PlantRegMap/PlantTFDB v5.0
Plant Transcription
Factor Database
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Home TFext BLAST Prediction Download Help About Links PlantRegMap |
Species | TF ID | Description |
---|---|---|
Achn000081 | FAR1 family protein | |
Achn000821 | FAR1 family protein | |
Achn002281 | FAR1 family protein | |
Achn002531 | FAR1 family protein | |
Achn012651 | FAR1 family protein | |
Achn014121 | FAR1 family protein | |
Achn018201 | FAR1 family protein | |
Achn019771 | FAR1 family protein | |
Achn020651 | FAR1 family protein | |
Achn030321 | FAR1 family protein | |
Achn032341 | FAR1 family protein | |
Achn044611 | FAR1 family protein | |
Achn046071 | FAR1 family protein | |
Achn058751 | FAR1 family protein | |
Achn065071 | FAR1 family protein | |
Achn065501 | FAR1 family protein | |
Achn067421 | FAR1 family protein | |
Achn069111 | FAR1 family protein | |
Achn071481 | FAR1 family protein | |
Achn074601 | FAR1 family protein | |
Achn078441 | FAR1 family protein | |
Achn078551 | FAR1 family protein | |
Achn088571 | FAR1 family protein | |
Achn093351 | FAR1 family protein | |
Achn094131 | FAR1 family protein | |
Achn097861 | FAR1 family protein | |
Achn100281 | FAR1 family protein | |
Achn128261 | FAR1 family protein | |
Achn139261 | FAR1 family protein | |
Achn154831 | FAR1 family protein | |
Achn156891 | FAR1 family protein | |
Achn158101 | FAR1 family protein | |
Achn163291 | FAR1 family protein | |
Achn164231 | FAR1 family protein | |
Achn165991 | FAR1 family protein | |
Achn173641 | FAR1 family protein | |
Achn176661 | FAR1 family protein | |
Achn176741 | FAR1 family protein | |
Achn204981 | FAR1 family protein | |
Achn205991 | FAR1 family protein | |
Achn209551 | FAR1 family protein | |
Achn210321 | FAR1 family protein | |
Achn211241 | FAR1 family protein | |
Achn211251 | FAR1 family protein | |
Achn213491 | FAR1 family protein | |
Achn224681 | FAR1 family protein | |
Achn231181 | FAR1 family protein | |
Achn232491 | FAR1 family protein | |
Achn236831 | FAR1 family protein | |
Achn237121 | FAR1 family protein | |
Achn242741 | FAR1 family protein | |
Achn247721 | FAR1 family protein | |
Achn250031 | FAR1 family protein | |
Achn253161 | FAR1 family protein | |
Achn258341 | FAR1 family protein | |
Achn263121 | FAR1 family protein | |
Achn264271 | FAR1 family protein | |
Achn264411 | FAR1 family protein | |
Achn267741 | FAR1 family protein | |
Achn272241 | FAR1 family protein | |
Achn275271 | FAR1 family protein | |
Achn275581 | FAR1 family protein | |
Achn284071 | FAR1 family protein | |
Achn287391 | FAR1 family protein | |
Achn290481 | FAR1 family protein | |
Achn292641 | FAR1 family protein | |
Achn310101 | FAR1 family protein | |
Achn314731 | FAR1 family protein | |
Achn314811 | FAR1 family protein | |
Achn317851 | FAR1 family protein | |
Achn317861 | FAR1 family protein | |
Achn324281 | FAR1 family protein | |
Achn325861 | FAR1 family protein | |
Achn327871 | FAR1 family protein | |
Achn327891 | FAR1 family protein | |
Achn327901 | FAR1 family protein | |
Achn337331 | FAR1 family protein | |
Achn343591 | FAR1 family protein | |
Achn345551 | FAR1 family protein | |
Achn346681 | FAR1 family protein | |
Achn360501 | FAR1 family protein | |
Achn370431 | FAR1 family protein | |
Achn372231 | FAR1 family protein | |
Achn376681 | FAR1 family protein | |
Achn376871 | FAR1 family protein | |
Achn377091 | FAR1 family protein | |
Achn377111 | FAR1 family protein | |
Achn391061 | FAR1 family protein |
We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885