type_MADS family protein
Medtr3g102570.1M-type_MADS family protein
Medtr3g109930.1M-type_MADS family protein
Medtr3g437790.1M-type_MADS family protein
Medtr3g466830.1M-type_MADS family protein
Medtr3g466890.1M-type_MADS family protein
Medtr3g466980.1M-type_MADS family protein
Medtr3g467080.1M-type_MADS family protein
Medtr4g019670.1M-type_MADS family protein
Medtr4g028720.1M-type_MADS family protein
Medtr4g028800.1M-type_MADS family protein
Medtr4g031910.1M-type_MADS family protein
Medtr4g032260.1M-type_MADS family protein
Medtr4g032290.1M-type_MADS family protein
Medtr4g032620.1M-type_MADS family protein
Medtr4g036915.1M-type_MADS family protein
Medtr4g051538.1M-type_MADS family protein
Medtr4g063790.1M-type_MADS family protein
Medtr4g093030.1M-type_MADS family protein
Medtr4g094632.1M-type_MADS family protein
Medtr4g094638.1M-type_MADS family protein
Medtr4g107170.1M-type_MADS family protein
Medtr4g108720.1M-type_MADS family protein
Medtr4g108720.2M-type_MADS family protein
Medtr4g108720.3M-type_MADS family protein
Medtr4g127140.1M-type_MADS family protein
Medtr4g131030.1M-type_MADS family protein
Medtr5g045560.1M-type_MADS family protein
Medtr5g046870.1M-type_MADS family protein
Medtr5g047580.1M-type_MADS family protein
Medtr5g053390.1M-type_MADS family protein
Medtr5g055100.1M-type_MADS family protein
Medtr5g066960.1M-type_MADS family protein
Medtr5g075380.1M-type_MADS family protein
Medtr6g005440.1M-type_MADS family protein
Medtr6g005450.1M-type_MADS family protein
Medtr6g018920.1M-type_MADS family protein
Medtr7g011950.1M-type_MADS family protein
Medtr7g028448.1M-type_MADS family protein
Medtr7g055790.1M-type_MADS family protein
Medtr7g055940.1M-type_MADS family protein
Medtr7g062350.1M-type_MADS family protein
Medtr7g106510.1M-type_MADS family protein
Medtr8g022970.1M-type_MADS family protein
Medtr8g036130.1M-type_MADS family protein
Medtr8g043650.1M-type_MADS family protein
Medtr8g046350.1M-type_MADS family protein
Medtr8g051580.1M-type_MADS family protein
Medtr8g079502.1M-type_MADS family protein
Medtr8g086290.1M-type_MADS family protein
M-type_MADS (M-type MADS) Family Introduction

The best studied plant MADS-box transcription factors are those involved in floral organ identity determination. Analysis of homeotic floral mutants resulted in the formulation of a genetic model, named the ABC model, that explains how the combined functions of three classes of genes (A, B, and C) determine the identity of the four flower organs (reviewed by Coen and Meyerowitz, 1991). Arabidopsis has two A-class genes (AP1 and AP2 [Bowman et al., 1989]), two B-class genes (PI and AP3), and a single C-class gene (AG), of which only AP2 is not a MADS-box gene. Recently, it was shown that the Arabidopsis B- and C-function genes, which control petal, stamen, and carpel development, are functionally dependent on three highly similar MADS-box genes, SEP1, SEP2, and SEP3 (Pelaz et al., 2000). Interestingly, only when mutant knockout alleles of the three SEP genes were combined in a triple sep1 sep2 sep3 mutant was loss of petal, stamen, and carpel identity observed, resulting in a flower composed of only sepals. This example shows that redundancy occurs in the MADS-box gene family, which complicates reverse genetic strategies for gene function analysis. The SHP genes provide another example of MADS-box gene redundancy. shp1 and shp2 single mutants do not exhibit any phenotypic effect, whereas in the double mutant, development of the dehiscence zone is disturbed in the fruit, resulting in a failure to release seeds (Liljegren et al., 2000)[1].

It has been proposed that there are at least 2 lineages (type I and type II) of MADS-box genes in plants, animals, and fungi. Most of the well-studied plant genes are type II genes and have three more domains than type I genes from the N to the C terminus of the protein:intervening (I) domain (~30 codons), keratin-lik e coiled-coil (K) domain (~70 codons), and Cterminal (C) domain (variable length). These genes are called the MIKC-type and are specific to plants[2].

The MADS-box is a DNA binding domain of 58 amino acids that binds DNA at consensus recognition sequences known as CArG boxes [CC(A/T)6GG] (Hayes et al., 1988; Riechmann et al., 1996b). The interaction with DNA has been studied in detail for the human and yeast MADS-box proteins thanks to the resolved crystal structures (Pellegrini et al., 1995; Santelli and Richmond, 2000). The I domain is less conserved and contributes to the specification of dimerization. The K domain is characterized by a coiled-coil structure, which facilitates the dimerization of MADS-box proteins (Davies et al., 1996; Fan et al., 1997). The C domain is the least conserved domain; in some cases, it has been shown to contain a transactivation domain or to contribute to the formation of multimeric MADS-box protein complexes (Egea-Cortines et al., 1999; Honma and Goto, 2001)[1].

1.Parenicova L, de Folter S, Kieffer M, Horner DS, Favalli C, Busscher J, Cook HE, Ingram RM, Kater MM, Davies B, Angenent GC, Colombo L.
Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world.
Plant Cell. 2003 Jul;15(7):1538-51.
PMID: 12837945
2.Nam J, dePamphilis CW, Ma H, Nei M.
Antiquity and evolution of the MADS-box gene family controlling flower development in plants.
Mol Biol Evol. 2003 Sep;20(9):1435-47. Epub 2003 May 30.
PMID: 12777513