PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Ipomoea trifida
ZF-HD Family
Species TF ID Description
Itr_sc000002.1_g00059.1ZF-HD family protein
Itr_sc000021.1_g00029.1ZF-HD family protein
Itr_sc000125.1_g00064.1ZF-HD family protein
Itr_sc000144.1_g00010.1ZF-HD family protein
Itr_sc000148.1_g00022.1ZF-HD family protein
Itr_sc000225.1_g00014.1ZF-HD family protein
Itr_sc000277.1_g00014.1ZF-HD family protein
Itr_sc000341.1_g00027.1ZF-HD family protein
Itr_sc000389.1_g00005.1ZF-HD family protein
Itr_sc000707.1_g00018.1ZF-HD family protein
Itr_sc000727.1_g00002.1ZF-HD family protein
Itr_sc000727.1_g00004.1ZF-HD family protein
Itr_sc000769.1_g00011.1ZF-HD family protein
Itr_sc000779.1_g00003.1ZF-HD family protein
Itr_sc000813.1_g00027.1ZF-HD family protein
Itr_sc001068.1_g00007.1ZF-HD family protein
Itr_sc002012.1_g00002.1ZF-HD family protein
Itr_sc002093.1_g00004.1ZF-HD family protein
Itr_sc002272.1_g00005.1ZF-HD family protein
Itr_sc002970.1_g00007.1ZF-HD family protein
Itr_sc004392.1_g00001.1ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511