PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Lotus japonicus
ZF-HD Family
Species TF ID Description
Lj0g3v0058889.1ZF-HD family protein
Lj0g3v0224609.1ZF-HD family protein
Lj0g3v0229569.1ZF-HD family protein
Lj0g3v0229739.1ZF-HD family protein
Lj0g3v0268869.1ZF-HD family protein
Lj0g3v0268879.1ZF-HD family protein
Lj0g3v0306449.1ZF-HD family protein
Lj0g3v0321019.1ZF-HD family protein
Lj1g3v0244390.1ZF-HD family protein
Lj1g3v0491080.1ZF-HD family protein
Lj1g3v1638760.1ZF-HD family protein
Lj1g3v2925250.1ZF-HD family protein
Lj2g3v0635660.1ZF-HD family protein
Lj2g3v1058090.1ZF-HD family protein
Lj2g3v1058120.1ZF-HD family protein
Lj2g3v1277940.1ZF-HD family protein
Lj2g3v1439670.1ZF-HD family protein
Lj2g3v1745000.1ZF-HD family protein
Lj2g3v1757070.1ZF-HD family protein
Lj2g3v1757080.1ZF-HD family protein
Lj4g3v0015460.1ZF-HD family protein
Lj4g3v0962260.1ZF-HD family protein
Lj4g3v0962270.1ZF-HD family protein
Lj4g3v2313490.1ZF-HD family protein
Lj5g3v0526310.1ZF-HD family protein
Lj5g3v2258260.1ZF-HD family protein
Lj5g3v2258280.1ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511