PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Eragrostis tef
FAR1 Family
Species TF ID Description
462849598FAR1 family protein
462854976FAR1 family protein
462854985FAR1 family protein
462865514FAR1 family protein
462867543FAR1 family protein
462879359FAR1 family protein
462880771FAR1 family protein
462880773FAR1 family protein
462885349FAR1 family protein
462896261FAR1 family protein
462897343FAR1 family protein
462902751FAR1 family protein
462902783FAR1 family protein
462914309FAR1 family protein
462914403FAR1 family protein
462918999FAR1 family protein
462919968FAR1 family protein
462922416FAR1 family protein
462929299FAR1 family protein
462929323FAR1 family protein
462931646FAR1 family protein
462931984FAR1 family protein
462932193FAR1 family protein
462935663FAR1 family protein
462940887FAR1 family protein
462944111FAR1 family protein
462946865FAR1 family protein
462949760FAR1 family protein
462951063FAR1 family protein
462955422FAR1 family protein
462966920FAR1 family protein
462966958FAR1 family protein
462968259FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885