PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Coffea canephora
HSF Family
Species TF ID Description
Cc00_g20610HSF family protein
Cc00_g28360HSF family protein
Cc00_g30710HSF family protein
Cc01_g19020HSF family protein
Cc02_g12390HSF family protein
Cc02_g13270HSF family protein
Cc02_g15780HSF family protein
Cc02_g17130HSF family protein
Cc02_g32920HSF family protein
Cc03_g04280HSF family protein
Cc03_g08510HSF family protein
Cc05_g09000HSF family protein
Cc06_g05260HSF family protein
Cc06_g09660HSF family protein
Cc06_g11570HSF family protein
Cc06_g17660HSF family protein
Cc06_g19040HSF family protein
Cc07_g05960HSF family protein
Cc08_g03200HSF family protein
Cc08_g05360HSF family protein
Cc08_g05370HSF family protein
Cc08_g10810HSF family protein
Cc08_g14180HSF family protein
Cc10_g04530HSF family protein
Cc11_g06670HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645