PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Eragrostis tef
HSF Family
Species TF ID Description
462848079HSF family protein
462848776HSF family protein
462848892HSF family protein
462857966HSF family protein
462859754HSF family protein
462865282HSF family protein
462868440HSF family protein
462868449HSF family protein
462878678HSF family protein
462878696HSF family protein
462879653HSF family protein
462882586HSF family protein
462883510HSF family protein
462885445HSF family protein
462887679HSF family protein
462888082HSF family protein
462894315HSF family protein
462897384HSF family protein
462905084HSF family protein
462910900HSF family protein
462912523HSF family protein
462912854HSF family protein
462916315HSF family protein
462917328HSF family protein
462920806HSF family protein
462921327HSF family protein
462932556HSF family protein
462932869HSF family protein
462934678HSF family protein
462936521HSF family protein
462938686HSF family protein
462938689HSF family protein
462960124HSF family protein
462969128HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645